RESEARCH PAPER
Prevalence and distribution of VRE (vancomycin resistant enterococci) and VSE (vancomycin susceptible enterococci) strains in the breeding environment
1
Department of Microbiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum of L. Rydygier in Bydgoszcz, Poland
2
Department of Microbiology and Food Technology, University of Technology and Life Sciences, Bydgoszcz, Poland
Corresponding author
Krzysztof Skowron
Department of Microbiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum of L. Rydygier in Bydgoszcz, Poland
Department of Microbiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum of L. Rydygier in Bydgoszcz, Poland
Ann Agric Environ Med. 2016;23(2):231-236
KEYWORDS
ABSTRACT
Introduction:
Intensive animal production causes numerous problems. Facilities connected with animal maintenance not only cause environmental pollution, but also pose a great sanitary and epidemiological threat. Long-term use of antibiotics in animal production lead animal-borne microorganisms to develop multiple resistance mechanisms, transferred to the typical environmental bacteria.
Objective:
The aim of this study was assessment of E. faecalis, E. faecium, E. durans and E. hirae prevalence in samples gathered from swine production sectors, and determination of the contribution of VRE (vancomycin resistant enterococci) strains and their resistance. The degree of relationship between isolates of each species from genus Enterococcus was also determined.
Material and Methods:
195 isolates were obtained, from which DNA was isolated. Genus identification was conducted with the primers specific to the 16S rRNA region, and identification of the species with primers specific to sequence of gene sodA in Multiplex PCR reaction. Resistance to vancomycin (6 μg×ml -1) was tested using a screening method on Muller Hinton Agar. To assess resistance type Multiplex PCR, amplifying products corresponding to genes VanA, VanB and VanC, was conducted. Genotyping was conducted using the PCR-RAPD method.
Results:
Among the 195 isolates, 133 (68%) belonged to E. hirae. The other species contributions were respectively: E. faecalis – 21%, E. durans – 8% and E. faecium – 3%. Only 2 isolates of E. hirae, being different strains, were resistant to vancomycin. Both were representing phenotype VanC1. 60 genetically different strains were defined. The possible contamination paths involved animal feed and spreading of excrements by slaughtered individuals or on personnel’s footwear.
Conclusions:
The obtained results indicate a very low percentage of VRE strains in the tested piggery, resulting in a low health risk to piggery, slaughterhouse or abattoir employees.
Intensive animal production causes numerous problems. Facilities connected with animal maintenance not only cause environmental pollution, but also pose a great sanitary and epidemiological threat. Long-term use of antibiotics in animal production lead animal-borne microorganisms to develop multiple resistance mechanisms, transferred to the typical environmental bacteria.
Objective:
The aim of this study was assessment of E. faecalis, E. faecium, E. durans and E. hirae prevalence in samples gathered from swine production sectors, and determination of the contribution of VRE (vancomycin resistant enterococci) strains and their resistance. The degree of relationship between isolates of each species from genus Enterococcus was also determined.
Material and Methods:
195 isolates were obtained, from which DNA was isolated. Genus identification was conducted with the primers specific to the 16S rRNA region, and identification of the species with primers specific to sequence of gene sodA in Multiplex PCR reaction. Resistance to vancomycin (6 μg×ml -1) was tested using a screening method on Muller Hinton Agar. To assess resistance type Multiplex PCR, amplifying products corresponding to genes VanA, VanB and VanC, was conducted. Genotyping was conducted using the PCR-RAPD method.
Results:
Among the 195 isolates, 133 (68%) belonged to E. hirae. The other species contributions were respectively: E. faecalis – 21%, E. durans – 8% and E. faecium – 3%. Only 2 isolates of E. hirae, being different strains, were resistant to vancomycin. Both were representing phenotype VanC1. 60 genetically different strains were defined. The possible contamination paths involved animal feed and spreading of excrements by slaughtered individuals or on personnel’s footwear.
Conclusions:
The obtained results indicate a very low percentage of VRE strains in the tested piggery, resulting in a low health risk to piggery, slaughterhouse or abattoir employees.
REFERENCES (24)
1.
Cormier Y, Boulet LP, Bedard G, Tremblay G. Respiratory health of workers exposed to swine confinement buildings only or to both swine confinement buildings and dairy barns. Scand. J. Work Env Hea. 1991; 17(4): 269–75.
2.
Kołacz R, Dobrzański Z. Higiena i dobrostan zwierząt gospodarskich. Wydawnictwo Akademii Rolniczej we Wrocławiu, 2006.
3.
Gilmore MS. The Enterococci: Pathogenesis, Molecular Biology, and Antibiotic Resistance. ASM Press, 2002.
4.
Chang CW, Chung H, Huang CF, Su HJJ. Exposure of Workers to Airborne Microorganisms in Open-Air Swine Houses. Farm animals as a putative reservoir for vancomycin-resistant enterococcal infection in man. J Antimicrob Chemoth. 1994; 34(4): 507–514.
5.
Leclercq R, Courvalin P. Resistance to glycopeptides in enterococci. Clin Infect Dis. 1997; 24: 545–554.
6.
Salem-Bekhit MM, Moussa IM, Muharram MM, Alanazy FK, Hefni HM. Prevalence and antimicrobial resistance pattern of multidrug-resistant enterococci isolated from clinical specimens. Indian J Med Microbiol. 2012; 30(1): 44–51.
7.
Shepard BD, Gilmore MS. Antibiotic-resistant enterococci: the mechanisms and dynamics of drug introduction and resistance. Microbes Infect. 2002; 4: 215–240.
8.
Bates J, Jordens JZ, Griffiths DT. Farm animals as a putative reservoir for vancomycin-resistant enterococcal infection in man. J Antimicrob Chemoth. 1994; 34(4): 507–514.
9.
Pérez-Rodríguez MT, Sopeña B, Longueira R, Lamas JL, Martínez-Vázquez C. Calf pyomyositis caused by Enterococcus faecalis, Calf pyomyositis caused by Enterococcus faecalis. Int J Med. 2010; 104 (6): 527.
11.
Willey BM, Kreiswirth BN, Simor AE, Willaims G, Scriver SR, Phillips A, et al. Detection of Vancomycin Resistance in Enterococcus Species. J Clin Microbiol. 1992; 30 (7): 1621–1624.
12.
Jackson CR, Fedorka-Cray PJ, Barrett JB. Use of a Genus- and Species-Specific Multiplex PCR for Identification of Enterococci. J Clin Microbiol. 2004; 42 (8): 3558–3565.
13.
Hans-Jurg M, Quednau M, Samuelsson A, Isaksson B, Ahrne S, Jonasson J. Division of the genus Enterococcus into species groups using PCR-based molecular typing methods. Microbiology. 1998; 144(5): 1171–1179.
14.
Perez-Hernandez X, Mendez-Alvarez S, Claverie-Martna F. A PCR assay for rapid detection of vancomycin-resistant enterococci. Diagn Micr Infec Dis. 2002; 42: 273–277.
15.
Kluczek JP. Charakterystyka bakteriologiczna warchlakarni. Pr Kom Nauk Roln i Biol. 1998; 44: 63–68.
16.
Kluczek Sz. Ogólna ocena flory bakteryjnej przemysłowej fermy trzody chlewnej. Pr Kom Nauk Roln i Biol. 1999; 46: 50–59.
17.
Kühn I. Epidemiology and ecology of enterococci, with special reference to antibiotic resistant strains, in animals, humans and the environment. Int J Antimicrob Ag. 2000; 14: 337–342.
18.
Garcia-Migura L, Pleydell E, Barnes S, Davies RH, Liebana E. Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Broiler Poultry and Pig Farms in England and Wales. J Clin Microbiol. 2005; 43 (7): 3283–9.
19.
Hershberger E. Epidemiology of antimicrobial resistance in enterococci of animal origin. J Antimicrob Chemoth. 2005: 127–130.
20.
Huycke MM, Sahm DF, Gilmore MS. Multiple-drug resistant enterococci: the nature of the problem and an agenda for the future. Emerg Infect Dis. 1998; 4(2): 239–249.
21.
Dever LL, China C, Eng RHK, O’Donovan C, Johanson WG. Vancomycin-resistant Enterococcus faecium in a Veterans Affairs Medical Center: Association with antibiotic usage. 34th Interscience Conference on Antimicrobial Agents and Chemotherapy; Oct 4 – 7 1994; Orlando, Florida; Washington, American Society for Microbiology, 1995.
22.
Ruzauskas M, Virgailis M, Šiugždinienė R, Sužiedėlienė E, Šeputienė V, Daugelavičius R, et al. Antimicrobial resistance of Enterococcus spp. isolated from livestock in Lithuania. Veterinarski Archiv. 2009; 79 (5): 439–449.
23.
Molla B, Stermann A, Mathews J, Artuso-Ponte V, Abley M, Farmer W, et al. Salmonella enterica in Commercial Swine Feed and Subsequent Isolation of Phenotypically and Genotypically Related Strains from Fecal Samples. Appl Environ Microbiol. 2010; 76 (21): 7188–7193.
24.
Purwin C, Lipiński K, Pysera B. Jakość higieniczna kiszonek, Życie Weterynaryjne. Życie Wet. 2012; 87(1): 37–40.
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