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Division of methods for counting helminths’ eggs and the problem of efficiency of these methods

Jacek Karamon 4,  
Jacek Malicki 3,  
 
1
Institute of Agrophysics, Polish Academy of Sciences, Lublin, Poland
2
Department of Biological Health Hazards and Parasitology, Institute of Rural Health, Lublin, Poland
3
Department of Water Supply and Wastewater Disposal, Faculty of Environmental Engineering, Lublin University of Technology, Lublin, Poland
4
Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, Pulawy, Poland
Ann Agric Environ Med 2017;24(1):1–7
KEYWORDS:
ABSTRACT:
From the sanitary and epidemiological aspects, information concerning the developmental forms of intestinal parasites, especially the eggs of helminths present in our environment in: water, soil, sandpits, sewage sludge, crops watered with wastewater are very important. The methods described in the relevant literature may be classified in various ways, primarily according to the methodology of the preparation of samples from environmental matrices prepared for analysis, and the sole methods of counting and chambers/instruments used for this purpose. In addition, there is a possibility to perform the classification of the research methods analyzed from the aspect of the method and time of identification of the individuals counted, or the necessity for staining them. Standard methods for identification of helminths’ eggs from environmental matrices are usually characterized by low efficiency, i.e. from 30% to approximately 80%. The efficiency of the method applied may be measured in a dual way, either by using the method of internal standard or the ‘Split/Spike’ method. While measuring simultaneously in an examined object the efficiency of the method and the number of eggs, the ‘actual’ number of eggs may be calculated by multiplying the obtained value of the discovered eggs of helminths by inverse efficiency.
CORRESPONDING AUTHOR:
Katarzyna Jaromin-Gleń   
Institute of Agrophysics, Polish Academy of Sciences, Lublin, Poland
 
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