RESEARCH PAPER
First report of Bartonella sp. isolated from Hippobosca equina L. (Hippoboscidae: Hippobosca) in Lublin Province, south-eastern Poland
1
Department of Animal Ethology and Wildlife Management, University of Life Sciences, Lublin, Poland
2
Faculty of Medical Sciences in Zabrze, Department of Medical and Molecular Biology, Medical University of Silesia, Katowice, Poland
3
Department of Biology and Parasitology, Faculty of Health Sciences, Medical University, Lublin, Poland
4
Department of Zoology and Genetics, Faculty of Biology, University of Bialystok, Poland
Corresponding author
Weronika Maślanko
Department of Animal Ethology and Wildlife Management, University of Life Sciences in Lublin, Akademicka 13 St.,, 20-950, Lublin, Poland
Department of Animal Ethology and Wildlife Management, University of Life Sciences in Lublin, Akademicka 13 St.,, 20-950, Lublin, Poland
KEYWORDS
TOPICS
ABSTRACT
Introduction and objective:
Hippobosca equina (Diptera: Hippoboscidae), is a widespread blood-feeding ectoparasite associated with the forest ecosystem. The insect is characterized by a wide host range and low host specificity, which increases the risk of feeding on animals that constitute a reservoir of transmissible pathogens, including Bartonella spp.
Material and methods:
Hippobosca equina adults were collected from humans and companion animals within a continental mesotrophic oak-pine mixed forest in eastern Poland. DNA was isolated by the ammonia method, and isolates obtained from single individuals were tested by PCR method for the presence of 5 vector-borne pathogens. In case of the positive results, the amplicons were sequenced and examined by a BLAST search.
Results:
The PCR analysis of DNA isolates obtained from 100 H. equina specimens revealed the presence of the RNA polymerase beta-subunit gene (rpoB) of the genus Bartonella, in 1% of the studied insects, i.e. one H. equina female. The rpoB gene haplotype of Bartonella sp. reported in this study, was identical to a Bartonella sp. sequence obtained from deer keds in Lithuania, and very closely related to strains with zoonotic potential. None of the H. equina specimens studied was positive for the presence of B. burgdorferi s.l., Anaplasma phagocytophilum, Babesia spp., and Coxiella burnetii.
Conclusions:
The study indicates the need to screen the occurrence of Bartonella spp., both in potential vectors and reservoirs of this pathogen in various habitats.
Hippobosca equina (Diptera: Hippoboscidae), is a widespread blood-feeding ectoparasite associated with the forest ecosystem. The insect is characterized by a wide host range and low host specificity, which increases the risk of feeding on animals that constitute a reservoir of transmissible pathogens, including Bartonella spp.
Material and methods:
Hippobosca equina adults were collected from humans and companion animals within a continental mesotrophic oak-pine mixed forest in eastern Poland. DNA was isolated by the ammonia method, and isolates obtained from single individuals were tested by PCR method for the presence of 5 vector-borne pathogens. In case of the positive results, the amplicons were sequenced and examined by a BLAST search.
Results:
The PCR analysis of DNA isolates obtained from 100 H. equina specimens revealed the presence of the RNA polymerase beta-subunit gene (rpoB) of the genus Bartonella, in 1% of the studied insects, i.e. one H. equina female. The rpoB gene haplotype of Bartonella sp. reported in this study, was identical to a Bartonella sp. sequence obtained from deer keds in Lithuania, and very closely related to strains with zoonotic potential. None of the H. equina specimens studied was positive for the presence of B. burgdorferi s.l., Anaplasma phagocytophilum, Babesia spp., and Coxiella burnetii.
Conclusions:
The study indicates the need to screen the occurrence of Bartonella spp., both in potential vectors and reservoirs of this pathogen in various habitats.
ACKNOWLEDGEMENTS
The authors express their thanks to the National Veterinary
Institute-National Research Institute in Puławy, eastern
Poland, for providing the DNA isolate of Coxiella burnetii,
and to Ewelina Szwaj PhD for assistance in the identification
of specimens, and to Joanna Witecka PhD for assistance in
the molecular analyses.
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