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Ann Agric Environ Med 2006, 13, 307-311

CORRELATION OF TESTS FOR DETECTION OF BORRELIA BURGDORFERI
SENSU LATO INFECTION IN PATIENTS WITH DIAGNOSED BORRELIOSIS

Jolanta Chmielewska-Badora1, Ewa Cisak1, Angelina Wojcik-Fatla1, Jacek Zwolinski1,
Alicja Buczek2, Jacek Dutkiewicz1

1Department of Occupational Biohazards, Institute of Agricultural Medicine, Lublin, Poland
2Chair and Department of Biology and Parasitology, Medical University of Lublin, Lublin, Poland

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Chmielewska-Badora J, Cisak E, Wojcik-Fatla A, Zwolinski J, Buczek A, Dutkiewicz J: Correlation of tests for detection of Borrelia burgdorferi sensu lato infection in patients with diagnosed borreliosis. Ann Agric Environ Med 2006, 13, 307-311.

Abstract: A group of 180 patients with diagnosed Lyme borreliosis were examined for the presence of infection with Borrelia burgdorferi sensu lato (B. burgdorferi s.l.) by serologic tests with B. burgdorferi s.l. antigens (IgM-ELISA, IgG-ELISA, IgM-immunoblot, IgG-immunoblot) and by polymerase chain reaction (PCR, nested-PCR) for detection of B. burgdorferi s.l. DNA in peripheral blood. A total of 61.7%, 53.9%, 62.2%, and 59.4% of the examined patientsí sera showed positive or borderline results in the serologic tests IgM-ELISA, IgG-ELISA, IgM-immunoblot, and IgG immunoblot, respectively. The results of the tests IgM-ELISA and IgM-immunoblot were significantly correlated (p<0.001). A higher degree of the correlation (p<0.000001) was found at the comparison of results obtained with IgG-ELISA and IgG-immunoblot. The correlation between the positive findings in the IgM-ELISA and detection with IgM-immunoblot the diagnostically important B. burgdorferi s.l. OspC surface protein was relatively low but statistically significant (0.01B. burgdorferi s.l. antigen, the VlsE protein (p<0.000001). The presence of B. burgdorferi s.l. DNA was found by PCR in 20 out 180 examined blood samples (11.1%). No correlation was found to exist between the PCR results and the results of any of the serologic tests for detection of anti B. burgdorferi s.l. antibodies of IgM class. PCR results correlated significantly at a relatively low level (0.01Borrelia burgdorferi sensu lato.

Address for correspondence: Dr Jolanta Chmielewska-Badora, Department of Occupational Biohazards, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland. E-mail: jcb@galen.imw.lublin.pl

Key words: Lyme borreliosis, Borrelia burgdorferi sensu lato, OspC protein, VlsE protein, serological tests, ELISA, immunoblot, IgG, IgM, PCR, correlation.


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