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RESEARCH PAPER
 
CC BY-NC-ND 3.0
 
 

Peroxidative metabolism of arachidonic acid in the course of Lyme arthritis

Anna Moniuszko 2  ,  
 
1
Department of Analytical Chemistry, Medical University of Bialystok, Poland
2
Department of Infectious Diseases and Neuroinfection, Medical University of Bialystok, Poland
Ann Agric Environ Med 2015;22(3):433–437
KEYWORDS:
ABSTRACT:
Objective:
The objective of the study was measurement of serum arachidonic acid level as well as the product of its peroxidation – 8-isoPGF, and the activity of phospholipase A2 and PAF-acetylhydrolase that participate in releasing 8-isoPGF from glycerol skeleton, and the potential designation of their role in the pathomechanism of Lyme disease (LD).

Material and Methods:
Changes in the phospholipid arachidonic acid level and the level of 8-isoPGF were determined in the plasma and urine of patients with LA (n=57) and of healthy controls (n=41). The activity of phospholipase A2 and PAF acetylhydrolase were assayed. All examined parameters were also measured in the plasma of some LA patients (n=13) after antibiotics treatment.

Results:
An almost 3-fold higher level of the total plasma 8-isoPGF was observed in LA patients compared to the controls, while in the urine it increased over 5-fold. The plasma PLA2 activity was more than 3-fold higher in LA patients than in the healthy subjects, while PAF acetylhydrolase activity was observed to be modestly, but not significantly lower. The total 8-isoPGF level in the plasma and urine of LA patients was significantly lower after antibiotics treatment. The plasma activity of PAF-AH in the LA patients was increased, while the cPLA2 activity decreased after antibiotics treatment.

Conclusions:
It may be suggested that in the course of LA, the level of binding 8-isoPGF is significantly enhanced, and it may also be suggested that uncontrolled changes in the lipid status of some patients may make their Lyme arthritis unresponsive to antibiotics.

CORRESPONDING AUTHOR:
Anna Moniuszko   
Department of Infectious Diseases and Neuroinfection, Medical University of Bialystok, Poland
 
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