Condition of mineralized tooth tissue in a population of 15-year-old adolescents living in a region of Ukraine with slightly exceeded fluorine concentration in the water
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Department of Conservative Dentistry and Endodontics, Medical University, Lublin, Poland
Department of Cultivation and Fertilization of Horticultural Plants, University of Life Sciences, Lublin, Poland
Danylo Halytsky National Medical University, Lviv, Ukraine
Corresponding author
Karolina Maria Pitura   

Department of Cultivation and Fertilization of Horticultural Plants, Leszczyńskiego 58, 20-068, Lublin, Poland
Ann Agric Environ Med. 2019;26(4):623-629
Fluorine is a common element in nature; however, the difference between a beneficial dose and a toxic dose for the organisms is small. The main source of fluoride for humans is water in addition to food.

The aim of this study was to estimate the degree of severity of pathological changes, namely, caries or fluorosis, in the mineralized tooth tissue of 15-year-old adolescents with respect to their hygienic and nutritional habits, and the content of fluorine in drinking water, soil and plant products.

Material and methods:
100 adolescents aged 15 from schools in Sosniłka, L’viv region, were examined. The condition of mineralized tooth tissue was evaluated by the caries severity, expressed by the mean number D3MFT, caries frequency and value of the SIC index. Fluorine in plant material and soil were determined according to the PN-G-04543:1982 standard, and water according to the PN-EN ISO 10304 – 1: 2009+ AC: 2012 standard.

Severity of caries disease expressed by the D3MFT number in the examined group of 15-year-olds was 3.39; in the group of girls – 3.08, and in the group of boys – 3.76. In the examined group, the average number of teeth with fluorosis was 7.59. Value of the SIC index among the examined population of students (n-31) was 6.26: 5.89 (n-18) for girls and 7.31 (n-13) for boys. Fluorine concentration in the water was 0.78 – 1.25(mg·dm-3). In the soil, it also did not vary across the sampled areas and amounted, on average, to 176 mg·dm-3. The biggest fluorine content noted in the dry mass of beetroots was 3.50 (mg F· kg), and the lowest – 3.34 (mg F· kg).

Close to optimal fluorine content lowers caries severity and frequency of fluorosis. Optimal fluorine content in drinking water and food does not require additional diet supplementation.

Palczewska-Komsa M, Kalisińska E, Szmidt M, Stogiera A. Fluorosis in humans and animals. Med Środow. 2015; 18(4): 57–61.
Harding MA, Omullanne DM. Water fluoridation and oral health. Acta Med Acad. 2013; 42(2): 131–139. doi: 10.5644/ama2006-124.81.
Bhagavatula P, Curtis A, Broffitt B, Weber-Gasparoni K, Warren JJ, Levy SM. The relationships between fluoride intake levels and fluorosis of late-erupting permanent teeth. J Public Health Dent. 2018; 78(2): 165–174. doi: 10.1111/jphd.12260.
Narsimha A, Sudarshan V. Data on fluoride concentration levels in semi-arid region of Medak, Telangana, South India. Data Brief. 2017; 6(16): 717–723. doi: 10.1016/j.dib.2017.11.089.
Skorowska-Zieleniewska J, Roszkowski W, Paprocka M. Fluorine in food – hygienic and nutritional problems and analytical. Metab. Fluoru. 1982; 15–19. (in Polish).
Boguszewska-Gutenbaum H, Turska-Szybka A, Remiszewski A. Dental Fluorosis – a Case Report. Nowa Stomatol. 2006; 11(2): 68–72.
Fawell J, Bailey K, Chilton J, Dahi E, Fewtrell L, Magara Y. London: IWA Publishers; Human health effects: Fluoride in drinking water WHO drinking-water quality series. 2006; p. 29–35.
Smolik B, Telesiński A, Szymczak J, Zakrzewska H. Assessing of humus usefulness in limiting of soluble fluoride content in soil. Ochr Środ Zasob Natur. 2011; 49: 202–208. (in Polish).
Khandare AL, Rao GS. Uptake of fluoride, aluminum and molybdenum by some vegetables from irrigation water. J Hum Ecol 2006; 19(4): 283–288.
Baunthiyal M, Ranghar S. Accumulation of fluoride by plants: potential for phytoremediation. Clean Soil Air Water. 2013; 43(1): 127–132.
Telesiński A, Śnioszek M, Bioindicators of environmental pollution with fluorine. Bromat. Chem. Toksykol. 2009; (4): 1148–1154.
Jańczuk Z, Kaczmarek U, Lipski M. Conservative dentistry and endodontics. Clinical outline. Wyd. Lekarskie. PZWL, Warszawa. 2014; p. 201–210, 504–505. (in Polish).
Msyamboza KP, Phale E, Namalika JM, Mwase Y, Samonte GC et al. Magnitude of dental caries, missing and filled teeth in Malawi. National oral health survey. BMC Oral Health. 2016; (9): 16–29. doi: 10.1186/s12903-016-0190-3.
Han J, Bao-Jun T, Du MQ, Wei H, Bin P. Study of dental caries and the influence of social-behavioral risk factors on dental caries of 1,080 15-year-old adolescents. East Mediter. Health J. 2009; 15(4): 993–1004.
Pondingo-Loyola AP, Medina-Solis CE, Borges-Yaňez SA, Pati No-Martin N, Islas- Márguez A, Maupone G. Prevalence and severity of dental caries in adolescents aged 12 and 15 living in communities with various fluoride concentrations. J. Public. Health Dent. 2007; 67 (1): 8–13.
El – Nadeef MA, Al Hussani E, Hassab H, Arab IA. National survey of the oral health of 12- and 15-year-old schoolchildren in the United Arab Emirates. East Mediter Health. 2009; 15 (4): 993–1004.
Hamissi J, Ramezani GH, Ghoduusia A. Prevalence of dental caries among high school attendees in Qazvin, Iran J Indian Soc Pedod Prev Dent. 2008; 26(2): 53–55.
Gorbatova MA, Gorbatova LN, Gribovski AM. Dental caries experience among 15-year-old adolescents in north-west Russia. Int J Circumpolar Health. 2007; 70(3): 232–235.
Milciuviene S, Bendoraitiene E, Andruskeviciene V, et al. Dental caries prevalence among 12–15-year-olds in Lithuania between 1983 and 2005. Medicine (Kaunas). 2009; 45(1): 68–76.
Doumit M, Doughan B. Dental caries and fluorosis among children in Lebanon. Indian J Dent Res. 2018; 29(3): 317–322.
Smolar N, Biezwuszko E, Czuhraj N, Dubiecka I, Szybinski W. Dental diseases of children inhabiting Lviv district. LNUM Lviv. 2012; p. 14–15.
Bachanek T, Chałas R, Zimenkovsky A, Hendzel B, Wolańska E, Samborski D, Pitura K et al. The content of chosen elements in drinking water and prevalence of caries and hygienic habits of 15-year-old youth living in Lviv (Ukraine) and Lublin (Poland). J Elem. 2018; 23(2): 745–756. doi: 10.5601/jelem. 2017.22.4.1425.
Oulis CJ, Tsinidou K, Vadiakas G, Mamai-Homata E, Polychronopouou A, Athanasouli T. Caries prevalence of 5, 12 and 15-year-old Greek children: A national pathfinder survey. Community Dent Health. 2012; 29(1): 28–32.
Sgan-Cohen HD, Margvelashvili V, Kalandadze M, Gordon M, Margvelashvili M, Zini A. Dental caries among children in Georgia by age, gender, residence location and ethnic group. Community Dent Health. 2014; 31(3): 163–166.
Goyal A, Gau K, Chwala HS, Kaur M, Kapur A. Epidemiology of dental caries in Chandigarh school children and trends over the last 25 years. J Indian Soc Pedod Prev Dent. 2007; 25(3): 115–118.
Ambrakova V, Jankulovska M, Arian D, Glavina D, Soleva A. Dental caries experience among secondary school children in the Vardar region of the Republic of Macedonia. Oral Health Dent Manag. 2014; 13(3): 805–810.
Results of epidemiological studies conducted in the programme “Monitoring of health of the oral cavity in 2011”. Ministry of Health 2011. content/uploads/2014/11/monitoring_ju_2011.pdf.
Hilt A, Rybarczyk-Townsend E, Wochna-Sobańska M. Dental status of junior high school students in Łódzkie voivodeship. Przegl Epidemiol. 2014; 68(1): 59–64.
Meler J, Meler G. Fluidation of drinking water – advantages and disadvantages. J Elementol. 2006; 11 (3): 379–387.
Akosu TJ, Zoakah AL, Chirdan OA. The prevalence and severity of dental fluorosis in the high and low altitude parts of Central Plateau, Nigeria. Community Dent Health. 2009; 26(3): 138–142.
Jędra M, Sawilska-Rautenstrauch D, Gawarska H, Starski A. Fluorine content in total diets samples of small children in Poland. Rocz PZH. 2011; 3(62): 275–281.
Carvalho TS, Kehrle HM, Sampaio FC. Prevalence and severity of dental fluorosis among students from João Pessoa, PB, Brazil. Braz Oral Res. 2007; 21(3): 198–203. doi: 10.1590/S1806-8324200700030000.
Momeni A, Neuhäuser A, Ronner N, Heinzel-Gutenbrunner M, Abou-Fidah J, et al. Prevalence of dental fluorosis in german schoolchildren in areas with different preventive programmes. Caries Res. 2007; 41(6): 437–444.
Brandao IM, Peres AS, Saliba NA, Moimaz SA. Prevalence of dental fluorosis in school children from Marinópolis, São Paulo. Cad Saude Publica. 2002; 18(3): 877–881.
Rustagi N, Radhore AS, Meena JK, Chugh A, Pal R. Neglected health literacy undermining fluorosis control efforts: a pilot study among schoolchildren in an endemic village of rural Rajasthan, India. J Family Med Prim Care. 2017; 6(3): 533–537.
Kadir RA, Al-Maqutari RA. Endemic fluorosis among 14-year-old Yemeni adolescents: An exploratory survey. Int Dent J. 2010; 60(6): 407–410.
Molina-Frechero N, Gaona E, Angulo M, Sánchez-Pérez L, Gonzlez González R, Nevárez Rascón M, et al. Fluoride exposure effects and dental fluorosis in children in Mexico city. Med Sci Monit. 2015; (21): 3664–3670. doi: 10.12659/msm.895351.
Rames M, Narasimhan M, Krishnan R, Aruna RM, Kuruvilla S. The effect of fluorosis on human teeth under light microscopy: a cross-sectional study. J Oral Maxillofac Pathol. 2017; 21(3): 345–350.
Naidu GM, Rahamthullah SA, Kopuri RK, Kumar YA, Suman SV, Balagna RN. Prevalence and self-perception of dental fluorosis among 15 year old school children in Prakasham district of South India. J Int Oral Health. 2013; 5(6): 67–71.
Sudhir KM, Prashant GM, Subba Reddy VV, Mohandas U, Chandu GN. Prevalence and severity of dental fluorosis among 13- to 15-year-old school children of an area known for endemic fluorosis: Nalgonda district of Andhra Pradesh. J Indian Soc Pedod Prev Dent. 2009; 27(4): 190–196.
Salas-Pereira MT, Beltran-Aquilar ED, Chavarria P, Solórzano I, Horowitz H. Enamel fluorosis in 12- and 15-year-old school children in Costa Rica. Results of a national survey, 1999. Community Dent Health. 2008; 25(3): 178–184.
De Moura MS, Barbosa PR, Nunes dos Santos DL, Dantas-Neta NB, Moura de F, de Lima MDE. Epidemiological surveillance of dental fluorosis in a city with a tropical climate with a fluoridated public drinking water supply. Cien Saude Colet. 2016; 21(4): 1247–1254. doi: 10.1590/1413-81232015214.13852015.
Wiener RC, Shen C, Findley P, Tan X, Sambamoorhi U. Dental fluorosis over time: a comparison of national health and nutrition examination survey data from 2001–2002 and 2011–2012. Hyg. 2018; 92(1): 23–29.
De Lourdes Azpeitia-Valadez M, Sánchez-Hernández MA, Rgriquez-Frausto M. Risk factors for dental fluorosis in children between 6 and 15 years old. Rev Med Inst Mex Sequero Soc. 2009; 47(3): 265–270.
Celeste RK, Luz PB. Independent and Additive Effects of Different Sources of Fluoride and Dental Fluorosis. Pediatr. Dent. 2016; 38(3): 233–238.
Verna A, Shett BK, Guddattu V, Chourasia MK, Pundir P. High prevalence of dental fluorosis among adolescents is a growing concern: a school based cross- sectional study from Southern India. Environ Health Prev Med. 2017; 22(1): 17. doi: 10.1186/s12199-017-0624-9.
Al-Akwa AA, Al-Maweri. Dental caries prevalescence and its association with fluoride level in drinking water in Sanaa, Yemen. Eur. J. Dent. 2018; 12(1): 15–20.
Firempong C, Nsiah K, Awunyo-Vitor D, Dongsogo J. Soluble fluoride levels in drinking water-a major risk factor of dental fluorosis among children in Bongo community of Ghana. Ghana. Med J. 2013; 47(1): 16–23.
Okibe FG, Ekanem EJ, Paul ED, Shallangwa GA, Ekwumemgbo PA, Sallau MS, Abanka OC. Fluoride content of soil and vegetables from irrigation farms on the bank of river Galma, Zaria, Nigeria. Australian J Basic Applied Sci. 2010; 4(5): 779–784.
Kulczycki G, Spiak Z, Kamińska A. Impact of Chemical Company „Wizów” on the content of fluorine in soil and plant. Zesz Nauk UP Wrocław. Rolnictwo. 2006; LXXXIX (546): 243–248.
Kłódka D, Musik D, Wójcik K, Telesiński A. Fluorine content in selected vegetables grown within the area affected by emission of that element from the “POLICE”. Bromat Chem Toksykol. 2008; XLI (4): 964–969.
Kusa Z, Wardas W, Sochacka J, Pawłowska-Góral K. Fluoride Accumulation in Selected Vegetables During Their Vegetation. Pol J Environ Stud. 2004; 13(1): 55–58.
Stogiera A, Buczkowska-Radlińska J. Anthropogenic sources of fluorine – the impact on the environment and human health – a literature review. Dental Forum 2014; XLII: 57–62. doi: 10.20883/ issn.1732-0801.
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