Concentration of fungal biomass and trichothecenes in different parts of einkorn.
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Department of Chemistry, Poznań Univerity of Life Sciences, Poznań, Poland
Department of Plant Breeding and Seed Production, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo, Poland
Ann Agric Environ Med. 2011;18(1):89-98
Analyses of ergosterol (ERG), adenosine-5?-triphosphate (ATP) and groups A and B trichothecenes were performed in three Triticum monococcum cultivars registered in Germany (Albini, Terzino and Tifi), grown in the organic system. The experiment was carried out on two dates: the first - in the final phase of flowering (BBCH 69) and the second - in the phase of full ripeness (BBCH 89). The analyses were performed on shanks, glumes, grain and awns. Concentrations of analyzed metabolites in different parts of T. monococcum plants varied significantly. Mean ERG concentration in the first term was more than 30 times higher than immediately before harvest, whereas for group B trichothecenes it was 4 times higher. Contents of ATP and concentrations of group A trichothecenes were similar at both times. When analyzing parts of the spike, the highest amount of metabolites was recorded in shanks (ERG - 114 mg/kg, ATP 900,000 RLU, group A and B trichothecenes - 0.07 and 0.20 mg/kg, respectively), while the lowest in grain (ERG - 5 mg/kg, ATP 55,000 RLU, group A and B trichothecenes - 0.03 and 0.08 mg/kg, respectively). A higher ERG concentration was found in awns (65 mg/kg) than in glumes (41 mg/kg), whereas for ATP and group A and B trichothecenes by contrast higher concentrations were recorded in glumes (160,000 RLU, 0.06 and 0.029 mg/kg, respectively) than in awns (77,000 RLU, 0.05 and 0.014 mg/kg, respectively). Recorded results indicate a potential occurrence of trichothecenes in shanks, awns and glumes already during flowering, when grain has not yet developed. In these parts of plants, after harvest, the highest amounts of microorganisms and Fusarium toxins were found, which pose a threat for farmers and workers employed in the cereal industry.
Abelho M: ATP and ergosterol as indicators of fungal biomass during leaf decomposition in streams: a comparative study. Int Rev Hydrobiol 2009, 94, 3–15.
Asam S, Rychlik M: Studies on accuracy of trichothecene multitoxin analysis using stable isotope dilution assays. Mycotoxin Res 2007, 23(4), 191–198.
Audilakshmi S, Stenhouse JW, Reddy TP, Prasad MVR: Grain mould resistance and associated characters of sorghum genotypes. Euphytica 1999, 107, 91–103.
Beyer M, Klix MB, Verreet J-A: Estimating mycotoxin contents of Fusarium-damaged winter wheat kernels. Int J Food Microbiol 2007, 119, 153–158.
Bíro D, Juráček M, Kačániová M, Šimko M, Gálik B, Michálková J, Gyöngyová E: Occurrence of microscopic fungi and mycotoxins in conserved high moisture corn from Slovakia. Ann Agric Environ Med 2009, 16, 227–232.
Boutigny AL, Richard-Forget F, Barreau C: Natural mechanisms for cereal resistance to the accumulation of Fusarium trichothecenes. Eur J Plant Pathol 2008, 121, 411–423.
Buśko M, Perkowski J, Wiwart M, Góral T, Suchowilska E, Stuper K, Matysiak A: Kinetics of fungal metabolites formation after inoculation of wheat spikes with F. culmorum. Cereal Res Commun 2008, 36, 443–449.
Buttinger G, Krska R: Determination of B-trichothecenes in wheat by post column derivatisation liquid chromatography with fl uorescence detection (PCD-HPLC-FLD). Mycotoxin Res 2003, 19, 139–143.
Guan S, He J, Young JCH, Zhu H, Li H-Z, Ji CH, Zhou T: Transformation of trichothecene mycotoxins by microorganisms from fi sh digesta. Aquaculture 2009, 3, 290–295.
He J, Zhou T, Young JCH, Boland GJ, Scott PM: Chemical and biological transformations for detoxifi cation of trichothecene mycotoxins in human and animal food chains. Trends Food Sci Technol 2010, 21, 67–76.
Krysińska-Traczyk E, Kiecana I, Perkowski J, Dutkiewicz J: Levels of fungi and mycotoxins in samples of grain and grain dust collected on farms in eastern Poland. Ann Agric Environ Med 2001, 8, 269–274.
Lemmens M, Haim K, Lew H, Ruckenbauer P: The effect of nitrogen fertilizer on Fusarium head blight development and deoxynivalenol contamination in wheat. J Phytopathol 2004, 152, 1–8.
Lugauskas A, Raila A, Zvicevicius E, Railiene M, Novosinskas H: Factors determining accumulation of mycotoxin producers in cereal grain during harvesting. Ann Agric Environ Med 2007, 14(1), 173–86.
Maupetit P, Gatel F, Cahagnier B, Botorel G, Charlier M, Collet B, Dauvillier P, Laffi teau J, Roux G: Quantitative estimation of fungal infestation of feedstuffs by determining ergosterol content. 44th Annual Meeting of EAAP Aarhus, 16–19. Denmark 1993.
Mesterházy A: Role of deoxynivalenol in aggressiveness of Fusarium graminearum and F. culmorum and in resistance to Fusarium head blight. Eur J Plant Pathol 2002, 108, 675–684.
Perkowski J, Basiński T, Wiwart M, Kostecki M, Buśko M, Matysiak A: The effect of environmental conditions on ergosterol and trichothecene content of naturally contaminated oat grain. Ann Agric Environ Med 2008, 15, 271–276.
Perkowski J, Buśko M, Stuper K, Kostecki M, Matysiak A, Szwajkowska-Michałek L: Concentration of ergosterol in small-grained naturally contaminated and inoculated cereals. Biologia 2008, 63(4), 542–547.
Perkowski J, Kiecana I, Kaczmarek Z: Natural occurrence and distribution of Fusarium toxins in contaminated barley cultivars. Eur J Plant Pathol 2003, 109, 331–339.
Perkowski J, Wiwart M, Buśko M, Laskowska M, Berthiller F, Kandler W, Krska R: Fusarium toxins and total fungal biomass indicators in naturally contaminated wheat samples from north-eastern Poland in 2003. Food Addit Contam 2007, 24, 1292–1298.
Piecková E, Jesenská Z: Microscopic fungi in dwellings and their health implications in humans. Ann Agric Environ Med 1999, 6, 1–11.
Schnürer J, Jansson A: Ergosterol levels and mould colony forming units in Swedish grain of food and feed grade. Acta Agr Scand B 1992, 42, 240–245.
Stuper K, Perkowski J: Dynamika wzrostu pleśni oraz tworzenie mikotoksyn podczas przechowywania chleba. Aparat Bad Dydakt 2010, 3, 25–31.
Suberkropp K, Gessner MO, Chauvet E: Comparison of ATP and Ergosterol as Indicators of Fungal Biomass Associated with Decomposing Leaves in Streams. Appl Environ Microbiol 1993, 59(10), 3367–3372.
Suchowilska E, Kandler W, Sulyok M, Wiwart M, Krska R: Mycotoxin profi les in the grain of Triticum monococcum, Triticum dicoccum and Triticum spelta after head infection with Fusarium culmorum. J Sci Food Agric 2010, 90, 556–565.
Suproniene S, Justesen AF, Nicolaisen M, Mankeviciene A, Dabkevicius Z, Semaskiene R, Leistrumaite A: Distribution of trichothecene and zearalenone producing Fusarium species in grain of different cereal species and cultivars grown under organic farming conditions in Lithuania. Ann Agric Environ Med 2010, 17, 79–86.
Wiwart M, Kandler W, Perkowski J, Berthiller F, Preinerstorfer B, Suchowilska E, Buśko M, Laskowska M, Krska R: Concentrations of some metabolites produced by fungi of the genus Fusarium and selected elements in spring spelt grain. Cereal Chem 2009, 86, 52–60.
Xu X, Nicholson P: Community ecology of fungal pathogens causing wheat head blight. Ann Rev Phytopathol 2009, 47, 83–103.
Young JC: Microwave-assisted extraction of the fungal metabolite ergosterol and total fatty-acids. J Agric Food Chem 1995, 6, 43–49.
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