HPLC analysis of potentially harmful substances released from dental filing aterials available on the EU market
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Department of Orthodontics, Medical University of Warsaw, Poland
College of Public Health, Zielona Góra, Poland
Institute of Chemistry, Siedlce University of Natural Sciences and Humanities, Poland
Private Practice, Krakow, Poland
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Poland
Ann Agric Environ Med. 2014;21(1):86–90
Introduction. Incomplete cross-linking of composite dental materials leads to their susceptibility to degradation in the environment of non-organic and organic solvents, contributing to the release of chemical compounds which are potentially harmful to living organisms. Objective. The aim of the study was an evaluation in in vitro conditions of releasing of potentially toxic substances from six dental composite materials available in EU countries. Materials and methods. The following compounds released from the samples stored in water were analyzed: bisphenol A (BPA), triethylene glycol-dimethacrylate (TEGDMA), urethane dimethacrylate (UDMA) and ethylene glycol dimethacrylate (EDGMA). Analysis of the substances was performed with the use of high performance liquid chromatography, after the following incubation periods: 1 hour, 24 hours, 7 days and 30 days. Results. Among the analyzed substances, after 1 hour of incubation, the highest average concentration was found for TEGDMA – 2045 μg cm-3 (in Herculite XRV material), after 24 hours – for UDMA 4.402 μg cm-3 (in Gradia Direct Anterior material) and after 7 and 30 days for TEGDMA: 8.112 and 6.458 μg•cm-3 respectively (in Charisma material). Conclusions. The examined composites used for reconstruction of hard tissues of teeth remain chemically unstable after polymerization, and release potentially harmful substances in conditions of the present study. The dynamics of the releasing of potentially harmful substances is correlated with the period of sample storage in water.
Rueggeberg FA. From vulcanite to vinyl, a history of resin in restorative dentistry. J Prosthet Dent. 2002; 87: 364–379.
Howard B, Wilson ND, Newman SM, Pfeifer CS, Stansbury JW. Relationship between conversion, temperature and optical properties during composite photopolymerization. Acta Biomater. 2010; 6: 2053–2059.
Leprince JG, Leveque P, Nystern B, Gallez B, Devaux J, Leloup G. New insight into the ”depht of cure” of dimethacrylane-based dental composites. Dent Mater. 2012; 28: 512–520.
Benetti AR, Asmussen E, Munksgaard EC. Softening and elution of monomers in ethanol. Dent Mater. 2009; 25: 1007–1013.
Bakopoulou A, Papadopoulos T, Gerefis P. Molecural toxicology of substances released from resin based dental restorative materials. Int J Molec Sci. 2009; 10: 3861–3899.
Polydorou O, Trittler R, Hellwig E, Kummerer K. Elution of monomers from two conventional dental composite materials. Dent Mater. 2007; 23: 1535–1541.
Szymanska J. Dental bioareosol as an occupational hazard in a dentist’s workplace. Ann Agric Environ Med. 2007; 14: 203–207.
Szymanska J: Exposure to airborne fungi during conservative dental treatment. Ann Agric Environ Med. 2006; 13: 177–179.
Moharamzadeh K, Brook IM, van Noort R. Biocompatibility of resin based dental materials. Dent Mater. 2009; 2: 514–548.
Eliades T, Voutsa D, Sifakakis I, Makou M, Katsaros CH. Release of bisphenol A from light-cured adhesive bonded to lingual fixed retainers. Am J Orthod Dent Orthop. 2011; 139: 192–195.
Chao H-H, Hang X-F, Chen B, Pan B, Hang L-J, Li L, Sun X-F, Shi Q-H, Shen W. Bisphenol A exposure modifies methylation of imprinted genes in Mouse oocytem via estrogen receptor signaling pathway. Histochem Cel Biol. 2012; 137: 249–259.
Hugo ER, Bradebourg TD, Woo JG, Loftus J, Alexander JW, BenJonathan N. Bisphenol A at environmentally relevant doses inhibits adiponectin release from human adipose tissue explants and adipocytes. Environ Health Perspect. 2008; 116(12): 1642–1647.
Midoro-Horiuti T, Tiwari R, Watson SCh, Goldblum RM. Maternal bisphenol A exposure promotes the development of experimental asthma in mouse pups. Environ Health Perspect. 2010; 118(2): 273–277.
Ishido M, Masuo Y, Terasaki M, Morita M. Rat hyperactivity by bisphenol A. but not by its derivatives, 3-hydroxybisphenol A or bisphenol A 3,4-quinone. Toxicol. Letters 2011; 206: 300–305.
Albericii-Martins C, Leyhausen G, Geurtsen W, Volk J. Intracellular glutatione: A main factor in TEGDMA-induced cytotoxicity? Dent Mater. 2012; 28: 442–448.
Eckhardt A, Harorli T, Limtanyakul J, Hiller KA, Bosl C, Bolay C, Reichl FX, Schmalz G, Schweikl H. Inhibition of cytokine and surface antigen expression in LPS-stimulated murine macrophages by triethylene glycol dimethacrylate. Biomater. 2009; 30: 1665–1674.
Urcan E, Scherthan H, Styllou M, Haertel U, Hickel R, Reichl FX. Induction od DNA double-strand breaks in primary gingival fibroblast by exposure to dental resin composites. Biomater. 2010; 31: 2010–2014.
Chang H-H., Chang M-Ch, Lin L-D, Lee J-J, Wang T-M, Huang ChH, Yang T-T, Lin H-J, Jeng J-H. The mechanism of cytotoxicity of urethane dimethacrylate to Chinese hamster ovary cells. Biomater. 2010; 31: 6917–6925.
Sidhu KK, Shaw S. Allergic contact dermatitis to acrylates in disposable blue diathermy pads. Ann R Coll Surg England. 1999; 81: 187–190.
Manojlovic D, Radisic M, Vasiljevic V, Zivkovic S, Lausevicb M, Miletic V. Monomer elution from nanohybrid and ormocer-based composites cured with different light sources. Dent Mater. 2011; 27: 371–378.
Bakapoulou A, Leyhausen G, Volk J, Tsiftsoglou A, Garefis P, Koidis P, Geurtsen W. Effects of HEMA and TEGDMA on the in vitro ordontogenic differentiation potential of human pulp stem/progenitor cells derived from decioduous teeth. Dent Mater. 2011; 27: 608–617.
Wisniewska-Jarosińska M, Poplawski T, Chojnacki CJ, Pawlowska E, Krupa R, Szczepanska R, Blłasiak J. Independent and combined cytotoxicity and genotoxicity of triethylene glycol dimethacrylate and urethane dimethacrylate. Mol Biol Rep. 2011; 38: 4603–4611.
Szczepanska J, Poplawski T, Synowiec E, Pawlowska E, Chojnacki CJ, Chojnacki J, Blasiak J. 2-Hydroxyetyl methacrylate (HEMA), a tooth restoration component, exerts its genotoxic effects in human gingival fibroblasts through methacrylic acid, an immediate product of its degradation. Mol Biol Rep. 2012; 39: 1561–1574.
European Commission Directive 2011/8/EU. 29.1.2011, L26, 11–14.
Update on bisphenol A for use in food contact applications U.S. Food and Drug Administration 2010 publichealthfocus/ucm 197739.htm.