ORIGINAL ARTICLES

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Ann Agric Environ Med 1998, 5, 25-30

ELISA COMPARISON OF THREE WHOLE-CELL ANTIGENS OF
BORRELIA BURGDORFERI SENSU LATO IN SEROLOGICAL STUDY OF DOGS
FROM AREA OF KOŠICE, EASTERN SLOVAKIA

Astéria Štefančíková1, Gabriela Tresová1, Branislav Peťko1, Ildiko Škardová2, Edina Sesztáková2

1Parasitological Institute of the Slovak Academy of Sciences, Košice, Slovak Republic
2University of Veterinary Medicine, Košice, Slovak Republic

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Štefančíková A, Tresová G, Peťko B, Škardová I, Sesztáková E: Elisa comparison of three whole-cell antigens of Borrelia burgdorferi sensu lato in serological study of dogs from area of Košice, eastern Slovakia.. Ann Agric Environ Med 1998, 5, 25-30.

Abstract: Three antigens of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto - Slovak strain Ir 105, B. garini - Slovak strain K 24 and B. burgdorferi sensu stricto - American strain B 31) were compared by ELISA on a group of dogs from urban agglomeration of Košice, eastern Slovakia. Of 256 serum samples from dogs examined for the presence of anti-Borrelia IgG antibodies, 128 (50%) were positive with Ir 105 antigen, 107 (41.7%) with K 24 and 74 (28.9%) with B 31. The seroprevalence between strains B 31 and K 24 and B 31 and Ir 105 differed statistically significantly (test χ2, p < 0.05), however, the difference between strains K 24 and Ir 105 was insignificant. A significantly higher seroprevalence of all the strains examined was detected in hunting dogs (test χ2, p < 0.05) when compared with service and pet dogs. The seroprevalence correlated with the frequency of outing the dogs in woody areas with the occurrence of borreliae in ticks (R = 0.5 or 0.7) as well as with the frequency of finding engorged ticks (R = 0.5). An epizootiological anamnesis showed a fair specificity in all the strains examined. The Slovak strains showed the higher consistency of positive and negative findings (70%) but statistically lower specificity than the American strain.

Address for correspondence: Astéria Štefančíková, MVD, PhD, Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovak Republic. E-mail: astefan@saske.sk

Key words: Borrelia burgdorferi sensu stricto, Borrelia garini, ticks, dogs, seroprevalence, ELISA, sensitivity, specificity, epidemiology, borreliosis, West Carpathians, Slovak Republic.