Introduction: Authors of numerous publications have proved the therapeutic effect of laser irradiation on biological material, but the mechanisms at cellular and subcellular level are not yet well understood.
Objective: The aim of this study was to assess the effect of laser radiation emitted by the MLS M1 system (Multiwave Locked System) at two wavelengths (808 nm continuous and 905 nm pulsed) on the stability and fluidity of liposomes with a lipid composition similar to that of human erythrocyte membrane or made of phosphatidylocholine.
Material and Methods: Liposomes were exposed to low-energy laser radiation at surface densities 195 mW/cm2 (frequency 1,000 Hz) and 230 mW/cm2 (frequency 2,000 Hz). Different doses of radiation energy in the range 0–15 J were applied. The surface energy density was within the range 0.46 – 4.9 J/cm 2.
Results: The fluidity and stability of liposomes subjected to such irradiation changed depending on the parameters of radiation used.
Conclusions: Since MLS M1 laser radiation, depending on the parameters used, affects fluidity and stability of liposomes with the lipid content similar to erythrocyte membrane, it may also cause structural and functional changes in cell membranes.
REFERENCES(23)
1.
Chow RT, Johnson MI, Lopes-Martins RA, Bjordal JM. Efficacy of low-level laser therapy in the management of neck pain: a systematic review and meta-analysis of randomised placebo or active-treatment controlled trials. The Lancet. 2009; 374(9705): 1897 – 908.
Hegedüs B, Viharos L, Gervain M, Gálfi M. The Effect of Low-Level Laser in Knee Osteoarthritis: A Double-Blind, Randomized, Placebo-Controlled Trial. Photomed Laser Surg. 2009; 27(4): 577–84.
Mesquita-Ferrari RA, Ribeiro R, Souza NH, Silva CA, Martins MD, Bussadori SK, et al. No effect of low-level lasers on in vitro myoblast culture. Indian J Exp Biol. 2011; 49(6): 423–8.
Wu Q, Xuan W, Ando T, Xu T, Huang L, Huang YY, Dai T, Dhital S, et al. Low-level laser therapy for closed-head traumatic brain injury in mice: effect of different wavelengths. Lasers Surg Med. 2012; 24: 218–26.
Mognato M, Squizzato F, Facchin F, Zaghetto L, Corti L. Cell growth modulation of human cells irradiated in vitro with low-level laser therapy. Photomed Laser Surg. 2004; 22(6): 523–6.
Freitinger–Skalicka Z, Navratil L, Zolzer F, Hon Z. The Assessments of the Intracellular Antioxidant Protection of the Organism after LLLT Irradiation. AIP Conference Proceedings 2009; 1142(1): 87–91.
Albertini R, Villaverde AB, Aimbire F, Salgado MAC, Bjordal JM, Alves LP, et al. Anti-inflammatory effects of low-level laser therapy (LLLT) with two different red wavelengths (660 nm and 684 nm) in carrageenan-induced rat paw edema. J Photochem Photobiol B. 2007; 89: 50–5.
Kujawa J, Zavodnik L, Zavodnik I, Buko V, Lapshyna A, Bryszewska M. Effect of low-intensity (3.75–25 J/cm2) near-infrared (810 nm) laser radiation on red blood cell ATPase activities and membrane structure. J Clin Laser Med Surg. 2004; 22(2): 111–7.
Kujawa J. Molekularne i błonowe mechanizmy biostymulacyjnych efektów promieniowania laserowego o długości fali = 810nm.(Molecular and membrane mechanisms of biostimulating effects of laser radiation with wavelength of 810 nm) Habilitation dissertation in medical science in the field of medicine. Łodz. Medical University of Lodz Press, 2004.
Piasecka A, Leyko W, Krajewska E, Bryszewska M. Effect of combined treatment with perindoprilat and low-power red light laser irradiation on human erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity. Scand J Clin Lab Invest. 2000; 60(5): 395–402.
Kujawa J, Zavodnik L, Zavodnik I, Bryszewska M. Low-intensity near-infrared laser radiation-induced changes of acetylcholinesterase activity of human erythrocytes. J Clin Laser Med Surg. 2003; 21(6): 351–5.
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