Child car seats – a habitat for house dust mites and reservoir for harmful allergens
David Clarke 1, 2  
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School of Physics and Ryan Institute, National University of Ireland, Galway, Ireland
Applied Ecology Unit, School of Natural Sciences and Ryan Institute, National University of Ireland, Galway, Ireland
School of Mathematics, Statistics and Applied Mathematics, National University of Ireland, Galway, Ireland
Ann Agric Environ Med. 2015;22(1):17–22
Introduction and objective:
House dust mites produce allergens which can cause or aggravate diseases such as asthma, eczema and rhinitis. The objectives of this study are to quantify typical house dust mite and Der p 1 allergen levels in child car seats, and to determine external variables that may influence mite populations in cars.

Material and Methods:
Dust samples were collected from the child car seats and driver seats of 106 cars using a portable vacuum sampling pump over a two minute sampling period. Mites were counted and identified and results were expressed as mites per gram (mites/g) of dust, while Der p 1 content of samples were measured by enzyme-linked immunosorbent assay (ELISA). Questionnaires were completed by participants to identify environmental and behavioural effects on mite populations. Results were analysed using General Linear Model (GLM) procedures.

Twelve species of mites, of which nine are known to produce harmful allergens, were recorded from 212 dust samples. Over 80% of drivers’ seats and over 77% of child car seats harboured dust mites with a significant correlation (p = 0.001) between the mites/g of dust and Der p 1 content recovered from each seat. A mean of 53 mites/g of dust per seat was recovered, with a mean Der p 1 level of 1.1µg/g. Over 12% of driver seats and 15% of child car seats contained house dust mite levels sufficient to be risk factors for sensitisation and allergic reactions.

Child car seats and driver seats are habitats to a range of mite species which can be present in sufficient concentrations to cause or aggravate allergen related illnesses in individuals who are genetically predisposed.

Tovey ER, Willenborg CM, Crisafulli DA, Rimmer J, Marks GB. Most personal exposure to house dust mite aeroallergen occurs during the day. PLOS ONE. 2013; 8(7): e69900.
Hallas TE, Gislason T, Gislason D. Mite allergy and mite exposure in Iceland. Ann Agric Environ Med. 2011; 18(1): 13–17.
Asman M, Solarz K, Szilman E, Szilman P. Analysis of expression and amino acid sequence of the allergen Mag 3 in two species of house dust mites – Dermatophagoides farinae and D. pteronyssinus (Acari: Astigmata: Pyroglyphidae). Ann Agric Environ Med. 2010; 17(1): 45–48.
Henszel Ł, Kalisińska E, Kosik-bogacka DI, Kuźna-grygiel W. Mites in Dust Samples Collected from Sleeping Places in Apartments. Polish J of Environ Stud. 2010; 19(4): 723–730.
Arlian LG, Platts-Mills TAE. The biology of dust mites and the remediation of mite allergens in allergic disease. J Allergy Clin Immunol. 2001; 107(3 Suppl): S406–S413.
Solarz K. The allergenic acarofauna of house dust from dwellings, hospitals, libraries and institutes in upper Silesia (Poland). Ann Agric Environ Med. 1998; 5(1): 73–85.
Einarsson R, Munir AKM, Dreborg SKG. Allergens in school dust: II. Major mite (Der p I, Der f I) allergens in dust from Swedish schools. J Allergy Clin Immunol. 1995; 95(5 Pt 1): 1049–1053.
Wickens K, Martin I, Pearce N, Fitzharris P, Kent R, Holbrook N, et al. House dust mite allergen levels in public places in New Zealand. J Allergy Clin Immunol. 1997; 99(5): 587–593.
Uehara K, Toyoda Y, Konishi E. Contamination of passenger trains with Dermatophagoides (Acari: Pyroglyphidae) mite antigen in Japan. Exp Appl Acarol. 2000; 24(9): 727–734.
Partti-Pellinen K, Marttila O, Mäkinen-Kiljunen S, Haahtela T. Occurrence of dog, cat, and mite allergens in public transport vehicles. Allergy. 2000; 55(1): 65–68.
Ćustović A, Taggart S, Fletche A, Chapman M, Pickering C, Woodcock A. House dust mite allergens in public buildings and public transport. Proceedings: Mites, Asthma and Domestic Design II. University Printing Service, University of Sydney. 1995; 1: 89–91.
Taketomi EA, Justino CM, Pereira FL, Segundo GR, Sopelete MC, Sung SJ, et al. Taxis but not private cars are mite allergen reservoirs in Brazil. J Investig Allergol Clin Immunol. 2006; 16(1): 34–36.
Justino CM, Segundo GR, Pereira FL, Silva DA, Sopelete MC, Sung SS, et al. Mite and pet allergen exposure in Brazilian private cars. Ann Allergy Asthma Immunol. 1995; 94(6): 658–661.
Colloff MJ. Mite fauna of dust from passenger trains in Glasgow. Epidemiol Infect. 1987; 98(1): 127–130.
Neal JS, Arlian G, Morgan MS. Relationship amoung house-dust mites, Der 1, Fel d 1, and Can f 1 on clothing and automobile seats with respect to densities in houses. Ann Allergy Asthma Immunol. 2002; 88(4): 410–415.
Department of Transport National Travel Survey 2012.Material compiled and presented by the Department for Transport, Great Minster House, 33 Horseferry Road, London, SW1P 4DR.
Arbitron National In-Car Study: 2009 Edition. Arbitron Inc., 142 West 57th Street, New York, NY 10019: 212:887–1461.
Central Statistics Office Travel Report 2012. Material compiled and presented by the Central Statistics Office. Government Publications Sales Office, Sun Alliance House, Molesworth Street, Dublin 2.
Marks GB, Tovey ER, Toelle BG, Wachinger S, Peat JK, Woolcock AJ. Mite allergen (Der p 1) concentration in houses and its relation to the presence and severity of asthma in a population of Sydney schoolchildren. J Allergy Clin Immunol. 1995; 96(4): 441–448.
Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol. 1997; 100(6 Pt 1): S2-S24.
Cameron MM, Hill N. Permethrin-Impregnated Mattress Liners: a novel and effective intervention against house dust mites (Acari: Pyroglyphidae). J Med Entomol. 2002; 39(5): 755–762.
Baker AS. Mites and ticks of domestic animals: an identification guide and information source. London, The Natural History Museum, The Stationary Office, 1999.
Colloff MJ. Dust mites. Collingwood, Victoria, Australia, CSIRO, 2009.
Luczynska CM, Arruda LK, Platts-Mills TA, Miller JD, Lopez M, Chapman MD. A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p I and Der f I. J Immunol Methods. 1989; 118(2): 227–235.
Müsken H, Franz JT, Wahl R, Paap A, Cromwell O, Masuch G, et al. Sensitization to different mite species in German farmers: in vitro analyses. J Investig Allergol Clin Immunol. 2003; 13 (1): 26–35.
Xue-Ying Z, Zhen-Fu Z, Xin S, Zhi-Gang L, Yan-Si L. Localization of cross-reactivity allergens in the body of Tarsonemus granarius (Acari: Tarsonemidae) by immunohistochemistry. Acta Entomologica Sinica. 2011; 54(7): 848–852.
Terra SA, Silva DA, Sopelete MC, Mendes J, Sung SJ, Taketomi EA. Mite allergen levels and acarologic analysis in house dust samples in Uberaba, Brazil. J Investig Allergol Clin Immunol. 2004; 14(3): 232–237.
Mihrshahi S, Marks GB, Vanlaar C, Tovey E, Peat J. Predictors of high house dust mite allergen concentrations in residential homes in Sydney. Allergy. 2002; 57(2): 137–142.
Veale AJ, Peat JK, Tovey ER, Salome CM, Thompson JE, Woolcock AJ. Asthma and atopy in four rural Australian Aboriginal communities. Med J Aust. 1996; 165(4): 192–196.
Lah EF, Musa RN, Ming HT. Effect of germicidal UV-C light (254 nm) on eggs and adult of house dust mites, Dermatophagoides pteronyssinus and Dermatophagoides farinae (Astigmata: Pyroglyphidae). Asian Pac J Trop Biomed. 2012; 2(9): 679–83.
Krantz GW, Walter, DE. A Manual of Acarology. 3rd Edition. Lubbock, Texas, Texas Tech University Press, 2009.