RESEARCH PAPER
Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains
1
Department of Medical Biology, Medical University, Warsaw, Poland
2
Division of Nanobiotechnology, Faculty of Animal Sciences, University of Life Science, Warsaw, Poland
3
Department of Pharmaceutical Microbiology, Medical University, Warsaw, Poland
Corresponding author
Lidia Chomicz
Department of Medical Biology, Medical University of Warsaw, Nowogrodzka 73, 02-018, Warszawa, Poland
Department of Medical Biology, Medical University of Warsaw, Nowogrodzka 73, 02-018, Warszawa, Poland
Ann Agric Environ Med. 2019;26(1):198–202
KEYWORDS
Acanthamoeba keratitiscontact lens solutionssilver/gold nanoparticlesAcanthamoeba trophozoitesanti-amoebic in vitro effect
TOPICS
Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases)Prevention of occupational diseases in agriculture, forestry, food industry and wood industryWork-related accidents and injuries in agriculture, forestry, food industry and wood industry: incidence, causes, social aspects and preventionState of the health of rural communities depending on various factors: social factors, accessibility of medical care, etc.
ABSTRACT
Introduction:
Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease – Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites.
Material and methods:
Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25–2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line.
Results:
Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed.
Conclusions:
Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.
Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease – Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites.
Material and methods:
Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25–2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line.
Results:
Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed.
Conclusions:
Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.
REFERENCES (38)
1.
Lorenzo-Morales J, Khan NA, Walochnik J. An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment. Parasite. 2015; 22: 10. http://doi.org/10.1051/parasit....
2.
Walochnik J, Scheikl U, Haller-Schober EM. Twenty years of Acanthamoeba diagnostics in Austria. J. Eukaryot Microbiol. 2015; 62: 3–11. https://doi.org/10.1111/jeu.12....
3.
Martinez AJ, Visvesvara GS. Free-living, amphizoic and opportunistic amebas. Brain Pathol. 1997; 7: 583–598. https://doi.org/10.1111/j.1750....
4.
Padzik M, Hendiger EB, Szaflik JP, Chomicz L. Amoebae of the genus Acanthamoeba – pathological agents in humans. Post Mikrobiol. 2017; 56: 429–439.
5.
Chomicz L, Padzik M, Graczyk Z, Starosciak B, Graczyk TK, Naprawska A, Oledzka G, Szostakowska B. Acanthamoeba castellanii: In vitro effects of selected biological, physical and chemical factors. Exp Parasitol. 2010; 126: 103–5. https://doi.org/10.1016/j.expp....
6.
Trabelsi H, Dendana F, Sellami A, Sellami H, Cheikhrouhou F, Neji S, Makni F, Ayadi A. Pathogenic free-living amoebae: epidemiology and clinical review. Path Biol. 2012; 60: 399–405. https://doi: 10.1016/j.patbio.2012.03.002.
7.
Clarke B, Sinha A, Parmar DN, Sykakis E. Advances in the diagnosis and treatment of Acanthamoeba keratitis. J Ophthalmol. 2012; 484–892. https://doi.org/10.1155/2012/4....
8.
Lorenzo-Morales J, Martín-Navarro CM, López-Arencibia A, Arnalich-Montiel F, Piñero JE, Valladares B. Acanthamoeba keratitis: an emerging disease gathering importance worldwide?. Trends Parasitol. 2013; 29: 181–187. https://doi.org/10.1016/j.pt.2....
9.
Chomicz L, Conn DB, Padzik M, Szaflik JP, Walochnik J, Zawadzki PJ, Pawłowski W, Dybicz M. Emerging Threats for Human Health in Poland: Pathogenic Isolates from Drug Resistant Acanthamoeba Keratitis Monitored in terms of Their In Vitro Dynamics and Temperature Adaptability. BioMed Res Int. 2015; 231–285. https://doi.org/10.1155/2015/2....
10.
Niyyati M, Sasani R, Mohebali M, Ghazikhansari M, Kargar F, Hajialilo E, Rezaeian M. Anti-Acanthamoeba Effects of Silver and Gold Nanoparticles and Contact Lenses Disinfection Solutions. Iran J Parasitol. 2018; 13: 180–185.
11.
Padzik M, Chomicz L, Szaflik JP, Chruścikowska A, Perkowski K, Szaflik J. In vitro effects of selected contact lens care solutions on Acanthamoeba castellanii strains in Poland. Exp Parasitol. 2014; 145: 98–101. https://doi.org/10.1016/j.expp....
12.
Lonnen J, Kilvington S, Lam A, Heaselgrave W. Biocidal efficacy of multipurpose contact lens disinfectant solutions against Acanthamoeba species. Contact Lens Anterio. 2011; 34: 30. https://doi.org/10.1016/S1367-....
13.
Rzeszutek J, Matysiak M, Czajka M, Sawicki K, Rachubik P, Kruszewski M, Kapka–Skrzypczak L. Zastosowanie nanocząstek i nanomateriałów w medycynie. Hygeia Public Health. 2014; 49: 449–457.
14.
Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for gram-negative bacteria. J Colloid Interf Sci. 2004; 275: 177–182. https://doi.org/10.1016/j.jcis....
15.
Said DE, Elsamad LM, Gohar YM. Validity of silver, chitosan, and curcumin nanoparticles as anti-Giardia agents. Parasitol Res. 2012; 111: 545–554. https://doi: 10.1007/s00436-012-2866-1.
16.
Saad HA, Soliman MI, Azzam AM, Mostafa B. Antiparasitic activity of silver and copper oxide nanoparticles against Entamoeba histolytica and Cryptosporidium parvum cysts. J Egypt Soc Parasitol. 2015; 45: 593–602. https://doi.org/ 10.12816/0017920.
17.
Ullah I, Cosar G, Abamor ES, Bagirova M, Shinwari ZK, Allahverdiyev AM. Comparative study on the antileishmanial activities of chemically and biologically synthesized silver nanoparticles (AgNPs). 3 Biotech. 8. 2018; 98. https://doi.org/10.1007/s13205....
18.
Padzik M, Hendiger EB, Chomicz L, Grodzik M, Szmidt M, Grobelny J, Lorenzo-Morales J. Tannic acid modified silver nanoparticles as a novel therapeutic agent against Acanthamoeba. Parasitol Res. 2018; 117: 3519–3525. https://doi.org/10.100 7/s00436-018-6049-6.
19.
Aqeel Y, Siddiqui R, Anwar A, Shah MR, Khan NA. Gold Nanoparticle Conjugation Enhances the Antiacanthamoebic Effects of Chlorhexidine. Antimicrob Agents Chemother. 2015; 60: 1283–1288. https://doi.org/10.1128/AAC.01....
20.
Borase HP, Patil CD, Sauter IP, Rott MB, Patil SV. Amoebicidal activity of phytosynthesized silver nanoparticles and their in vitro cytotoxicity to human cells. FEMS Microbiol Lett. 2013; 345: 127–131. https://doi.org/10.1111/1574-6....
21.
Orłowski P, Tomaszewska E, Gniadek M, Baśka P, Nowakowska J, Sokołowska J, Nowak Z, Donten M, Celichowski G, Grobelny J, Krzyżowska M. Tannic acid modified silver nanoparticles show antiviral activity in herpes simplex virus type 2 infection. PloS one 2014; 9: 104–113. https://doi.org/10.1371/journa....
22.
Zielinska M, Sawosz E, Grodzik M, Wierzbicki M, Gromadka M, Hotowy A, Sawosz F, Lozicki A, Chwalibog A. Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis. Int J Nanomed. 2011; 6: 3163–3172. https://doi.org/10.2147/ijn.s2....
23.
McBride J, Ingram PR, Henriquez FL, Roberts CW. Development of colorimetric microtiter plate assay for assessment of antimicrobials against Acanthamoeba. J Clin Microbiol. 2005; 43: 629–634. https://doi.org/10.1128/jcm.43....
24.
Radford CF, Minassian DC, Dart JKG. Acanthamoeba keratitis in England and Wales: incidence, outcome, and risk factors. Br J Ophthalmol. 2002; 86: 536–542. https://doi.org/10.1136/bjo.86....
25.
Kal A, Toker MI, Kaya S. The comparison of antimicrobial effectiveness of contact lens solutions. Int Ophthalmol. 2016; 37: 1103–1114. https://doi.org/10.1007/s10792....
26.
Murthy SK. Nanoparticles in modern medicine: State of the art and future challenges. Int J Nanomed. 2007; 2: 129–141.
27.
Shrivastava S, Bera T, Roy A, Singh G, Ramachandrarao P, Dash D. Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology. 2007; 18: 103–225. https://doi.org/10.1088/0957-4....
28.
Grün AL, Scheid P, Hauröder B, Emmerlingand C, Manz W. Assessment of the effect of silver nanoparticles on the relevant soil protozoan genus Acanthamoeba. J Plant Nutr Soil SC. 2017; 180: 602–613. https://doi.org/10.1002/jpln.2....
29.
Willcox MDP, Hume EBH, Vijaya AK, Petcavichc R. Ability of silver-impregnated contact lenses to control microbial growth and colonisation. J Optom. 2010; 3: 143–148. https://doi.org/10.1016/S1888-....
30.
Anwar A, Siddiqui R, Hussain MA, Ahmed D, Shah MR, Khan NA. Silver nanoparticle conjugation affects antiacanthamoebic activities of amphotericin B, nystatin, and fluconazole. Parasitol Res. 2018; 117: 265–271. https://doi.org/10.1007/s00436....
31.
Bondarenko O, Juganson K, Ivask A, Kasemets K, Mortimer M, Kahru A. Toxicity of Ag, CuO and ZnO nanoparticles to selected environmentally relevant test organisms and mammalian cells in vitro: a critical review. Arch Toxicol. 2013; 87: 1181–1200. https://doi.org/10.1007/s00204....
32.
Lok CN, Ho CM, Chen R, He QY, Yu WY, Sun H, Tam PK, Chiu JF, Che CM. Proteomic analysis of the mode of antibacterial action of silver nanoparticles. Proteome Res. 5 (2006) 916–924. https://doi:org/ 10.1021/pr0504079.
33.
Yan X, He B, Liu L, Qu G, Shi J. Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: proteomics approach. Metallomics. 2018; 10: 557–564. https://doi.org/10.1039/c7mt00....
34.
Patil SV, Borase HP, Patil CD, Salunke BK. Biosynthesis of Silver Nanoparticles Using Latex from Few Euphorbian Plants and Their Antimicrobial Potential. Appl Biochem Biotechnol. 2012; 167: 776–790. https://doi.org/10.1007/s12010....
35.
Bazzaz BSF, Khameneh B, Jalili-Behabadi MM, Malaekeh-Nikouei B, Mohajeri SA. Preparation, characterization and antimicrobial study of a hydrogel (soft contact lens) material impregnated with silver nanoparticles. Contact Lens Anterio. 2014; 37: 149–152. https://doi.org/10.1016/j.clae....
36.
Amos CF, George MD. Clinical and laboratory testing of a silver-impregnated lens case. Contact Lens. Anterio. 2006; 29: 247–255. https://doi.org/10.1016/j.clae....
37.
Codling CE, Maillard JY, Russell AD. Aspects of the antimicrobial mechanisms of action of a polyquaternium and an amidoamine. J Antimicrob Chemother. 2003; 51: 1153–1158. https://doi.org/10.1093/jac/dk....
38.
Santodomingo-Rubido J, Mori O, Kawaminami S.Cytotoxicity and antimicrobial activity of six multipurpose soft contact lens disinfecting solutions. Ophthalmic Physiol Opt. 2006; 26: 476–482. https://doi.org/10.1111/j.1475....
Share
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.