Blastocystis is one of the most frequently detected protozoa in the human large intestine. One of the most effective and cheap methods for detecting Blastocystis in faeces is culture on a special medium in anaerobic conditions. Sampling faeces using traditional containers and their transport to the laboratory has certain limitations: a sample taken in this way should reach the laboratory relatively quickly, moreover, some patients are uncomfortable during sampling and protection of material in this way. We propose utilizing a swab for sampling and transportation of the faeces samples to be examined for Blastocystis instead of using traditional containers. We believe this is an excellent method allowing the material to be transported over longer distances without additional, and sometimes expensive, safety measures, and at the same time permitting the possibility of obtaining living cells after a relatively long period of storage.
Andersen LO, Stensvold CR. Blastocystis in health and disease: are we moving from a clinical to a public health perspective? J Clin Microbiol. 2016; 54(3): 524–528. doi: 10.1128/JCM.02520-15.
Rudzińska M, Kowalewska B, Szostakowska B, Grzybek M, Sikorska K, Świątalska A. First report on the occurrence and subtypes of Blastocystis in pigs in Poland using sequence-tagged-site PCR and barcode region sequencing. Pathogens. 2020; 9(7): 595. doi: 10.3390/pathogens9070595.
Can H, Köseoğlu AE, Erkunt Alak S, et al. Molecular prevalence and subtyping of Blastocystis sp. isolates in stray cats of İzmir, Turkey: first report of “ST4 allele 42” in cats. Pol J Vet Sci. 2021; 24(2): 217–223. doi: 10.24425/pjvs.2021.137656.
Skotarczak B. Genetic diversity and pathogenicity of Blastocystis. Ann Agric Environ Med. 2018; 25(3): 411–416. doi: 10.26444/aaem/81315.
Stensvold C. Laboratory diagnosis of Blastocystis spp. Trop Parasitol. 2015; 5(1): 3–5. doi: 10.4103/2229-5070.149885.
Vaisusuk K, Saijuntha W, Sedlak S, et  al. Blastocystis subtypes detected in long-tailed macaques in Thailand – further evidence of cryptic host specificity. Acta Trop. 2018; 184: 78–82. doi:10.1016/j.actatropica.2017.09.002.
Amies CR. A modified formula for the preparation of Stuart’s transport medium. Can J Public Health. 1967; 58(7): 296–300.
Jones WR. The experimental infection of rats with Entamoeba histolytica; with a method for evaluating the anti-amoebic properties of new compounds. Ann Trop Med Parasitol. 1946; 40(2): 130–140. doi: 10.1080/00034983.1946.11685270.