RESEARCH PAPER
A study of Neospora caninum and Toxoplasma gondii antibody seroprevalence in healthy cattle in the Czech Republic
| 1 | Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic |
| 2 | Department of Virology and Serology, State Veterinary Institute, Prague, Czech Republic |
| 3 | Department of Mathematics and Statistics, Faculty of Science, Masaryk University, Brno, Czech Republic |
Ann Agric Environ Med. 2015;22(1):32–34
KEYWORDS
ABSTRACT
Introduction and objective:
The aim of study was to test the sera of healthy dairy cows by ELISAs, the methods also used in other groups of animals in the Czech Republic, and thus to obtain actual data about N. caninum and T. gondii seroprevalence in cattle.
Material and Methods:
In the Czech Republic, sera from 546 clinically healthy dairy cows (Bos primigenius f. taurus) aged > 2 years from 49 farms in 7 districts were collected. Sera were tested for Neospora caninum antibodies by a commercial competitive-inhibition enzyme-linked immunosorbent assay; samples with more than 30% inhibition were considered as positive. The same samples were also analysed for Toxoplasma-specific IgG antibodies by an enzyme-linked immunosorbent assay; samples with more than 50% S/P were considered as positive.
Results:
Antibodies against N. caninum were found only in 3 cows (0.5%) with inhibitions of 47, 78 and 85. Antibodies against T. gondii were found in 53 cows (9.7%) with S/P ranging from 51% to over 211%; positive animals were found in 4 of 7 districts, with prevalences ranging from 8% – 14%. Indication of mixed infections (concurrent presence of both N. caninum and T. gondii antibodies) was not proved.
Conclusions:
The results of the study indicate that dairy cows in the Czech Republic have a relatively low seroprevalence for both N. caninum and T. gondii. Therefore, natural infection with N. caninum and T. gondii seems not to be very common in Czech cattle. These results show actual data about N. caninum and T. gondii infection in healthy dairy cattle from the Czech Republic.
The aim of study was to test the sera of healthy dairy cows by ELISAs, the methods also used in other groups of animals in the Czech Republic, and thus to obtain actual data about N. caninum and T. gondii seroprevalence in cattle.
Material and Methods:
In the Czech Republic, sera from 546 clinically healthy dairy cows (Bos primigenius f. taurus) aged > 2 years from 49 farms in 7 districts were collected. Sera were tested for Neospora caninum antibodies by a commercial competitive-inhibition enzyme-linked immunosorbent assay; samples with more than 30% inhibition were considered as positive. The same samples were also analysed for Toxoplasma-specific IgG antibodies by an enzyme-linked immunosorbent assay; samples with more than 50% S/P were considered as positive.
Results:
Antibodies against N. caninum were found only in 3 cows (0.5%) with inhibitions of 47, 78 and 85. Antibodies against T. gondii were found in 53 cows (9.7%) with S/P ranging from 51% to over 211%; positive animals were found in 4 of 7 districts, with prevalences ranging from 8% – 14%. Indication of mixed infections (concurrent presence of both N. caninum and T. gondii antibodies) was not proved.
Conclusions:
The results of the study indicate that dairy cows in the Czech Republic have a relatively low seroprevalence for both N. caninum and T. gondii. Therefore, natural infection with N. caninum and T. gondii seems not to be very common in Czech cattle. These results show actual data about N. caninum and T. gondii infection in healthy dairy cattle from the Czech Republic.
REFERENCES (20)
1.
Dubey JP, Schares G. Neosporosis in animals-the last five years. Vet Parasitol. 2011; 180: 90–108.
2.
Hurkova L, Halova D, Modry D. The prevalence of Neospora caninum antibodies in bulk milk of dairy herds in the Czech Republic: a case report. Vet Med (Prague). 2005; 50: 549–552.
3.
Vaclavek P, Koudela B, Modry D, Sedlak K. Seroprevalence of Neospora caninum in aborting dairy cattle in the Czech Republic. Vet Parasitol. 2003; 115: 239–245.
4.
Havlik O, Hubner J. Serological survey of toxoplasmosis in some domestic and wildlife animals. Czech Epidem Microb Imunol. 1958; 7: 396–402.
5.
Kozojed V, Roudna V, Jira J, Hodkova Z. Surveillance of toxoplasmosis in animal hosts (in Czech). Zprávy Československé Zoologické Společnosti. 1977; 52: 10–12.
6.
STATISTICA: StatSoft, Inc. (2011). STATISTICA (data analysis software system), version 10, www.statsoft.com (access: 2013.04.15).
7.
Kul O, Kabakci N, Yildiz K, Ocal N, Kalender H, Ilkme NA. Neospora caninum associated with wpidemic abortions in dairy cattle: The first clinical neosporosis report in Turkey. Vet Parasitol. 2009; 159: 69–72.
8.
Loobuyck M, Frossling J, Lindberg A, Bjorkman C. Seroprevalence and spatial distribution of Neospora caninum in a population of beef cattle. Prev Vet Med. 2009; 92: 116–122.
9.
Eiras C, Arnaiz I, Alvarez-García G, Ortega-Mora LM, Sanjuanl ML, Yus E, Dieguez FJ. Neospora caninum seroprevalence in dairy and beef cattle from the northwest region of Spain, Galicia. Prev Vet Med. 2011; 98: 128–132.
10.
Reiterova K, Spilovska S, Antolova D, Dubinsky P. Neospora caninum, potential cause of abortions in dairy cows: The current serological follow-up in Slovakia. Vet Parasitol. 2009; 159: 1–6.
11.
Gottstein B, Hentrich B, Thur B, Busato A, Stark KDC, Muller N. Molecular and immunodiagnostic investigations on bovine neosporosis in Switzerland. Int J Parasitol. 1998; 28: 679–691.
12.
Knapen F, Franchimont JH, Lugt G. Prevalence of antibodies to toxoplasma in farm animals in the Netherlands and its implication for meat inspection. Vet Quarter. 1982; 4: 101–105.
13.
Panadero R, Painceira A, Lopez C, Vazquez L, Paz A, Diaz P, Dacal V, Cienfuegos S, Fernandez G, Lago N, Diez-Banos P, Morrondo P. Seroprevalence of Toxoplasma gondii and Neospora caninum in wild and domestic ruminants sharing pastures in Galicia (Northwest Spain). Res Vet Sci. 2010; 88: 111–115.
14.
Yildiz K, Kul O, Babur C, Kilic S, Gazyagci AN, Celebi B, Gurcan IS: Seroprevalence of Neospora caninum in dairy cattle ranches with high abortion rate: Special emphasis to serologic co-existence with Toxoplasma gondii, Brucella abortus and Listeria monocytogenes. Vet Parasitol. 2009; 164: 306–310.
15.
Gondim LFP, Sartor IF, Hasegawa M, Yamane I. Seroprevalence of Neospora caninum in dairy cattle in Bahia, Brazil. Vet Parasitol. 1999; 86: 71–75.
16.
Bartova E, Sedlak K. Toxoplasma gondii and Neospora caninum antibodies in goats in the Czech Republic. Vet Med (Prague). 2012; 57(3): 111–114.
17.
Bartova E, Sedlak K. Seroprevalence of Toxoplasma gondii and Neospora caninum in slaughtered pigs in the Czech Republic. Parasitol. 2011; 138: 1369–1371.
18.
Bartova E, Sedlak K, Literak I. Presence of Toxoplasma gondii and Neospora caninum antibodies in sheep in the Czech Republic. Vet Parasitol. 2009; 161: 131–132.
19.
Bartova E, Sedlak K, Syrova M, Literak I. Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic. Parasitol Res. 2010; 107: 783–785.
20.
Bartova E, Sedlak K, Pavlik I, Literak I. Prevalence of Neospora caninum and Toxoplasma gondii antibodies in wild ruminants from the countryside or captivity in the Czech Republic. J Parasitol. 2007; 93: 1216–1218.
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