RESEARCH PAPER
Effect of a plant preparation Citrosept on selected immunity indices in blood of slaughter turkey hens
| 1 | Department of Biochemistry and Toxicology, Faculty of Animal Biology and Breeding University of Life Sciences, Lublin, Poland |
| 2 | Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland |
CORRESPONDING AUTHOR
Elzbieta Rusinek-Prystupa
Department of Biochemistry and Toxicology, Faculty of Animal Biology and Breeding University of Life Sciences, Lublin, Poland
Department of Biochemistry and Toxicology, Faculty of Animal Biology and Breeding University of Life Sciences, Lublin, Poland
Ann Agric Environ Med. 2014;21(3):581–584
KEYWORDS
ABSTRACT
Introduction and objective:
The objective of this study was to determine the effect of per os administration of 3 various dosages of a Citrosept preparation (a grapefruit extract)to growing turkey hens on changes in their selected haematological and immunological blood indices. An attempt was also undertaken to select the most efficient dose of the preparation with respect to the mentioned indices in turkey hens.
Material and Methods:
The experiment was conducted on 180 turkey hens allocated at random to 4 groups, 45 birds in each group. Samples of their full blood were analyzed for haematological indices, such as red blood cell count (RBS), haemoglobin content (Hb), haematocrit value (Ht), and white blood cell count (WBC). Samples of blood plasma were assayed to determine the activity of lysozyme (chamber-diffusive method) and heterophils capability to reduce nitro blue tetrazolium (stimulated and spontaneous NBT test). Phagocytic activity of leucocytes against Staphylococcus aureus 209P strain was assessed and expressed as the percentage of phagocytic cells (% PC) and phagocytic index (PI).
Results:
The administration of the grapefruit extract to turkey hens with drinking water caused a significant increase in haemoglobin content in blood, as well as an increase in non-specific humoral immunity marker (activity of lysozyme) and non-specific cellular immunity marker (percentage of phagocytic cells; P ≤ 0.05).
Conclusions:
The results obtained enabled the positive evaluation of the advisability of applying the Citrosept preparation in the feeding of turkey hens at the age of 6–9 weeks. Among the doses examined, the most efficient with respect to the stimulation of the non-specific humoral and cellular immunity was the dose of 0.021 ml/kg of body weight.
The objective of this study was to determine the effect of per os administration of 3 various dosages of a Citrosept preparation (a grapefruit extract)to growing turkey hens on changes in their selected haematological and immunological blood indices. An attempt was also undertaken to select the most efficient dose of the preparation with respect to the mentioned indices in turkey hens.
Material and Methods:
The experiment was conducted on 180 turkey hens allocated at random to 4 groups, 45 birds in each group. Samples of their full blood were analyzed for haematological indices, such as red blood cell count (RBS), haemoglobin content (Hb), haematocrit value (Ht), and white blood cell count (WBC). Samples of blood plasma were assayed to determine the activity of lysozyme (chamber-diffusive method) and heterophils capability to reduce nitro blue tetrazolium (stimulated and spontaneous NBT test). Phagocytic activity of leucocytes against Staphylococcus aureus 209P strain was assessed and expressed as the percentage of phagocytic cells (% PC) and phagocytic index (PI).
Results:
The administration of the grapefruit extract to turkey hens with drinking water caused a significant increase in haemoglobin content in blood, as well as an increase in non-specific humoral immunity marker (activity of lysozyme) and non-specific cellular immunity marker (percentage of phagocytic cells; P ≤ 0.05).
Conclusions:
The results obtained enabled the positive evaluation of the advisability of applying the Citrosept preparation in the feeding of turkey hens at the age of 6–9 weeks. Among the doses examined, the most efficient with respect to the stimulation of the non-specific humoral and cellular immunity was the dose of 0.021 ml/kg of body weight.
REFERENCES (26)
1.
Sharon S, Bagiński BJ. Therapeutic strength of an extract from grapefruit. Warszawa, Oficyna Wydawnicza „MB”, 1998.
2.
Kędzia A. Citrosept activity on anaerobic bacteria isolated from respiratory tract infections. Post Fitoter. 2000; 3: 11–14.
3.
Galanty A, Janeczko Z. Examination of Citrosept influence on cancer cells vitality in vitro. Post Fitoter. 2003; 1: 16–18.
4.
Rusinek E. The influence of extract from grapefruit (Citrus Paradisi) and the root of Scutellaria baicalensis on hematological and biochemical indicators and production effects of turkey females. Doctoral thesis. Lublin, University of Life Sciences, 2012.
6.
Mielczarek C. Analysis of acidic and basic properties of rutin and quercetin. Farm Pol. 2003; 59(1): 11–19.
7.
Rypuła K, Jopek Z, Śmielewska-Łoś E, Klimentowski S, Kucharczak E. Grapefruit extract in stabilization of bacterial flora in poultry alimentary tract. Electronic. EJPAU. 2003; 6(2): 8.
8.
Smulikowska S, Rutkowski A. Poultry Feeding Standards. Feeding guidelines and nutritive value of feed mixtures. 4th ed. Jabłonna, IFiŻZ PAN, 2005.
9.
Park BH, Fikrig SM, Smithuick E. M. Infection and nitroblue tetrazolium reduction by neutrophils. Lancet 1968; 2(7567): 532–534.
10.
Hankiewicz J, Świerczek E. Studies on serum and urinary lysozyme. Pol Arch Med Wewn. 1974; 51(6): 591–597.
11.
Zaporowska H. Trace elements in animals nutrition and human. Lublin, Wydawnictwo UMCS, 2002.
12.
Ognik K, Sembratowicz I, Modzelewska-Banachiewicz B. Concentrations of selected elements and activities of anti-oxidation enzymes in blood of turkey hens given Echinovit C and 1,2,4-triazole derivative. J Element. 2004; 9(3): 445–449.
13.
Sembratowicz I. Influence of herbal extracts on some parameters of blood and performance of turkey hens. Ann UMCS EE. 2004; 22(43): 325–332.
14.
Sembratowicz I, Ognik K, Truchliński J, Cendrowska M. Content of minerals in blond serum and tissues of turkey hens supplemented with Biostymina and Biotron C. J Element. 2004; 9(4): 743–748.
15.
Truchliński J, Ognik K, Sembratowicz I, Smelkowska B. The influence of plant preparation Ginsengin 200 on some hematological and biochemical parameters and performance of turkey hens. Ann UMCS EE. 2005; 23(45): 344–348.
16.
Majewska T, Mikulski D, Święcicka-Grabowska, G, Wójcik R. Water extracts of fresh garlic in the nutrition of young slaughter turkeys. Medycyna Wet. 2007; 63(11): 1357–1360.
17.
Powell PC. Immune mechanisms in infections in poultry. Vet Immunol Immunopathol. 1987; 15(1–2): 87–113.
18.
Sembratowicz I, Ognik K, Truchliński J. Influence of Biostymina, Biotron C on some immunological parameters and performance of turkey hens. Ann UMCS EE. 2004; 22(42): 317–323.
19.
Truchliński J, Krauze M, Cendrowska-Pinkosz M, Modzelewska-Banachiewicz B. Influence of garlic, synthetic 1,2,4 – triazole derivative and herbal preparation Echinovit C on selected indices of turkey – hens non – specific immunity. Pol J Vet Sci. 2006; 9(1): 51–55.
20.
Krauze M, Truchliński J, Adamczyk M, Cendrowska-Pinkosz M. Effectiveness of rearing Big-6 turkey-hens for slaughter fed with garlic, coneflower preparation and 1,2,4-triasole derivative additions. Ann UMCS DD. 2007; 62(2): 7–14.
21.
Dudek K, Tatara MR, Śliwa E, Siwicki AK, Łuszczewska-Sierakowska I, Zipser J, Krupski W, Studziński T. Effects of perinatal administration of aged garlic extract (AGE) and allicin on non-specific and specific defence mechanisms in sows. Pol J Environ Stud. 2005; 14(Suppl. 2): 69–72.
22.
Tatara MR, Śliwa E, Dudek K, Siwicki AK, Kowalik S, Łuszczewska-Sierakowska I, Krupski W, Zipser J, Studziński T. Influence of perinatal administration of aged garlic extract (AGE) and allicin to sows on some defence mechanisms in their piglets during postnatal life. Pol J Environ Stud. 2005; 14(Suppl. 2): 378–381.
23.
Dudek K, Śliwa E, Tatara MR. Changes in blood leukocyte patter in piglets from sows treated with garlic preparations. Bull Vet Inst Pulawy 2006; 50(2): 263–267.
24.
Heggers JP, Cottingham J, Gusman J, Reagor L, Mccoy L, Carino E, Cox R, Zhao JG. The effectiveness of processed grapefruit-seed extract as an antibacterial agent: II. Mechanism of action and in vitro toxicity. J Altern Complement Med. 2002; 8(3): 333–340.
26.
Jarosz Ł, Wójcicka-Lorenowicz K, Majer-Dziedzic B, Kostro K, Mikołajczak M. Clinical observations on the efficacy of Isoprivet for unspecific immunoprophylactic in streptococcosis of pigs. Ann UMCS DD. 2008; 63(3): 16–23.
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.