First detection and molecular characterization of hepatitis E virus in water from wastewater treatment plants in Portugal
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Centro de Investigação em Engenharia dos Processos Químicos e dos Produtos da Floresta (CIEPQF), Faculdade de Farmácia da Universidade de Coimbra, Portugal
Agrarian Superior School, Polytechnic Institute of Viseu, Portugal
Laboratório de Virologia, Grupo das Biociências Clínicas e Aplicadas, Faculdade de Farmácia da Universidade de Coimbra, Portugal
Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal
Corresponding author
João R Mesquita   

Agrarian Superior School, Polytechnic Institute of Viseu, Quinta da Alagoa, Estrada de Nelas, 3500-606 Viseu, Portugal
Ann Agric Environ Med. 2018;25(2):364-367
Hepatitis E virus (HEV) genotype 3 is widespread in industrialized countries and widely recognized as a serious public health issue. HEV genotype 3 is excreted in both human and animal stools, having the potential to be introduced in aquatic environments through wastewater discharge. For this reason, waterborne transmission of the HEV genotype 3 is drawing increasing attention from the scientific community. Although studies in Portugal have shown that HEV circulates in both humans and swine, no study has yet focused on the presence of HEV in wastewater. Hence, a total of 60 water samples (influent and effluent) from 15 Wastewater Treatment Plants (WWTP) of different regions of Portugal were tested for the presence of HEV using a broad spectrum real-time RT-PCR with amplification within the ORF2 region of HEV genome. Positive samples were retested by nested RT-PCR with amplification within the ORF1 region, sequenced followed by phylogenetic analysis. HEV sequences were retrieved from two influent samples collected in December 2013 from WWTPs of North and Central of Portugal and classified in subgenotype 3i and 3f. This is the first study showing the presence of HEV in aquatic environments of Portugal.

Smith DB, Simmonds P, & International Committee on Taxonomy of Viruses Hepeviridae Study Group. Consensus proposals for classification of the family Hepeviridae. J Gen Virol. 2014; 95: 2223-2232.
Berto A, Backer JA, Mesquita JR, Nascimento MS, Banks M, Martelli F, Ostanello F, Angeloni G, Di Bartolo I, Ruggeri FM, Vasickova P, Diez-Valcarce M, Hernandez M, Rodriguez-Lazaro D, van der Poel WH. Prevalence and transmission of hepatitis E virus in domestic swine populations in different European countries. BMC Res Notes. 2012; 25(5): 190.
Teixeira J, Mesquita JR, Pereira SS, Oliveira RM, Abreu-Silva J, Rodrigues A, Myrmel M, Stene-Johansen K, Øverbø J, Gonçalves G, Nascimento MS. Prevalence of hepatitis E virus antibodies in workers occupationally exposed to swine in Portugal. Med Microbiol Immunol. 2017; 206(1): 77-81.
Van der Poel WH. Food and environmental routes of Hepatitis E virus transmission. Curr Opin Virol. 2014; 4: 91-96.
Ouardani I, Manso CF, Aoun M, Romalde JL. Efficiency of hepatitis A virus removal in six sewage treatment plants from central Tunisia. Appl Microbiol Biotechnol. 2015; 99: 10759-69.
Prevost B, Lucas FS, Goncalves A, Richard F, Moulin L, Wurtzer S. Large scale survey of enteric viruses in river and waste water underlines the health status of the local population. Environ Int. 2015; 79: 42-50.
Mesquita JR, Oliveira D, Rivadulla E, Abreu-Silva J, Varela MF, Romalde JL, Nascimento MS. Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain. Food Microbiol. 2016; 58: 13-15.
Martínez Wassaf MG, Pisano MB, Barril PA, Elbarcha OC, Pinto MA, Mendes de Oliveira J, DiGiusto P, Nates SV, Ré VE. First detection of hepatitis E virus in Central Argentina: environmental and serological survey. J Clin Virol. 2014; 61: 334-9.
Li H, Li W, She R, Yu L, Wu Q, Yang J, Hu F, Soomro MH, Shi R, Hao W, Zhao Y, Mao J. Hepatitis E Virus Genotype 4 Sequences Detected in Sewage from Treatment Plants of China. Food Environ Virol. 2017; 9: 230-233.
Masclaux FG, Hotz P, Friedli D, Savova-Bianchi D, Oppliger A. High occurrence of hepatitis E virus in samples from wastewater treatment plants in Switzerland and comparison with other enteric viruses. Water Res. 2013; 47: 5101-9.
Oliveira R, Mesquita JR, Pereira S, Abreu-Silva J, Teixeira J, Nascimento MSJ. Seroprevalence of Hepatitis E Virus Antibodies in Portuguese Children. Pediatr Infect Dis J. 2017; 36: 623-626.
Nascimento MSJ, Pereira SS, Teixeira J, Abreu-Silva J, Oliveira RMS, Myrmel M, Stene-Johansen K, Øverbø J, Gonçalves G, Mesquita JR. A nationwide serosurvey of hepatitis E virus antibodies in the general population of Portugal. Eur J Public Health. 2017. 11. [Epub ahead of print].
Mesquita JR, Oliveira RM, Coelho C, Vieira-Pinto M, Nascimento MS. Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal. Transbound Emerg Dis. 2016; 63: 574-8.
Rafique A, Jiang S. Genetic diversity of human polyomavirus JCPyV in Southern California wastewater. J Water Health. 2008; 6: 533–538.
Rolfe KJ, Curran MD, Mangrolia N, Gelson W, Alexander GJ, L'estrange M, Vivek R, Tedder R, Ijaz S. First case of genotype 4 human hepatitis E virus infection acquired in India. J Clin Virol. 2010 May; 48(1): 58-61.
Johne R, Plenge-Bönig A, Hess M, Ulrich RG, Reetz J, Schielke A. Detection of a novel hepatitis E-like virus in faeces of wild rats using a nested broad-spectrum RT-PCR. J Gen Virol. 2010 Mar; 91(Pt 3): 750-8.
Santos L, Mesquita JR, Rocha Pereira N, Lima-Alves C, Serrão R, Figueiredo P, Reis J, Simões J, Nascimento M, Sarmento A. Acute hepatitis E complicated by Guillain-Barre syndrome in Portugal, December 2012--a case report. Euro Surveill. 2013 Aug 22; 18(34). pii: 20563.
Barril PA, Giordano MO, Isa MB, Masachessi G, Ferreyra LJ, Castello AA, Glikmann G, Nates SV. Correlation between rotavirus A genotypes detected in hospitalized children and sewage samples in 2006, Córdoba, Argentina. J Med Virol. 2010 Jul; 82(7): 1277-81.
Prado T, Fumian TM, Miagostovich MP, Gaspar AM. Monitoring the hepatitis A virus in urban wastewater from Rio de Janeiro, Brazil. Trans R Soc Trop Med Hyg. 2012 Feb; 106(2): 104-9.
Hellmér M, Paxéus N, Magnius L, Enache L, Arnholm B, Johansson A, Bergström T, Norder H. Detection of pathogenic viruses in sewage provided early warnings of hepatitis A virus and norovirus outbreaks. Appl Environ Microbiol. 2014 Nov; 80(21): 6771-81.
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