RESEARCH PAPER
Babesia canis and tick-borne encephalitis virus (TBEV) co-infection in a sled dog
| 1 | Department of Parasitology, Institute of Zoology, Faculty of Biology, University of Warsaw, Poland |
| 2 | Department of Pathology and Veterinary Diagnostics, University of Life Sciences – SGGW Warsaw, Poland |
Ann Agric Environ Med. 2013;20(3):426–430
KEYWORDS
ABSTRACT
Introduction and objectives:
Sporting dogs, including sled dogs, are particularly prone to tick-borne infection either due to training/racing in forest areas or through visits to endemic areas. The aim was to present tick-borne infections in a 6-dog racing team after a race in Estonia.
Material and Methods:
On the 4th day after return to Poland, the first dog presented with babesiosis symptoms and was diagnosed and treated accordingly. Next morning, the dog showed neurological symptoms and was diagnosed with tick-borne encephalitis (TBE). Diagnosis was confirmed by a high level of IgG antibodies (922 IU/ml), detected in serum 3 months later. The second dog presented with babesiosis symptoms on the 7th day after return. Babesia DNA was extracted from blood, amplified and sequenced to answer the question of whether the dogs became infected during the race in Estonia or in Poland.
Results and conclusions:
Sequencing of a fragment of Babesia 18S rDNA revealed that these two isolates were identical to one another and closely related to the B. canis sequence originally isolated from the dog and Dermacentor reticulatus ticks in Poland. Thus, this is the first confirmed case of B.canis and TBEV co-infection and first confirmed case of TBE in a dog in Poland.
Sporting dogs, including sled dogs, are particularly prone to tick-borne infection either due to training/racing in forest areas or through visits to endemic areas. The aim was to present tick-borne infections in a 6-dog racing team after a race in Estonia.
Material and Methods:
On the 4th day after return to Poland, the first dog presented with babesiosis symptoms and was diagnosed and treated accordingly. Next morning, the dog showed neurological symptoms and was diagnosed with tick-borne encephalitis (TBE). Diagnosis was confirmed by a high level of IgG antibodies (922 IU/ml), detected in serum 3 months later. The second dog presented with babesiosis symptoms on the 7th day after return. Babesia DNA was extracted from blood, amplified and sequenced to answer the question of whether the dogs became infected during the race in Estonia or in Poland.
Results and conclusions:
Sequencing of a fragment of Babesia 18S rDNA revealed that these two isolates were identical to one another and closely related to the B. canis sequence originally isolated from the dog and Dermacentor reticulatus ticks in Poland. Thus, this is the first confirmed case of B.canis and TBEV co-infection and first confirmed case of TBE in a dog in Poland.
REFERENCES (31)
1.
Welc-Faleciak R, Rodo A, Sinski E, Bajer A. Babesia canis and other tick-borne infections in dogs in Central Poland. Vet Parasitol. 2009; 166: 191–198.
2.
Dantas-Torres F. Canine vector-borne diseases in Brazil. Parasites & Vectors 2008; 1: 25, doi:10.1186/1756–3305–1–25.
3.
Matjila PT, Leisewitz AL, Jongejan F, Penzhorn BL. Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa. Vet Parasitol. 2008; 155: 152–157.
4.
Solano-Gallego L, Trotta M, Razia L, Furlanello T, Caldin M. Molecular survey of Ehrlichia canis and Anaplasma phagocytophilum from blood of dogs in Italy. Ann NY Acad Sci. 2006; 1078: 515–518.
5.
Bourdoiseau G. Canine babesiosis in France. Vet Parasitol. 2006; 138: 118–125. 6. Adaszek L, Winiarczyk S. Molecular characterization of Babesia canis canis isolates from naturally infected dogs in Poland. Vet Parasitol. 2008; 152: 235–241.
6.
Zygner W, Jaros S, Wedrychowicz H. Prevalence of Babesia canis, Borrelia afzelii, and Anaplasma phagocytophilum infection in hard ticks removed from dogs in Warsaw (central Poland). Vet Parasitol. 2008; 153: 139–142.
7.
Adaszek L. Wybrane aspekty epidemiologii babeszjozy, boreliozy i erlichiozy u psów. PhD thesis 2006, Faculty of Veterinary Medicine in Lublin, Poland.
8.
Czupryna P, Moniuszko A, Pancewicz SA, Grygorczuk S, Kondrusik M, Zajkowska J. Tick-borne encephalitis in Poland in years 1993–2008- epidemiology and clinical presentation. A retrospective study of 687 patients. Eur J Neurol. 2011; 18: 673–679.
9.
Kicman-Gawłowska A, Chrześcijańska I, Stefanoff P. [Meningitis and encephalitis in Poland in 2006]. Przegl Epidemiol. 2008; 62: 253–260 (in Polish).
10.
Suss J. Tick-borne encephalitis in Europe and beyond--the epidemiological situation as of 2007. Euro Surveillance 2008; 13: pii: 18916.
11.
Lindblad G. A case of tick-borne encephalitis in a dog. Medlernsblad Sveriges Veterinarforbund 1960; 12: 416–417.
12.
Pfeffer M, Dobler G. Tick-borne encephalitis virus in dogs- is this an issue? Parasites & Vectors 2011; 4: 59.
13.
Leschnik MW, Kirtz GC, Thalhammer JG. Tick-borne encephalitis (TBE) in dogs. Int J Med Microbiol. 2002; 291(Suppl. 33): 66–69.
14.
Kirtz G. FSME- Infektion in einer osterreichischen Hundepopulation. Diss Med Vet., Vien. 1999. 16. Reiner B, Fischer A. Fruhsommer-Meningoenzephalitis (FSME) beim Hund in Deutschland: zwei Fallberichte. Kleintierpraxis 1998; 43: 255–269.
15.
Tipold A, Fatzer R, Holzmann H. Zentraleuropaische Zeckenenzephalitis beim Hund. Kleintierpraxis 1993; 38: 619–628.
16.
Weissenbock H, Suchy A, Holzmann H. Tick-borne encephalitis in dogs: neuropathological findings and distribution of antigen. Acta Neuropathol. 1998; 95: 361–366.
17.
Zygner W. Choroby pasożytnicze przenoszone przez stawonogi zagrażające psom wyjeżdżającym do europejskich krajów Basenu Morza Śródziemnego i Portugalii. Część II. Babeszjoza, teilerioza i hepatozoonoza. Życie Weteryn. 2006; 81: 595–603 (in Polish).
18.
Bonnet S, Jouglin M, Malandrin L, Becker C, Agoulon A, L’Hostis M, Chauvin A. Transstadial and transovarial persistence of Babesia divergens DNA in Ixodes ricinus ticks fed on infected blood in a new skin-feeding technique. Parasitology 2007a; 134: 197–207.
19.
Bonnet S, Jouglin M, L’Hostis M, Chauvin A. Babesia sp. EU1 from roe deer and transmission within Ixodes ricinus. Emerg Infect Dis. 2007b; 13: 1208–1210.
20.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol Biol Evol. 2011; 28: 2731–2739.
21.
Stadtbaumer K, Leschnik MW, Nell B. Tick-borne encephalitis as a possible cause of optic neuritis in a dog. Vet Ophthalmol. 2004; 7: 271–277.
22.
Krause PJ, McKay K, Thompson CA, Sikand VK, Lentz R, Lepore T, Closter L, Christianson D, Telford SR, Persing D, Radolf JD, Spielman A. Deer-Associated Infection Study Group. Disease-specific diagnosis of coinfecting tickborne zoonoses: babesiosis, human granulocytic ehrlichiosis, and Lyme disease. Clin Infect Dis. 2002; 34: 1184–1191.
23.
Abrams Y. Complications of coinfection with Babesia and Lyme disease after splenectomy. J Am Board Fam Med. 2008; 21: 75–77.
24.
Zygner W, Gójska O, Rapacka G, Jaros D, Wędrychowicz H. Hematological changes during the course of canine babesiosis caused by large Babesia in domestic dogs in Warsaw (Poland). Vet Parasitol. 2007; 145: 146–151.
25.
Suss J, Schrader C, Abel U, Bormane A, Duks A, Kalnina V. Characterization of tick-borne encephalitis (TBE) foci in Germany and Latvia (1997–2000). Int J Med Microbiol. 2002; 291(Suppl. 33): 34–42.
26.
Zygner W, Wedrychowicz H. Occurrence of hard ticks in dogs from Warsaw area. Ann Agric Environ Med. 2006; 13: 355–359.
27.
Wójcik-Fatla A, Cisak E, Zając V, Zwoliński J, Dutkiewicz J. Prevalence of tick-borne encephalitis virus in Ixodes ricinus and Dermacentor reticulatus ticks collected from the Lublin region (eastern Poland). Ticks and Tick-borne Dis 2011; 2: 16–19.
28.
Dautel H, Dippel C, Oehme R, Hartelt K, Schettler E. Evidence for an increased geographical distribution of Dermacentor reticulatus in Germany and detection of Rickettsia sp. RpA4. Int J Med Microb. 2006; 296(S1): 149–156.
29.
Heile C., Heydorn AO, Schein E. Dermacentor reticulatus (Fabricius, 1794)- distribution, biology and vector for Babesia canis in Germany. Berlin Münch Tierärzt Wochenschrif 2006; 119: 330–334.
30.
Nijhof AM, Bodaan C, Postigo M, Nieuwenhujis H, Opsteegh M, Franssen L, Jebbnik F, Jongejan F. Tick and associated pathogens collected from domestic animals in the Netherlands. Vector Borne Zoonotic Dis. 2007; 7: 584–595.
31.
Sreter T, Szell Z, Varga I. Spatial distribution of Dermacentor reticulatus and Ixodes ricinus in Hungary: evidence for change? Vet Parasitol. 2005; 128: 347–351.
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