RESEARCH PAPER
The effect of MLS laser radiation on cell lipid membrane
| 1 | Department of Medical Rehabilitation, Faculty of Military Medicine, Medical University of Lodz, Poland |
| 2 | Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Poland |
CORRESPONDING AUTHOR
Kamila Pasternak
Department of Medical Rehabilitation, Faculty of Military Medicine, Medical University of Lodz, Poland
Department of Medical Rehabilitation, Faculty of Military Medicine, Medical University of Lodz, Poland
Ann Agric Environ Med. 2018;25(1):108–113
KEYWORDS
ABSTRACT
Introduction:
Authors of numerous publications have proved the therapeutic effect of laser irradiation on biological material, but the mechanisms at cellular and subcellular level are not yet well understood.
Objective:
The aim of this study was to assess the effect of laser radiation emitted by the MLS M1 system (Multiwave Locked System) at two wavelengths (808 nm continuous and 905 nm pulsed) on the stability and fluidity of liposomes with a lipid composition similar to that of human erythrocyte membrane or made of phosphatidylocholine.
Material and Methods:
Liposomes were exposed to low-energy laser radiation at surface densities 195 mW/cm2 (frequency 1,000 Hz) and 230 mW/cm2 (frequency 2,000 Hz). Different doses of radiation energy in the range 0–15 J were applied. The surface energy density was within the range 0.46 – 4.9 J/cm 2.
Results:
The fluidity and stability of liposomes subjected to such irradiation changed depending on the parameters of radiation used.
Conclusions:
Since MLS M1 laser radiation, depending on the parameters used, affects fluidity and stability of liposomes with the lipid content similar to erythrocyte membrane, it may also cause structural and functional changes in cell membranes.
Authors of numerous publications have proved the therapeutic effect of laser irradiation on biological material, but the mechanisms at cellular and subcellular level are not yet well understood.
Objective:
The aim of this study was to assess the effect of laser radiation emitted by the MLS M1 system (Multiwave Locked System) at two wavelengths (808 nm continuous and 905 nm pulsed) on the stability and fluidity of liposomes with a lipid composition similar to that of human erythrocyte membrane or made of phosphatidylocholine.
Material and Methods:
Liposomes were exposed to low-energy laser radiation at surface densities 195 mW/cm2 (frequency 1,000 Hz) and 230 mW/cm2 (frequency 2,000 Hz). Different doses of radiation energy in the range 0–15 J were applied. The surface energy density was within the range 0.46 – 4.9 J/cm 2.
Results:
The fluidity and stability of liposomes subjected to such irradiation changed depending on the parameters of radiation used.
Conclusions:
Since MLS M1 laser radiation, depending on the parameters used, affects fluidity and stability of liposomes with the lipid content similar to erythrocyte membrane, it may also cause structural and functional changes in cell membranes.
REFERENCES (23)
1.
Chow RT, Johnson MI, Lopes-Martins RA, Bjordal JM. Efficacy of low-level laser therapy in the management of neck pain: a systematic review and meta-analysis of randomised placebo or active-treatment controlled trials. The Lancet. 2009; 374(9705): 1897 – 908.
2.
Hegedüs B, Viharos L, Gervain M, Gálfi M. The Effect of Low-Level Laser in Knee Osteoarthritis: A Double-Blind, Randomized, Placebo-Controlled Trial. Photomed Laser Surg. 2009; 27(4): 577–84.
3.
Hashmi JT, Huang YY, Osmani BZ, Sharma SK, Naeser MA, Hamblin MR. Role of low-level laser therapy in neurorehabilitation. PM&R. 2010; 2(12 Suppl 2): 292–305.
4.
Ivandic BT, Ivandic T. Low-Level Laser Therapy Improves Visual Acuity in Adolescent and Adult Patients with Amblyopia. Photomed Laser Surg. 2012; 11: 167–71.
5.
Mahram M, Rajabi M. Treatment of Lymphedema Praecox through Low Level Laser Therapy (LLLT). J Res Med Sci. 2011; 16(6): 848–51.
6.
Mesquita-Ferrari RA, Ribeiro R, Souza NH, Silva CA, Martins MD, Bussadori SK, et al. No effect of low-level lasers on in vitro myoblast culture. Indian J Exp Biol. 2011; 49(6): 423–8.
7.
Wu Q, Xuan W, Ando T, Xu T, Huang L, Huang YY, Dai T, Dhital S, et al. Low-level laser therapy for closed-head traumatic brain injury in mice: effect of different wavelengths. Lasers Surg Med. 2012; 24: 218–26.
8.
Pagnutti S. Scientific Report MLS Therapy. Cutting Edge Laser Technology Press. Italy, 2004; 5–23.
9.
Mognato M, Squizzato F, Facchin F, Zaghetto L, Corti L. Cell growth modulation of human cells irradiated in vitro with low-level laser therapy. Photomed Laser Surg. 2004; 22(6): 523–6.
10.
Huang YY, Chen AC, Carroll JD, Hamblin MR. Biphasic dose response in low level light therapy. Dose Response. 2009; 7(4): 358–83.
11.
Tafur J, Mills PJ. Low-intensity light therapy: exploring the role of redox mechanisms. Photomed Laser Surg. 2008; 26: 323–8.
12.
Gao X, Xing D. Molecular mechanisms of cell proliferation induced by low power laser irradiation. J Biomed Sci. 2009; 16: 4–14.
13.
Freitinger–Skalicka Z, Navratil L, Zolzer F, Hon Z. The Assessments of the Intracellular Antioxidant Protection of the Organism after LLLT Irradiation. AIP Conference Proceedings 2009; 1142(1): 87–91.
14.
Dremza IK, Lapshina EA, Kujawa J, Zavodnik IB. Oxygen-related processes in red blood cells exposed to tert-butyl hydroperoxide. Redox Report. 2006; 11(4): 185–92.
15.
Albertini R, Villaverde AB, Aimbire F, Salgado MAC, Bjordal JM, Alves LP, et al. Anti-inflammatory effects of low-level laser therapy (LLLT) with two different red wavelengths (660 nm and 684 nm) in carrageenan-induced rat paw edema. J Photochem Photobiol B. 2007; 89: 50–5.
16.
Gavish L, Perez L, Gertz SD. Low-level laser irradiation modulates matrix metalloproteinase activity and gene expression in porcine aortic smooth muscle cells. Lasers Surg Med. 2006; 38: 779–86.
17.
Chen ACH, Arany PR, Huang YY, Tomkinson EM, Saleem T, Yull FE, Blackwell TS, Hamblin MR. Low level laser therapy activates NF-κB via generation of reactive oxygen species in mouse embryonic fibroblasts. PLoS One. 2011; 6(7): e22453.
18.
Kujawa J, Zavodnik L, Zavodnik I, Buko V, Lapshyna A, Bryszewska M. Effect of low-intensity (3.75–25 J/cm2) near-infrared (810 nm) laser radiation on red blood cell ATPase activities and membrane structure. J Clin Laser Med Surg. 2004; 22(2): 111–7.
19.
Kujawa J. Molekularne i błonowe mechanizmy biostymulacyjnych efektów promieniowania laserowego o długości fali = 810nm.(Molecular and membrane mechanisms of biostimulating effects of laser radiation with wavelength of 810 nm) Habilitation dissertation in medical science in the field of medicine. Łodz. Medical University of Lodz Press, 2004.
20.
Piasecka A, Leyko W, Krajewska E, Bryszewska M. Effect of combined treatment with perindoprilat and low-power red light laser irradiation on human erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity. Scand J Clin Lab Invest. 2000; 60(5): 395–402.
21.
Kujawa J, Zavodnik L, Zavodnik I, Bryszewska M. Low-intensity near-infrared laser radiation-induced changes of acetylcholinesterase activity of human erythrocytes. J Clin Laser Med Surg. 2003; 21(6): 351–5.
22.
Wu JY, Chen CH, Wang CZ, Ho ML, Yeh ML, Wang YH. Low-power laser irradiation suppresses inflammatory response of human adipose-derived stem cells by modulating intracellular cyclic AMP level and NF-κB activity. PLoS One. 2013; 8(1): e54067.
23.
Giannelli M, Chellini F, Sassoli C, Francini F, Pini A, Squecco R, Nosi D, Bani D, Zecchi-Orlandini S, Formigli L.Source. Photoactivation of bone marrow mesenchymal stromal cells with diode laser: effects and mechanisms of action. J Cell Physiol. 2013; 228(1): 172–81.
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
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