Introduction and Objective:
Ultraviolet light in the UV-C band is known as germicidal radiation and was widely used for both sterilization of the equipment and creation of a sterile environment. The aim of the study is to assess the effectiveness of inactivation of microorganisms deposited on surfaces with various textures by UV-C radiation disinfection devices.

Material and Methods:
Five microorganisms (3 bacteria, virus, and fungus) deposited on metal, plastic, and glass surfaces with smooth and rough textures were irradiated with UV-C light emitted by low-pressure mercury lamp and ultraviolet emitting diodes (LEDs), from a distance of 0.5 m, 1 m, and 1.5 m to check their survivability after 20-minute exposure.

Results and Conclusions:
Both tested UV-C sources were effective in inactivation of microorganisms; however, LED emitter was more efficient in this respect than the mercury lamp. The survival rate of microorganisms depended on the UV-C dose, conditioned by the distance from UV-C source being the highest at 0.5 m and the lowest at 1.5 m. For the tested microorganisms, the highest survival rate after UV-C irradiation was usually visible on glass and plastic surfaces. This observation should be considered in all environments where the type of material (from which the elements of technical equipment are manufactured and may be contaminated by specific activities) is important for maintaining the proper level of hygiene and avoiding the unwanted and uncontrolled spread of microbiological pollution.

This paper was created on the basis of results of a research task carried out within the scope of the 6th stage of the “Governmental Programme for Improvement of Safety and Working Conditions” funded by the resources of the National Centre for Research and Development (task no. I.PN.13 entitled “Test methods and criteria for assessing devices used for disinfection with UV-C radiation in the work environment and non-industrial indoor environment in terms of their safety of use and effectiveness of microbial inactivation”). The Central Institute for Labour Protection – National Research Institute is the Programme’s main coordinator.
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