Antimicrobial activity of two essential oils.
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Department of Food Safety and Animal Hygiene, Veterinary Academy of Lithuanian University of Health Science, Kaunas, Lithuania
Ruta Mickiene   

Department of Food Safety and Animal Hygiene, Veterinary Academy of Lithuanian University of Health Science, Tilzes str. 18, LT-47181 Kaunas, Lithuania
Ann Agric Environ Med. 2011;18(1):139–144
The aim of the study was to evaluate the antimicrobial activity of essential oils in vitro for possible application to reduce the content of microorganisms in the air of animal houses. The essential oils of Cymbopogon citrarus L. and Malaleuca alternifolia L. were screened against bacteria Staphylococcus aureus, Enterococcus faecium, Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis and yeast Candida albicans. The minimal inhibitory concentration of the active essential oils was tested using broth dilution assay. The essential oils concentrations ranged from 0.1-50.0 percent. The combined effects of essential oils were tested for Malaleuca alternifolia L. and Cymbopogon citrarus L. concentrations ranged from 0.005-50.0 percent. The oils showed a wide spectrum of antibacterial activity. Concentrations of 0.1-0.5 percent of Cymbopogon citrarus L. and Malaleuca alternifolia L. reduced total microorganisms count of Proteus mirabilis and Candida albicans. High antibacterial activity was also revealed for Cymbopogon citrarus L. with bactericidal concentrations of 0.8 percent for Escherichia coli, 5.0 percent for Enterococcus faecium, 5.0 percent for Pseudomonas aeruginosa and 8.0 percent for Staphylococcus aureus. Bactericidal concentrations of Malaleuca alternifolia L. were 5.0 percent for Pseudomonas aeruginosa and Enterococcus faecium, and 8.0 percent for Staphylococcus aureus. The essential oils of Cymbopogon citrarus and Malaleuca alternifolia may be a promising alternative of air disinfection in animal houses.
Andrews RE, Parks LW, Spence KD: Some effects of Douglas fi r terpenes on certain microorganisms. Appl Environ Microbiol 1980, 40, 301–304.
Bakutis B, Monstvilienė E, Januškevičienė G: Analyses of Airborne Contamination with Bacteria, Endotoxins and Dust in Livestock Barns and Poultry Houses. Acta Vet Brno 2004, 73, 283–289.
Burt S: Essential oils: their antibacterial properties and potential applications in foods – a review. Int J Food Microbiol 2004, 94, 223–253.
Carson CF, Mee BJ, Riley TV: Mechanism of action of Malaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by timekill, lysis, leakage, and salt tolerance assays and electron microscopy. Antimicrob Agents Chemother 2002, 46, 1914–1920.
Cox SD, Mann CM, Markham JL, Bell HC, Gustafson JE, Warmington JR, Wyllie SG: The mode of antimicrobial action of essential oil of Melaleuca alternifola (tea tree oil). J Appl Microbiol 2000, 88, 170–175.
Cox SD, Mann CM, Markham JLJ, Gustafson E, Warmington JR, Wyllie SG: Determining the antimicrobial actions of tea tree oil molecules. Molecules 2001, 6, 87–91.
Darokar MP, Mathur A, Dwivedi S, Bhalla R, Khanuja SPS, Kumar S: Detection of antibacterial activity in the fl oral petals of some higher plants. Curr Sci 1998, 75, 187–189.
Davidson PM, Parish ME: Methods for testing the effi cacy of food antimicrobials. Food Technol 1989, 43, 148–155.
Dorman HJD, Deans SG: Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 2000, 88, 308–316.
Dorman HJD: Phytochemistry and bioactive properties of plant volatile oils: antibacterial, antifungal and antioxidant activities. PhD Thesis. University of Strathclyde, Glasgow 1999.
Faid M, Anchad M, Bakhy K, Tantaoui-Elaraki A: Almond paste: Physico-chemical and microbiological characterization an preservation with sorbic acid and cinnamon. J Food Prot 1995, 58, 547–550.
Gill AO, Delaquis P, Russo P, Holley RA: Evaluation of antilisterial action of cilantro oil on vacuum packed ham. Int J Food Microbiol 2002, 73, 83-92.
Gustafson JE, Liew YC, Chew S, Markham JL, Bell HC, Wyllie SG, Warmington JR: Effects of tea tree oil on Escherichia coli. Lett Appl Microbiol 1998, 26, 194–198.
Hammer KA, Carson CF, Riley TV: Antifungal effects of Melaleuca alternifolia (tea tree) oil and its components on Candida albicans, Candida glabrata and Saccharomyces cerevisiae. J Antimicrob Chemother 2004, 53, 1081–1085.
Jackson RW, De Moss JA: Effects of toluene on Escherichia coli. J Bacteriol 1965, 90, 142.
Kordali S, Kotan R, Mavi A, Cakir A, Ala A, Yildirim A: Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum and Artemisia spicigera essential oils. J Agric Food Chem 2005, 53, 9452–9458.
Liebers V, Bruning T, Raulf-Heimsoth M: Occupational endotoxin-exposure and possible health effects on humans. Am J Ind Med 2006, 49, 474–491.
Lis-Balchin M, Deans SG, Eaglesham E: Relationship between bioactivity and chemical composition of commercial essential oils. Flavour Fragr J 1998, 13, 98–104.
Mackiewicz B: Study on exposure of pig farm workers to bioaerosols, immunologic reactivity and health effects. Ann Agric Environ Med 1998, 5, 169–175.
Marino M, Bersani C, Comi G: Impedance measurements to study the antimicrobial activity of essential oils from Lamiacea and Compositae. Int J Food Microbiol 2001, 67, 187–195.
Milhau G, Valentin A, Benoit F, Mallie M, Bastide J, Pelissier Y, Bessiere J: In vitro antimicrobial activity of eight essential oils. J Essent Oil Res 1997, 9, 329–333.
Milton DK, Walters MD, Hammond K, Evans JS: Worker exposure to endotoxin, phenolic compounds, and formaldehyde in a fi berglass insulation manufacturing plant. J Ind Hyg Assoc 1996, 57, 889–905.
Mourey A, Canillac N: Anti-Listeria monocytogenes activity of essential oils components of conifers. J Food Control 2002, 13, 289–292.
National Committee for Clinical Laboratory Standards: Performance standards for anti-microbial susceptibility testing: eleventh informational supplement. Document M 100-S11. NCCLS 2001 – Wayne, PA.
Nowak D: Health effects of airborne pollutants, particularly in swine confi nement stalls, from the viewpoint of occupational medicine. Dtsch Tierarztl Wochenschr 1998, 105, 225–234.
Radon K: The two sides of the “endotoxin coin”. Occup Environ Med 2006, 63, 73–78.
Rautiala S, Kangas J, Louhelainen K, Reiman M: Farmer’s exposure to airborne microorganisms in composting swine confi nement buildings. AIHA J (Fairfax, Va) 2003, 64, 673–677.
Rylander R: Endotoxin and occupational airway disease. Curr Opin Allergy Clin Immunol 2006, 6, 62–66.
Schwartz DA: TLR4 and LPS hyporesponsiveness in humans. Int J Hyg Environ Health 2002, 205(3), 221–227.
Sikkema J, De Bont JAM, Poolman B: Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 1995, 59, 201–222.
Sylvestre M, Pichette A, Longtin A, Nagau F, Legault J: Essential oil analysis and anticancer activity of leaf essential oil of Croton fl avens L. from Guadeloupe. J Ethnopharmacol 2006, 103, 99–102.
ibe S, Ramirez J, Peña A: Effects of beta-pinene on yeast membrane functions. J Bacteriol 1985, 161, 1195–1200.
Uribe S, Rangel P, Espínola G, Aguirre G: Effects of cyclohexane, an industrial solvent, on the yeast Saccharomyces cerevisiae and on isolated yeast mitochondria. Appl Environ Microbiol 1990, 56, 2114–2119.