Tawny owl Strix aluco as a potential transmitter of Enterobacteriaceae epidemiologically relevant for forest service workers, nature protection service and ornithologists
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Department of Zoology, Animal Ecology and Wildlife Management, University of Life Science, Lublin, Poland
Department of Small Ruminants Breeding and Agriculture Advisory, University of Life Science, Lublin, Poland
Independent Laboratory for Cell Metabolism, Institute of Rural Health, Lublin, Poland
Department of Landscape Ecology and Nature Conservation, University of Life Science in Lublin, Poland
Wiktor Bojar   

Department of Small Ruminants Breeding and Agriculture Advisory, University of Life Science, Lublin, Poland
Ann Agric Environ Med. 2017;24(1):62–65
Established taxa within the Enterobacteriaceae wereisolated from cloacal swabs of Strix aluco chicks in nest boxes located at five research sites. ChromID ESBL medium (bioMerieux) was used to select a pool of Enterobacteriaceae strains producing extended-spectrum beta-lactamases. Drug sensitivity of the chosen strains was determined from the full pool of Enterobacteriaceae to 6 chemotherapeutics of different mechanisms of action. The study evaluated the sensitivity of ESBL-synthesizing isolates to substances belonging to penicillins, cephalosporins, cephamycins, clavams, carbapenems and monobactams. Analysis of the results indicated a potential role of Strix aluco in the dissemination of epidemiologically-relevant Enterobacteriaceae, and, importantly, pose health risks to forest service workers, nature protection service and ornithologists. The results can also serve as the basis for further environmental studies.
Cios Sz, Grzywaczewski G. Importance of selected factors influencing the size of tawny owl Strix aluco territories in the forests of Lublin region. Sylwan 2013; 157(5): 348–357.
Wiącek J, Polak M, Grzywaczewski G. The Role of Forest Age, Habitat Quality, Food Resources and Weather Conditions for the Tawny Owl Strix aluco Populations. Polish J of Environ Stud. 2010; 19(5): 1039–1043.
Cramp S. (editor). Handbook of the Birds of Europe the Middle East and North Africa Vol 4. Oxford, New York, Oxford University Press, 1985.
Nowakowski G, Kochańska-Dziurowicz A, Widala E. Leptospirosis tick – Lyme disease. Przegląd Lekarski. 2000; 57(7–8): 424–426 (in Polish).
Romankow J, Kusiak W.Risk of tick- borne diseases in forestry workers. Nowiny Lek. 2004; 73(6): 454–458 (in Polish).
Dutkiewicz J, Śpiewak R, Jabłoński L, Szymańska J. Occupational biological risk factors – classification exposed occupational groups, measurement, prevention. Lublin, Ad punctum. 2007; (in Polish).
Cherubin CE, Eng RHK, Smith SM. Cephalosporin therapy for salmonellosis-Questions of efficacy and cross resistance with ampicillin. Arch Intern Med. 1986; 146: 2149–2152.
Karlowsky JA, Jones ME, Thornsberry C, Friedland IR, Sahm DF. Trends in Antimicrobial Susceptibilities among Enterobacteriaceae Isolated from Hospitalized Patients in the United States from 1998 to 2001,Antimicrob Agents Chemother. 2003; 47(5): 1681–1688.
Gupta R, Rauf SJ, Singh S, Smith J, Agraharkar ML. Sepsis in a renal transplant recipient due to Citrobacter braakii. Southern Medical Journal. 2003; 96(8): 796–798.
Bush K, Jacoby GA, Medeiros AA. A functional classification scheme for β-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother. 1995; 39: 1211–1233.
Livermore DM.β-lactamases in laboratory and clinical resistance. Clin Microbiol Rev. 1995; 8: 557–584.
Leclerc H, Mossel DAA, Edberg SC, Struijk CB. Advances in the Bacteriology of the Coliform Group: Their Suitability as Markers of Microbial Water Safety. Annu Rev Microbiol. 2001; 55: 201–34.
Pepperell C, Kus JV, Gardam MA, Humar A, Burrows LL. Low-Virulence Citrobacter Species Encode Resistance to Multiple Antimicrobials; 2002; 46(11): 3555–3560.
Bhatt BD, Zuckerman MJ, Foland JA, Disseminated Salmonella arizonae infection associated with rattlesnake meat ingestion. Am J Gastroenterol. 1989; 84: 433–435.
Chiodini RJ, Sundberg JP. Salmonellosis in reptiles: A review. Am J Epidemiol. 1981; 113: 494–499.
Gryz J, Krauze-Gryz D.The use of nest boxes by owls Strix aluco in central Poland. Studia i Materiały CEPL. 2011; 27: 120–125 (in Polish).
Stefaniuk E, Herda M, Kozińska A, Hryniewicz W. Chromogenic substrate chromID ESBL bioMérieux in monitoring hospital infections. Wyd bioMérieux Polska. 2008 (in Polish).
Kotra LP, Mobashery S. β-Laktam antibiotics, β-lactamases and bacterial resistance. Bull. Inst. Pasteur. 1998; 96: 139–150.
Bradford PA. Extended-spectrum β-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev. 2001; 14: 933–95.
Essack SY. The Development of β-Laktam Antibiotics in Response to the Evolution of β-Lactamases. Pharmaceutical Research. 2001; 18(10): 1391–1399.
Jakoby GL, Sutton L. β-lactamases and β-lactam resistance in Escherichia coli. Antimicrob Agents Chemother. 1985; 28: 703–705.
Chen ST, Clowes RC. Variations between the nucleotide sequence of Tn1, Tn2, and Tn3 and expression of β-lactamase in Pseudomonas aeruginosa and Escherichia coli. J Bacteriol. 1987; 169: 913–916.
Shah AA, Hasan F, Ahmed S, Hameed A. Characteristics, epidemiology and clinical importance of emerging strains of Gram-negative bacilli producing extended-spectrum β-lactamases. Research in Microbiology. 2004; 155: 409–421.
Thomson KS, Smith Moland E. Version 2000: the new β-lactamases of Gram-negative bacteria at the dawn of the new millennium. Microbes and Infection. 2000; 2: 1225−1235.
Kowalczyk-Pecka D, Puchalski A. Potential interaction between the Cepaea nemoralis wild snail and Citrobacter spp. bacteria. Vet Med. 2008; 64(6): 786–790.