Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines
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Muğla Sıtkı Koçman University, Mugla, Turkey
Adanan Menderes University, Aydin, Turkey
Ege University, Izmar, Turkey
Corresponding author
Nur Ceyhan Güvensen   

Muğla Sıtkı Koçman University
Ann Agric Environ Med. 2019;26(2):290-297
Introduction and objective:
Since antiquity, C. babylonica (L.) L. extracts has been used as a remedy for primary health care in traditional medicine. In this study, a total of seven different crude extracts (acetone, chloroform, hexane, ethylacetate, methanol, ethanol and water) from branches and leaves of C. babylonica (L.) L. were prepared to determine antimicrobial and antiproliferative activity against cancer cell lines.

Material and Methods:
MIC assay was used for antimicrobial activity against gram positive and gram negative bacteria, and one yeast. MTT assay was applied to screen the antiproliferative activity of seven extracts, and to determine dose- and time-dependent effects of the aceton extract on A549, PC-3, MCF-7, and HeLa cell lines.

The aceton extract of C.babylonica (L.) L. showed the best antibacterial activity against Bacillus cereus, P. aeruginosa and C. albicans (MIC: 1.6 mg/mL). GC-MS analyses allowed six compounds to be determined; the main constituents of acetone extract from C. babylonica (L.) L. were diacetone alcohol (53.47 %), 1-dexadecene (10.19 %) and 1-tetradecene (8.67 %). In addition, seven different solvent extracts at 500 µg/mL caused antiproliferative activity between 84% – 88%, compared to control. Dose-dependent effects of the extracts on A549 cells indicated that chloroform, ethyl acetate, and aceton extract were the most effective extracts with the IC50 values of 9, 33, and 36 µg/mL, respectively.

The results clearly demonstrate that C. babylonica (L.) L. exhibited a strong antimicrobial effect and antiproliferative activity against cancer cells in vitro. Further studies are required to isolate and characterize the active pure compounds responsible for the antimicrobial and antiproliferative activities.

Davies J, Davies D. Origins and evolution of antibiotic resistance. Microbiol Mol Biol Rev. 2010; 74(3): 417–33.
Silva NCC, Fernandes Júnior A. Biological properties of medicinal plants: a review of their antimicrobial activity. J Venom Anim Toxins Inc Trop Dis. 2010; 16(3): 402–413.
Dagdelen A. Identifying antioxidant and antimicrobial activities of the phenolic extracts and mineral contents of virgin olive oils (Olea europaea L. cv. Edincik Su) from different regions in Turkey. J Chemistry. 2016: 1–11.
Khazir J, Mir BA, Pilcher L, Riley DL. Role of plants in anticancer drug discovery. Phytochemistry Letters. 2014; 7:173–181.
Prakash O, Kumar A, Kumar P, Ajeet. Anticancer Potential of Plants and Natural Products: A Review. Am J Pharmacol Sci. 2013; 1(6): 104–115.
Hellwig FH. Centaureinae (Asteraceae) in the Mediterranean – history of ecogeographical adiation. Plant Syst Evol. 2004; 246: 137–162.
Kultur S, Bona M, Ozdemir Naht E. A new species of Centaurea (Asteraceae) from East Anatolia, Turkey. Phytotaxa. 2015; 247(1): 085–091.
Bona M. An overview to Centaurea s.l. (Asteraceae) based on herbarium specimens of ISTE. J Fac Pharm. 2013; 43(2): 121–137.
Baytop T. Turkiye’de Bitkilerle Tedavi. Nobel Tıp Kitapevi, İstanbul. 1999.
Tuzlacı E, Isbilen DFA, Bulut G. Turkish Folk Medicinal Plants, VIII:Lalapaşa (Edirne). Marmara Pharm J. 2010; 14: 47–52.
Skaltsa H, Lazari D, Panagouleas C, Georgiadou E, Garcia B, Sokovic M. Sesquiterpene lactones from Centaurea thessala and Centaurea attica. Antifungal activity. Phytochem. 2000; 55: 903–908.
Karioti A, Skaltsa H, Lazari D, Sokovic M, Garcia B, Harvala C. Secondary metabolites from Centaurea deusta with antimicrobial activity. Z Naturforsch. 2002;57c: 75–80.
Kumarasamy Y, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of Scottish plants for antibacterial activity. J Ethnopharmacol. 2002; 83: 73–77.
Kumarasamy Y, Middleton M, Reid RG, Nahar L, Sarker SD. Biological activity of serotonin conjugates from the seeds of Centaurea nigra. Fitoterapia. 2003; 74: 609–612.
Barbour EK, Sharif MA, Sagherian VK, Habre AN, Talhouk RS, Talhouk SN Screening of selected indigenous plants of Lebanon for antimicrobial activity. J Ethnopharmacol: 2004; 93: 1–7.
Yayli N, Yasar A, Guec C, Usta A, Kolayli S, Composition and antimicrobial activity of essential oils from Centaurea sessilis and Centaurea armena. Phytochem. 2005; 66, 1741–1745.
Skliar MI, Toribio MS, Oriani DS. Antimicrobial activity of Centaurea diffusa . Fitoterapia. 2005; 76(7): 737–739.
Guven K, Celik S, Uysal I. Antimicrobial activity of Centaurea species. Pharm Biol. 2005; 43(1): 67–71.
Buruk K., Sokmen A, Aydin F, Erturk M. Antimicrobial activity of some endemic plants growing in the Eastern Black Sea Region, Turkey. Fitoterapia. 2006; 77(5): 388–391.
Karamenderes C, Khan S, Tekwani BL, Jacob MR, Khan IA. Antiprotozoal and antimicrobial activities of Centaurea L. species growing in Turkey. Pharm Biol. 2006; 44(7): 534–539.
Ugur A, Duru ME, Ceylan O, Sarac N, Varol O, Kivrak I. Chemical composition, antimicrobial and antioxidant activities of Centaurea ensiformis Hub.-Mor. (Asteraceae), an endemic species to Mugla (Turkey). Nat Product Res. 2009; 23(2): 149–167.
Barrero AF, Oltra JE, Alvarez M, Raslan DS, Saude D, Akssira M. New sources and antifungal activity of sesquiterpene lactones. Fitoterapia. 2000; 71: 60–64.
Koukoulitsa C, Geromichalos GD, Skaltsa H. VolSurf analysis of pharmacokinetic properties for several antifungal sesquiterpene lactones isolated from Greek Centaurea sp. J Comput Aid Mol Des. 2005; 19(8): 617–623.
Shoeb M, MacManus SM, Jaspars M, Trevidu J, Nahar L, Kong-Thoo-Lin, P. Montamine, a unique dimeric indole alkaloid, from the seeds of Centaurea montana (Asteraceae), and it’s in vitro cytotoxic activity against the CaCo 2 colon cancer cells. Tetrahedron. 2006; 62(48): 11172–11177.
Medjroubi K, Benayache F, Bermejo J. Sesquiterpene lactones from Centaurea musimomum. Antiplasmodial and cytotoxic activities. Fitoterapia. 2005; 76 (7,8): 744–746.
Garbacki N, Gloaguen V, Damas J, Bodart P, Tits M, Angenot L. Anti-inflammatory and immunological effects of Centaurea cyanus flower-heads. J Ethnopharmacol. 1999; 68: 235–241.
Dündar E, Çiftçi GA, Altıntaş A. Anticancer activity of Centaurea babylonica L. Proceedings 2017; 1(10): 1043.
Sen A, Bitis L, Birteksoz-Tan S, Bulut G. In vitro evaluation of antioxidant and antimicrobial activities of some Centaurea L. Species. Marmara Pharm J. 2013; 17: 42–45.
Clinical and Laboratory Standards Institute (CLSI). Methods for Dilution Antimicrobial Susceptibility Test for Bacteria that Grow Aerobically; Approved Standard M7-A 6th edn. National Committee for Clinical Laboratory Standards, 2003; Wayne, Philadelphia.
Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing. 16th Informational Supplement M100-S16. National Committee for Clinical Laboratory Standards, 2006; Wayne, Philadelphia.
Adams RP. Identification of essential oil components by gas chromatography/masss spectrometry. 1995; Allured Publishing: Carol Stream, IL.
Holst-Hansen C, Brünner N. MTT Cell Proliferation assay, Cell Biology: a Laboratory Handbook. San Diego Academic Press. 1998;16–18.
Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod. 2007; 70(3): 461–477.
Khammar A, Djeddi S. Pharmacological and biological properties of some Centaurea species. Eur J Sci Res. 2012; 84(3): 398–416.
Cansaran A, Dogan NM, Oztekin M, Acar G. Antimicrobial activity of various extracts of Centaurea cankiriense A. Duran and H. Duman. Afr J Microbiol Res. 2010; 4: 608–612.
Köse YB., Iscan G, Demirci B, Baser KHC, Celik S. Antimicrobial activity of the essential oil of Centaurea aladagensis. Fitoterapia. 2007; 78(3): 253–254.
Senatore F, Formisano C, Rao A, Bellone G, Bruno M. Volatile components from flower-heads of Centaurea nicaeensis All., C. parlatoris Helder and C. solstitialis L. ssp. schouwii (DC.) Dosta´l growing wild in southern Italy and their biological activity. Nat Prod Res. 2008; 22: 825–832.
Panagouleas C, Skaltsa H, Lazari D, Skaltsounis A, Sokovic A. Antifungal activity of secondary metabolites of Centaurea raphanina ssp. mixta, growing wild in Greece. Pharm Biol. 2003; 41: 266–270.
Zater H, Huet J, Fontaine V, Benayache, Stevigny C, Duez P, Benayache F. Chemical constituents, cytotoxic, antifungal and antimicrobial properties of Centaurea diluta Ait. subsp. algeriensis (Cross.& Dur.) Maire. Asian Pac J Trop Med. 2016; 9: 554–561.
Erol-Dayi O, Pekmez M, Bona M, Aras-Perk A, Arda N. Total phenolic contents, antioxidant activities and cytotoxicity of three Centaurea species: C. calcitrapa subsp. calcitrapa, C. ptosimopappa and C. spicata. Free Rad and Antioxidan. 2011; 1: 31–36.
Sekerler T, Sen A, Bitis L, Sener A. Anticancer, Antioxidant and Anti-Inflammatory Activities of Chloroform Extracts from Some Centaurea Species. Proceedings. 2018; 2: 1542.
Artun FT, Karagoz A, Ozcan G, Melikoglu G, Anil S, Kultur S, Sutlupina N. In vitro anticancer and cytotoxic activities of some plant extracts on HeLa and Vero cell lines. JBUON. 2016; 21(3): 720–725.
Lazutka JR, Mierauskiene J, Slapsyte G, Dedonyte V. Genotoxicity of dill (Anethum graveolens L.), peppermint (Menthax piperita L.) and pine (Pinus sylvestris L.) essential oils in human lymphocytes and Drosophila melanogaster. Food Chem Toxicol. 2001; 39: 485–492.
Köse YB, Altıntaş A, Demirci B, Çelik S, Can Başer KH. Composition of the essential oil of endemic Centaurea paphlagonica (Bornm.) wagenitz from Turkey. Asian J Chem. 2009; 21(3): 1719–1724.
Belgaty AM. Chemical composition and cytotoxic activity of Centaurea scopareasieb against four human cell lines. J Pharm Sci Res. 2015; 7(3): 103–107.
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