In the twentieth century, fumigation became a very popular method of disinfection, although in the same century many agents used as fumigants were withdrawn for ecological reasons. Fogging (fumigation) is a relatively new disinfection technology using dry fog, which behaves more like a gas and easily fills the sanitized space, reaching all surfaces in the room. The undoubted advantage of fumigation is the possibility of disinfecting difficult to clean areas. Fumigation has become particularly important in the twenty-first century due to procedures related to combating and preventing the spread of the coronavirus that causes COVID-19.

The aim of this review article is to summarize the current state of knowledge in the field of fumigation on the basis of past results of original research, taking into account new trends and possibilities of its application.

Brief description of the state of knowledge:
Due to the fact that fumigation is safe for apparatus, equipment, and electronics, while simultaneously enabling the highest possible bactericidal and virucidal levels, this method is widely used in various areas, both medical and non-medical. Fogging technology is used in the medical, pharmaceutical, and food industries, as well as in transportation, for air fumigation or surface disinfection in closed spaces, such as hospital and laboratory rooms, incubators, refrigerators, ships, trucks, railway containers, and aircraft, to name only a few. The most common fumigants are hydrogen peroxide and peracetic acid, and their mechanism of action is related to their oxidizing properties.

Hydrogen peroxide and peracetic acid are highly effective and non-toxic fumigants that can be safely used for fogging laboratory and medical equipment, pharmaceutical facilities, hospital rooms, and animal breeding rooms.

We are grateful to Steven Snodgrass for editorial assistance. This work was supported by the Project entitled „National intersectoral doctoral studies at the Medical University of Bialystok” (POWR.03.02.00-00-I050/16) co-funded from European Union funds within the framework of European Social Fund as part of Knowledge Education Development 2014-2020 Operational Programme, through the grant no 01/MSD/2019.
Krishnan J, Fey G, Stansfield C. Evaluation of a Dry Fogging System for Laboratory Decontamination. Appl Biosafety. 2012; 17(3): 132–141.
Horn HMSc, Niemeyer B. Aerosol disinfection of bacterial spores by peracetic acid on antibacterial surfaces and other technical materials. Am J of Inf Conrtol. 2020; 48: 1200–1203.
Boyce JM. Modern technologies for improving cleaning and disinfection of environmental surfaces in hospitals. Antimicrob Resist Infect Control. 2016; 5: 10.
Shin HJ, Kim H, Beuchat LR, Ryu J-H. Antimicrobial activities of organic acid vapors against Acidovorax citrulli, Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes on Cucurbitaceae seeds. Food Microbiol. 2020; 19: 103569.
Humayun T, Quresshi A, Roweily SFA, et al. Efficacy of hydrogen peroxide fumigation in improving disinfection of hospital rooms and reducing the number of microorgasims. J Ayub Med Coll Abbottabad. 2019; 31(4 Suppl 1)(4): 646–650. PMID: 31965767.
Quan JH, Ju LY, Bei S, et al. Evaluation of Vaporized Hydrogen Peroxide Fumigation as a Method for the Bio-decontamination of the High Efficiency Particulate Air Filter Unit. Biomed Environ Sci. 2013; 26(2): 110-117.
Oyeyemi A, Adesina A, Ogoina D. Fumigation of Schools for COVID-19 Prevention in Nigeria: The Need for a Rethink. Am J Trop Med Hyg. 2020; 103 (4): 1370–1371.
Amaeze NJ, Sharef MU, Henriquez FL, et al. Influence of delivery system on the efficacy of low concentrations of hydrogen peroxide in the disinfection of common healthcare-associated infection pathogens. Journal of Hospital Infection. 2020; 106: 189–195.
Bentley K, Dove BK, Parks SR, et al. Hydrogen peroxide vapour decontamination of surfaces artificially contaminated with norovirus surrogate feline calicivirus. J Hospital Infect. 2012; 80: 116–121.
Goyal SM, Chander Y, Yezli S, Otter JA. Evaluating the virucidal efficacy of hydrogen peroxide vapour. J Hospital Inf. 2014; 86: 255–259.
Rutala WA, Gergen MF, Sickbert-Bennett EE, et al. Effectiveness of improved hydrogen peroxide in decontaminating privacy curtains contaminated with multidrug-resistant pathogens. Am J Inf Control. 2014; 42: 426–428.
Wallace RL, Quellette M, Jean J. Effect of UV-C light or hydrogen peroxide wipes on the inactivation of methicillin-resistant Staphylococcus aureus, Clostridium difficile spores and norovirus surrogate. J Appl Microbiol. 2019; 127 (2): 586–597.
Kenters N, Gottlieb T, Hopman J, et al. An international survey of cleaning and disinfection practices in the healthcare environment. J Hospital Inf. 2018; 236–241.
Berrie E, Andrews L, Yezli S, Otter JA. Hydrogen peroxide vapour inactivation of adenovirus. Lett Appl Microbiol. 2011; 52; 555–558.
Kindermann J, Karbiener M, Leydold SM, et al. Virus disinfection for biotechnology applications: Different effectiveness on surface versus in suspension. Biologicals. 2020; 64: 1–9.
Pottage T, Lewis S, Lansley A, et al. Hazard Group 3 agent decontamination using hydrogen peroxide vapour in a class III microbiological safety cabinet. J Appl Microbiol. 2020; 128(1): 116–123.
Rogers JV, Choi YW. Inactivation of Francisella tularensis Schu S4 in a Biological Safety Cabinet Using Hydrogen Peroxide Fumigation. Appl Biosafety. 2008; 13(1): 15–20.
Mickelse RL, Wood J, Calfee MW, et al. Low-concentration hydrogen peroxide decontamination for Bacillus spore contamination in buildings. Remediation J. 2019; 30(1): 47–56.
Stuart J, Chewins J, Tearle J. Comparing the efficacy of formaldehyde with hydrogen peroxide fumigation on infectious bronchitis virus. Appl Biosafety. 2020; 25(2): 83–89.
Achmed R, Mulder R. A Systematic Review on the Efficacy of Vaporized Hydrogen Peroxide as a Non-Contact Decontamination System for Pathogens Associated with the Dental Environment. Int J Environ Res Public Health. 2021; 18,4748.
Knight GC, Craven HM. A model system for evaluating surface disinfection in dairy factory environments. Int J Food Microbiol. 2010; 137: 161–167.
Bore E, Langsrud S. Characterization of micro-organisms isolated from dairy industry after cleaning and fogging disinfection with alkyl amine and peracetic acid. J Appl Microbiol. 2005; 98: 96–105.
Ochowiak M, Krupińska A, Włodarczyk S, Matuszak M, Woziwodzki S, Szulc T. Analysis of the possibility of disinfecting surfaces using portable foggers in the era of the SARS-CoV-2 epidemic. Energies. 2021; 14(7): 2019.
Alawlaqi MM, Alarbi Askaa A. Impact of Acetic Acid on controlling Tomato Fruit Decay. Life Sci J. 2014; 11(3s): 114–119.
Velde FV, Grace MH, Pirovani ME, Lila MA. Impact of a new postharvest disinfection method based on peracetic acid fogging on the phenolic profile of strawberries. Postharvest Biol Technol. 2016; 117: 197–205.
Ríos-Castillo AG, González-Rivas F, Rodríguez-Jerez J. Bactericidal Efficacy of Hydrogen Peroxide-Based Disinfectants Against Gram-Positive and Gram-Negative Bacteria on Stainless Steel Surfaces. J Food Sci. 2017; 82(10): 2351–2356.
Celebi O, Buyuk F, Pottage T, et al. The use of germinants to potentiate the sensitivity of Bacillus anthracis spores to peracetic acid. Front Microbiol. 2016; 29; 7:18.
Richter WR, Wood JP, Wendling MQS, Rogers J. Inactivation of Bacillus anthracis spores to decontaminate subway railcar and related materials via the fogging of peracetic acid and hydrogen peroxide sporicidal liquids. J Environ Manage. 2017; 206: 800–806.
Wood JP, Calfee MW, Clayton M, et al. Evaluation of peracetic acid fog for the inactivation of Bacillus anthracis spore surrogates in a large decontamination chamber. J Hazardous Materials. 2013; 250–251: 61–67.
Malik DJ. Effect on biocidal efficacy of hydrogen peroxide vapour by catalase activity of nosocomial bacteria. J Hospital Infect. 2013; 83(4): 353–354.
World Heaith Organization. Coronavirus disease (COVID-19). Situation Report – 115. 2020.
World Heaith Organization. Cleaning and disinfection of environmental surfaces in the context of COVID-19. WHO/2019-nCoV/Disinfection/2020.
Kumira T, Yahata H, Uchiyama Y. Examination of material compatibilities with ionized and vaporized hydrogen peroxide decontamination. J Am Associat Lab Animal Sci. 2020; 59 (6): 703–711.
Beswick AJ, Farrant J, Makison C, et al. Comparison of Multiple Systems for Laboratory Whole Room Fumigation. Appl Biosaf. 2011; 3: 139–157.
Costa SAS, Paula OFP, Silva CRC, et al. Stability of antimicrobial activity of peracetic acid solutions used in the final disinfection process. Braz Oral Res. 2015; 29(1): 1–6.
Nielsen GD, Larsen ST, Wolkoff P. Re -evaluation of the WHO (2010) formaldehyde indoor air quality guideline for cancer risk assessment. Arch Toxicol. 2017; 91: 35–61.
Denby KJ, Iwig J, Bisson C, et al. The mechanism of a formaldehydesensing transcriptional regulator. Sci Rep. 2016; 9;6: 38879.
Alfa MJ, Lo E, Olson N, et al. Use of a daily disinfectant cleaner instead of a daily cleaner reduced hospital-acquired infection rates. Am J Inf Control. 2015; 43: 141–146.
Yang X, Liu Y-B. Nitric oxide fumigation for postharvest pest control on lettuce. Pest Manag Sci. 2019; 75: 390–395.
Hao l, Wu J, Zhang E, et al. Disinfection efficiency of positive pressure respiratory protective hood using fumigation sterilization cabinet. Biosafety and Health. 2019.
Vannier M, Chewins J. Hydrogen peroxide vapour is an effective replacement for Formaldehyde in a BSL4 Foot and mouth disease vaccine manufacturing facility. Letters in Applied Microbiol. 2019; 69: 237–245.
Goel S, Hawi S, Goel G, et al. Resilient and agile engineering solutions to address societal challenges such as coronavirus pandemic. Mat Today Chemistry. 2020; 17: 100300.