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Ann Agric Environ Med 1998, 5, 17-20

ANALYSIS OF THE FREE AMINO ACID CONTENT IN POLLEN OF NINE
ASTERACEAE SPECIES OF KNOWN ALLERGENIC ACTIVITY

Amal K. Mondal, Sanjukta Parui, Sudhendu Mandal

Department of Botany, Visva-Bharati University, Santiniketan, West Bengal, India

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Mondal AK, Parui S, Mandal S: Analysis of the free amino acid content in pollen of nine Asteraceae species of known allergenic activity. Ann Agric Environ Med 1998, 5, 17-20.

Abstract: The study reports the free amino acid composition of the pollen of nine members of the family Asteraceae, i.e. Ageratum conyzoides L., Blumea oxyodonta DC., Eupatorium odoratum L., Gnaphalium indicum L., Mikania scandens Willd., Parthenium hysterophorus L., Spilanthes acmella Murr., Vernonia cinerea (L.) Lees. and Xanthium strumarium L. by thin layer chromatography. The amino acid content was found to vary from 0.5-4.0% of the total dry weight. Fourteen amino acids were identified, among which amino-n-butyric acid, aspartic acid and proline were present in almost all pollen samples. The other major amino acids present in free form included arginine, cystine, glutamic acid, glycine, isoleucine, leucine, methionine, ornithine, tryptophan and tyrosine.

Address for correspondence: Professor Dr Sudhendu Mandal, Head, Department of Botany, Visva-Bharati University, Santiniketan - 731235, West Bengal, India. E-mail: root@vbharat.ernet.in

Key words: free amino acids, pollen, thin layer chromatography, Asteraceae.