BRIEF COMMUNICATION
Changes of cytotoxicity of Ti-6Al-4V alloy made by DMLS technology as effect of the shot peening
1
Department of General Burgery, St. John of Dukla Centre Oncology for Lublin Region, Poland
2
Department of Materials Engineering, Faculty of Mechanical Engineering, Lublin University of Technology, Lublin, Poland
3
Faculty of Human Sciences, University of Economics and Innovation, Lublin, Poland
Corresponding author
Ann Agric Environ Med. 2020;27(4):706-712
KEYWORDS
TOPICS
ABSTRACT
Objective:
The aim the study was to investigate the impact of the shot peening process on the condition of the surface layer and biological properties of titanium alloy produced by means of the Direct Metal Laser Sintering (DMLS) method.
Material and Methods:
Specimens were prepared by the EOSINT M280 metal sintering laser system. The surfaces were subjected to the shot peening process using three different media, i.e. steel shot, nutshell granules and ceramic beads, after which they were subjected to profilometric analysis, scanning electron microscopic (SEM) observations and energy dispersive X-ray spectroscopy (EDS) tests, as well as to assessment of biological compatibility in terms of cytotoxicity (SH-SY5Y cell lines).
Results:
The general results obtained from the tests indicate satisfactory biocompatibility of the examined surfaces and that the impact of the shot peening process on the titanium alloy cytotoxicity is acceptable.
Conclusions:
The lowest cytotoxicity was demonstrated by the surfaces modified by ceramic beads than the nutshells and the biggest steel shot correspondingly. Moreover, the shot peening process carried out by means of CrNi steel and ceramic shot caused the reduction of surface roughness when, for the surface processing by means of nutshell granules, the increase of surface roughness was observed compared with the unmodified surface of titanium alloy samples.
The aim the study was to investigate the impact of the shot peening process on the condition of the surface layer and biological properties of titanium alloy produced by means of the Direct Metal Laser Sintering (DMLS) method.
Material and Methods:
Specimens were prepared by the EOSINT M280 metal sintering laser system. The surfaces were subjected to the shot peening process using three different media, i.e. steel shot, nutshell granules and ceramic beads, after which they were subjected to profilometric analysis, scanning electron microscopic (SEM) observations and energy dispersive X-ray spectroscopy (EDS) tests, as well as to assessment of biological compatibility in terms of cytotoxicity (SH-SY5Y cell lines).
Results:
The general results obtained from the tests indicate satisfactory biocompatibility of the examined surfaces and that the impact of the shot peening process on the titanium alloy cytotoxicity is acceptable.
Conclusions:
The lowest cytotoxicity was demonstrated by the surfaces modified by ceramic beads than the nutshells and the biggest steel shot correspondingly. Moreover, the shot peening process carried out by means of CrNi steel and ceramic shot caused the reduction of surface roughness when, for the surface processing by means of nutshell granules, the increase of surface roughness was observed compared with the unmodified surface of titanium alloy samples.
Żebrowski R, Walczak M, Drozd K, Jarosz MJ. Changes of cytotoxicity of Ti-6Al-4V alloy made by DMLS technology as effect of the shot
peening. Ann Agric Environ Med. 2020; 27(4): 706–712. doi: 10.26444/aaem/116386
REFERENCES (48)
1.
Walczak M. Influence of the selected technological processes on durability of metal-ceramic systems used in dental prosthetics. Lublin University of Technology Publishers, 2014.
2.
Elshahawy W, Watanabe I. Biocompatibility of dental alloys used in fixed prosthodontics. Tanta Dental J. 2014; 11(2): 150–159. https://doi.org/10.1016/j.tdj.....
3.
El-Hadad S, Safwat EM, Sharaf NF. In-vitro and in-vivo, cytotoxicity evaluation of cast functionally graded biomaterials for dental implantology. Mater Sci Eng C Mater Biol Appl. 2018; 93(1): 987–995. https://doi.org/10.1016/j.msec....
4.
Jelliti S, Richard C, Retraint D, Roland T, Chemkhi M, Demangel C. Effect of surface nanocrystallization on the corrosion behavior of Ti6Al-4V titanium alloy. Surf Coat Technol. 2013; 224: 82–87. https://doi.org/10.1016/j.surf....
5.
Ahmed AA, Mhaede M, Wollmann M, Wagner L. Effect of micro shot peening on the mechanical properties and corrosion behavior of two microstructure Ti-6Al-4V alloy. Appl Surf Sci. 2016; 363: 50–58. https://doi.org/10.1016/j.apsu....
6.
Mikami T, Miyata K, Komatsu K, Yamashita K, Wanibuchia M, Mikuni N. Exposure of titanium implants after cranioplasty: A matter of long-term consequences. Interdisciplinary Neurosurgery: Adv Tech Case Man. 2017; 8: 64–67. https://doi.org/10.1016/j.inat....
7.
Harun WSW, Manam NS, Kamariah MSIN, et al. A review of powdered additive manufacturing techniques for Ti-6Al-4V biomedical applications. Powder Techn. 2018; 331: 74–97. https://doi.org/10.1016/j.powt....
8.
Javaid M, Haleem A. Current status and challenges of Additive manufacturing in orthopaedics: An overview. J Clin Orthop Trauma. 2019; 10(2): 380–386. https://doi.org/10.1016/j.jcot....
9.
Bose S, Ke D, Sahasrabudhe H, Bandyopadhyay A. Additive manufacturing of biomaterials. Progr Mater Sci. 2018; 93: 45–111. https://doi.org/10.1016/j.pmat....
10.
Mutua J, Nakata S, Onda T, Chen Z-Ch. Optimization of selective laser melting parameters and influence of post heat treatment on microstructure and mechanical properties of maraging steel. Mater Design. 2018; 139: 486–497. https://doi.org/10.1016/j.matd....
11.
Molaei R, Fatemi A. Fatigue Design with Additive Manufactured Metals: Issues to Consider and Perspective for Future Research. Proced Eng. 2018; 213: 5–16. https://doi.org/10.1016/j.proe....
12.
Szewczenko J, Walke W, Nowinska K, Marciniak J, Corrosion resistance of Ti-6Al-4V alloy after diverse surface treatments, Materialwissenschaft und Werkstofftechnik. 2010; 41(5): 360–371. https://doi.org/10.1002/mawe.2....
13.
Vaithilingam J, Prina E, Goodridge RD, Hague RJM, Edmondson S, Rose FRAJ, et al. Surface chemistry of Ti6Al4V components fabricated using selective laser melting for biomedical applications, Mater Sci Eng C Mater Biol Appl. 2016; 67: 294–303. https://doi.org/10.1016/j.msec....
14.
Murali R, Bonar F, Kirsh G, Walter WK, Walter WL. Osteolysis in Third-Generation Alumina Ceramic-on-Ceramic Hip Bearings With Severe Impingement and Titanium Metallosis. J Arthroplasty 2008; 23(8): 13–19. https://doi.org/10.1016/j.arth....
15.
Lukina E, Laka A, Kollerov M, Sampiev M, Mason P, Wagstaff P, et al. Metal concentrations in the blood and tissues after implantation of titanium growth guidance sliding instrumentation. Spine J. 2016; 16(3): 380–388. https://doi.org/10.1016/j.spin....
16.
Hart A, Sabah S, Bandi A, Maggiore P, Tarassoli P, Sampson B, et al. Sensitivity and specificity of blood cobalt and chromium metal ions for predicting failure of metal-on-metal hip replacement. J Bone Joint Surg Br. 2011; 93(10): 1308–1313. https://doi.org/10.1302/0301–6....
17.
Jacobs JJ, Silverton C, Hallab NJ. Metal release and excretion from cement less titanium alloy total knee replacements. Clin Orthop Relat Res. 1999; 358: 173–180.
18.
Cundy T, Antoniou G, Sutherland L, Cundy P. Serum titanium, niobium, and aluminum levels after instrumented spinal arthrodesis in children. Spine. 2013; 38(7): 564–570. https://doi.org/10.1097/BRS.0b....
19.
Kasai Y, Iida R, Uchida A. Metal concentrations in the serum and hair of patients with titanium alloy spinal implants. Spine. 2003; 28(12): 1320–1326. https://doi.org/10.1097/01.BRS....
20.
Richardson TD, Pineda SJ, Strenge KB, Van Fleet TA, MacGregor M, Milbrandt JC, et al. Serum titanium levels after instrumented spinal arthrodesis. Spine. 2008; 33(7): 792–796. https://doi.org/10.1097/BRS.0b....
21.
Wang JC, Warren D, Harvinder S, Harvinder S., Foster B, Sunita B, et al. Metal debris from titanium spinal implants. Spine. 1999; 24(9): 899–903.
22.
Castner DG, Ratner BD, Biomedical surface science: foundations to frontiers, Surf Sci. 2002; 500(1–3): 28–60. https://doi.org/10.1016/S0039–....
23.
Paital SR, Dahotre NB. Calcium phosphate coatings for bio-implant applications: materials, performance factors, and methodologies, Mater Sci Eng R Rep. 2009; 66(1–3): 1–70. https://doi.org/10.1016/j.mser....
24.
Santavirta S, Bohler M, Harris W, Konttinen YT, Lappalainen R, Muratoglu O, et al. Alternative materials to improve total hip replacement tribology. Acta Orthop Scand 2003; 74(4): 380–388.
25.
Tipper J, Hatton A, Nevelos J, Ingham E, Doyle C, Streicher R, et al. Alumina-alumina artificial hip joints: Part II. Characterization of the wear debris from in vitro hip joint simulations. Biomaterials 2002; 23(16): 3441–3448. https://doi.org/10.1016/S0142–....
26.
Żebrowski R, Walczak M, Effect of the shop peening on surface properties and tribological performance of Ti-6Al-4V alloy produced by means of DMLS technology, Arch Metall Mater. 2019; 64(1): 377–386. https://doi.org/10.24425/amm.2....
27.
Dai S, Zhu Y, Huang Z, Microstructure evolution and strengthening mechanisms of pure titanium with nano-structured surface obtained by high energy shot peening. Vacuum. 2016; 125: 215–221. https://doi.org/10.1016/j.vacu....
28.
Żebrowski R, Walczak M, Klepka T, Pasierbiewicz K, Effect of the shot peening on surface properties of Ti-6Al-4V alloy produced by means of DMLS technology. Eksploat i Niezawodnosc – Maintenance and Reliability. 2019; 21(1): 46–53. http://dx.doi.org/10.17531/ein....
29.
Żebrowski R, Walczak M. The effect of shot peening on the corrosion behaviour of Ti-6Al-4V alloy made by DMLS. Adv Mat Sci. 2018; 18(3): 43–54. https://doi.org/10.1515/adms-2....
30.
Wataha JC. Biocompatibility of dental casting alloys: a review. J Prosthet Dent. 2000; 83(2): 223–234. https://doi.org/10.1016/S0022–....
31.
Al-Hiyasat AS, Darmani H. The effects of recasting on the cytotoxicity of base metal alloys. J Prosthet Dent. 2005; 93(2): 158–163. https://doi.org/10.1016/j.pros....
32.
Pallavicini P, Cabrini E, Cavallaro G, Chirico G, Collini M, D’Alfonso L, et al. A comparative study of in-vitro biocompatibility and of their interaction with SH-SY5Y neuroblastoma cells. J Inorg Biochem. 2015; 151: 123–31. doi: 10.1016/j.jinorgbio.2015.05.002.
33.
Ciofani G, Raffa V, Vittorio O, Cuschieri A, Pizzorusso T, Costa M, Bardi G. In vitro and in vivo biocompatibility testing of functionalized carbon nanotubes. Methods Mol Biol. 2010; 625: 67–83. doi: 10.1007/978–1–60761–579–8_7.
34.
Teppola H, Sarkanen JR, Jalonen TO, Linne ML. Morphological Differentiation Towards Neuronal Phenotype of SH-SY5Y Neuroblastoma Cells by Estradiol, Retinoic Acid and Cholesterol. Neurochem Res. 2016; 41(4): 731–747. https://doi.org/10.1007/s11064....
35.
Liu YG, Li MQ, Liu HJ. Nanostructure and surface roughness in the processed surface layer of Ti-6Al-4V via shot peening. Mater Charact. 2017; 123: 83–90. https://doi.org/10.1016/j.matc....
36.
Ganesh BKC, Sha W, Ramanaiah N, Krishnaiah A. Effect of shot peening on sliding wear and tensile behavior of titanium implant alloys. Mater Design. 2014; 56: 480–486. https://doi.org/10.1016/j.matd....
37.
Tąta A., Szkudlarek A, Pacek J, Molenda M, Proniewicz E. Peptides of human body fluids as sensors of corrosion of titanium to titanium dioxide. SERS application. Appl Surf Sci. 2019; 473: 107–120. https://doi.org/10.1016/j.apsu....
38.
Benedetti M, Torresani E, Leoni M, Fontanari V, Bandini M, Pederzolli C, et al. The effect of post-sintering treatments on the fatigue and biological behavior of Ti-6Al-4V ELI parts made by selective laser melting. J Mech Behav Biomed Mater. 2017; 71: 295–306. https://doi.org/10.1016/j.jmbb....
39.
Unal O, Karaoglanli AC, Varol R, Kobayashi A. Microstructure evolution and mechanical behavior of severe shot peened commercially pure titanium. Vacuum. 2014; 110: 202–206. https://doi.org/10.1016/j.vacu....
40.
Kameyama Y, Komotori J. Effect of micro ploughing during fine particle peening process on the microstructure of metallic materials. J Mater Process Tech. 2009; 209(20): 6146–6155. https://doi.org/10.1016/j.jmat....
41.
Aparicio C, Gil FJ, Fonseca C, Barbosa M, Planell JA. Corrosion behaviour of commercially pure titanium shot blasted with different materials and sizes of shot particles for dental implant applications. Biomaterials 2003; 24(2): 263–273. https://doi.org/10.1016/S0142–....
42.
Velasco-Ortega E, Alfonso-Rodríguez CA, Monsalve-Guil L, et al. Relevant aspects in the surface properties in titanium dental implants for the cellular viability. Mater Sci Eng C Mater Biol Appl. 2016; 64: 1–10. https://doi.org/10.1016/j.msec....
43.
Kim WG, Ahn KH, Jeong YH, Vang MS, Choe HC. Surface Characteristics and Cell Proliferation of Mechanical Sandblasted Ti-30Ta-xNb Surface. Proced Engin. 2011; 10: 2399–2404. https://doi.org/10.1016/j.proe....
44.
Wang H-Y, Zhu R-F, Lu Y-P, et al. Preparation and properties of plasma electrolytic oxidation coating on sandblasted pure titanium by a combination treatment. Mater Sci Eng C Mater Biol Appl. 2014; 42(1): 657–664. https://doi.org/10.1016/j.msec....
45.
Wei D, Du Q, Guo S, et al. Structures, bonding strength and in vitro bioactivity and cytotoxicity of electrochemically deposited bioactive nano-brushite coating/TiO2 nanotubes composited films on titanium. Surf Coat Technol. 2018; 340: 93–102. https://doi.org/10.1016/j.surf....
46.
Lee EM, Smith K, Gall K, Boyan BD, Schwartz Z. Change in surface roughness by dynamic shape-memory acrylate networks enhances osteoblast differentiation. Biomaterials 2016; 110: 34–44. https://doi.org/10.1016/j.biom....
47.
Havlikova J, Strasky J, Vandrovcova M, et al. Innovative surface modification of Ti-6Al-4V alloy with a positive effect on osteoblast proliferation and fatigue performance. Mater Sci Eng C Mater Biol Appl. 2014; 39: 371–379. https://doi.org/10.1016/j.msec....
48.
Tuomi JT, Björkstrand RV, Pernu ML, Salmi MVJ, Huotilainen EI, Wolff JEH, et al. In vitro cytotoxicity and surface topography evaluation of additive manufacturing titanium implant materials. J Mater Sci: Mater Med. 2017; 28(3): 53. https://doi.org/10.1007/s10856....
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