RESEARCH PAPER
Contamination of wheat grain with microscopic fungi and their metabolites in Poland in 2006–2009
 
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Department of Chemistry, Poznan University of Life Sciences, Poland
CORRESPONDING AUTHOR
Kinga Stuper-Szablewska   

Department of Chemistry, Poznan University of Life Sciences, Poland
 
Ann Agric Environ Med. 2014;21(3):504–509
KEYWORDS
ABSTRACT
Microscopic fungi are microorganisms commonly found in cereal products. Pathogens of cereals colonising kernels are responsible, among other things, for deterioration of the technological value of grain. However, the greatest threat is posed by mycotoxins produced by toxin-forming strains of these microorganisms. The aim of the present study was to determine the level of contamination with microscopic fungi and mycotoxins from the group of trichothecenes in wheat grain from Poland in a 4-year cycle. In the period 2006–2009, studies were conducted on the content of fungal metabolites (ergosterol [ERG] and type A and B trichothecenes) and the content of microscopic fungi expressed in colony-forming units (CFU) in wheat grain. A total of 129 grain samples were examined. Analysed wheat samples had similar contents of both the investigated fungal metabolites and levels of microscopic fungi. Contents of microscopic fungi were low. Concentration of ERG, on average, was 2.64 mg/kg, while in colony forming units this value ranged from 101 CFU/g to over 103 CFU/g. The total concentration of type A and B trichothecenes was also low and within the 4 years of the investigation did not exceed 0.062 mg/kg. Concentration of DON did not exceed 1,250 µg/kg, established as safe in grain for human consumption, in any of the tested samples. For the results collected in the years 2006–2009 and presented in this paper, correlations were calculated between the amount of mycoflora and analysed metabolites in 3 possible combinations: 0.7096 for ERG/total toxin concentration, 0.6086 for ERG/log CFU/g, and 0.4016 for the concentration of total toxins/log CFU/g. Highly significant correlations between the content of trichothecenes and the concentration of ERG indicate that the level of this metabolite is closely related to the content of mycotoxins in grain.
 
REFERENCES (25)
1.
Murray LF, Moss R. Estimation of fat acidity in milled wheat products. Part I. Colorimetric determination. J. Cereal Sci. 1990; 11: 171–177.
 
2.
Gil A, Ortega RM, Maldonado J. Wholegrain cereals and bread: a duet of the Mediterranean diet for the prevention of chronic diseases. Pub Health Nut. 2011; 14: 2316–2322.
 
3.
Stuper K, Kawka A, Buśko M, Suchowilska E, Szwajkowska-Michałek L, Matysiak A, Wiwart M, Perkowski J. The effect of mycoflora and trichothecene contents in bread wheat on quality of its milling products. Wroclaw University of Life Science Academic Publisher, Wroclaw, Poland, 2009.pp.96–102.
 
4.
Lancova K, Hajslova J, Kostelanska M, Kohoutkova J, Nedelnik J, Moravcova H, Vanova M. Fate of trichothecene mycotoxins during the processing: Milling and baking. Food Addit Cont. 2008; 25: 650–659.
 
5.
Buśko M, Perkowski J, Wiwart M, Góral T, Suchowilska E, Stuper K, Matysiak A, Szwajkowska-Michałek L. Distribution of fungal biomass in spike of naturally contaminated and inoculated with Fusarium culmorum. Cereal Res. Commu. 2008; 36: 477–479.
 
6.
Perkowski J, Wiwart M, Stuper K, Buśko M, Matysiak A, Suchowilska E. Concentration of fungal biomass and trichothecenes in different parts of einkorn. Ann Agric Environ Med. 2011; 18: 111–119.
 
7.
Yli-Mattila T. Ecology and evolution of toxigenic Fusarium species in cereals in northern Europe and Asia. J Plant Pathol. 2010; 92: 7–18.
 
8.
Gawrysiak-Witulska M, Wawrzyniak J, Ryniecki A, Perkowski J. Relationship of ergosterol content and fungal contamination and assessment of technological quality of malting barley preserved in a metal silo using the near-ambient method. J Stored Prod Res. 2008; 44: 360–365.
 
9.
Perkowski J, Kiecana I, Kaczmarek Z. Natural occurrence and distribution of Fusarium toxins in contaminated barley cultivars. Eur J Plant Pathol. 2003; 109: 331–339.
 
10.
Perkowski J, Buśko M, Stuper K, Kostecki M, Matysiak A, Szwajkowska-Michałek L. Concentration of ergosterol in small-grained naturally contaminated and inoculated cereals. Biologia 2008; 63: 542–547.
 
11.
Baliukoniene V, Bakutis B, Stankevicius H. Mycological and mycotoxicological evaluation of grain. Ann Agric Environ Med. 2003; 10: 223–227.
 
12.
Neagu C, Tofan C. Cereal contamination with toxinogenic moulds. J Agro Proces Tech. 2008; 14: 237–324.
 
13.
Krysińska-Traczyk E, Kiecana I, Perkowski J, Dutkiewicz J. Levels of fungi and mycotoxins in samples of grain and grain dust collected on farms in eastern Poland. Ann Agric Environ Med. 2001; 8: 269–274.
 
14.
Stuper K, Buśko M, Matysiak A, Perkowski J. Zanieczyszczenie zbóż grzybami mikroskopowymi oraz ich metabolitami zebranych na terenie Wielkopolski. ABiD 2010; 4: 89–96. (in Polish).
 
15.
Stuper K, Buśko M, Perkowski J. Rozwój chemicznych metod oznaczania zawartości mikroflory grzybowej w produktach zbożowych. ABiD 2008; 4: 68–74 (in Polish).
 
16.
Keblys M, Flyen A, Langseth W. Changes in grain production, mechanisms for sale of grain and possible effects on grain quality in Lithuania in the period 1990–1999. Acta Agricul Scan. 2000; 50: 97–101.
 
17.
Garaleviciene D, Pettersson H, Agnedal M. Occurrence of trichothecenes, zearalenone and ochratoxin A in cereals and mixed feed from central Lithuania. Mycotox Res. 2002; 18: 77–89.
 
18.
Šliková S, Šudyová V, Gregová E. Deoxynivalenol in wheat from the growing areas of Slovakia. Cereal Res Commun. 2008; 36: 279–287.
 
19.
Schothorst K, van Egmond D. Report EC SCOOP TASK 3.2.10. Collection of occurrence data of Fusarium toxins in food and assessment of dietary intake by the population of EU Member States 2004. http://ec.europa. eu/food/fs/scoop/task3210.pdf (access: 2013.03.15).
 
20.
Champeil A, Doré T, Fourbet JF. Fusarium head blight: epidemiological origin of the effects of cultural practices on head blight attacks and production of mycotoxins by Fusarium in wheat grains. Plant Sci. 2004; 166: 1389–1415.
 
21.
Nicholson P, Simpson DR, Wilson AH, Chandler E, Thomsett M. Detection and differentiation of trichothecene- and enniatin-producing Fusarium species on small grain cereals. Eur J Plant Pathol. 2004; 110: 503–514.
 
22.
Čoncovă E, la Ciakova A, Styriak I, Czerwiecki L, Wilczynska G. Fungal contamination and the levels of mycotoxins (DON and OTA) in cereal samples from Poland and East Slovakia. Czech J Food Sci. 2006; 24: 33–40.
 
23.
Váňová M, Klem K, Matušinský P, Trnka M. Prediction model for deoxynivalenol in wheat grain based on weather conditions. Plant Prot Sci. 2009; 45: 133–137.
 
24.
Wiśniewska H, Buśko M. Evaluation of spring wheat resistance to Fusarium seedling blight and head blight. Biologia 2005; 60: 287–293.
 
25.
Saxena J, Munimbazi C, Bullerman L.B. Relationship of mould count, ergosterol and ochratoxin A production. Int J Food Microbiol. 2001; 71: 29–34.
 
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