A study of [i]Neospora caninum[/i] and [i]Toxoplasma[/i] [i]gondii[/i] antibody seroprevalence in healthy cattle in the Czech Republic

Eva Bártová 1,  
Kamil Sedlak 2,  
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
Department of Virology and Serology, State Veterinary Institute, Prague, Czech Republic
Department of Mathematics and Statistics, Faculty of Science, Masaryk University, Brno, Czech Republic
Ann Agric Environ Med 2015;22(1):32–34
[b]Introduction and objective[/b]. The aim of study was to test the sera of healthy dairy cows by ELISAs, the methods also used in other groups of animals in the Czech Republic, and thus to obtain actual data about [i]N. caninum[/i] and [i]T. gondii[/i] seroprevalence in cattle. [b]Material and methods[/b]. In the Czech Republic, sera from 546 clinically healthy dairy cows ([i]Bos primigenius f. taurus[/i]) aged > 2 years from 49 farms in 7 districts were collected. Sera were tested for [i]Neospora caninum[/i] antibodies by a commercial competitive-inhibition enzyme-linked immunosorbent assay; samples with more than 30% inhibition were considered as positive. The same samples were also analysed for Toxoplasma-specific IgG antibodies by an enzyme-linked immunosorbent assay; samples with more than 50% S/P were considered as positive. [b]Results[/b]. Antibodies against [i]N. caninum[/i] were found only in 3 cows (0.5%) with inhibitions of 47, 78 and 85. Antibodies against [i]T. gondii [/i]were found in 53 cows (9.7%) with S/P ranging from 51% to over 211%; positive animals were found in 4 of 7 districts, with prevalences ranging from 8% – 14%. Indication of mixed infections (concurrent presence of both [i]N. caninum[/i] and [i]T. gondii [/i]antibodies) was not proved. [b]Conclusion[/b]. The results of the study indicate that dairy cows in the Czech Republic have a relatively low seroprevalence for both [i]N. caninum[/i] and [i]T. gondii[/i]. Therefore, natural infection with [i]N. caninum[/i] and [i]T. gondii[/i] seems not to be very common in Czech cattle. These results show actual data about [i]N. caninum[/i] and [i]T. gondii [/i]infection in healthy dairy cattle from the Czech Republic.