ORIGINAL ARTICLES

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Ann Agric Environ Med 2006, 13, 301-306

PREVALENCE OF BORRELIA BURGDORFERI GENOSPECIES IN IXODES RICINUS
TICKS FROM LUBLIN REGION (EASTERN POLAND)

Ewa Cisak1, Angelina Wojcik-Fatla1, Nimfa Maria Stojek1, Jolanta Chmielewska-Badora1,
Jacek Zwolinski1, Alicja Buczek2, Jacek Dutkiewicz1

1Department of Occupational Biohazards, Institute of Agricultural Medicine, Lublin, Poland
2Chair and Department of Biology and Parasitology, Medical University of Lublin, Lublin, Poland

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Cisak E, Wojcik-Fatla A, Stojek NM, Chmielewska-Badora J, Zwolinski J, Buczek A, Dutkiewicz J: Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from Lublin region (eastern Poland). Ann Agric Environ Med 2006, 13, 301-306.

Abstract: The objective of the study was to determine the prevalence rate of 3 Borrelia burgdorferi genospecies in Ixodes ricinus ticks collected from wooded areas of the Lublin region (eastern Poland). A group of 1,813 I. ricinus ticks from 6 districts were examined for the presence of Borrelia burgdorferi sensu lato (B.b. s.l.) by polymerase chain reaction (PCR). Another group of 438 I. ricinus ticks collected from 4 districts were examined for the presence of B.b. s.l. by culture on BSKH liquid medium confirmed by PCR, and for the presence of Borrelia spp. by dark field microscopy (DFM). Borrelia burgdorferi genospecies (Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii) were determined by nested-PCR in 113 ticks lysates showing presence of B.b. s.l. (in PCR or in culture and PCR). 5.4% of I. ricinus ticks examined by PCR showed the presence of B.b. s.l. DNA. The infection rate was highest in females (12.1%), lower in males (6.0%) and the lowest in nymphs (1.7%) (p<0.001). The minimum infection rate of I. ricinus ticks with B.b. s.l. determined by culture was 3.4%, whereas the minimum infection rate of ticks with motile spirochetes morphologically resembling Borrelia spp., determined by DFM, amounted to 11.2%. The presence of all 3 Borrelia burgdorferi genospecies under investigation was found in ticks collected from 5 out of 6 examined districts. In 81.4% of infected ticks only single infection with 1 genospecies was observed, while coinfections with 2 or 3 genospecies were detected respectively in 16.8% and 1.8% of infected ticks. Borrelia burgdorferi sensu stricto was the dominant genospecies in all examined tick stages and districts, both in single infections and in coinfections, and found in a total of 62.8% of I. ricinus ticks infected with B.b. s.l. Borrelia afzelii and Borrelia garinii were less frequent and observed in respectively 39.8% and 17.8% of infected ticks.

Address for correspondence: Dr Ewa Cisak, Department of Occupational Biohazards, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland. E-mail: ewac@galen.imw.lublin.pl

Key words: ticks, Ixodes ricinus, Borrelia burgdorferi sensu lato, Borrelia burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii, PCR, culture, dark field microscopy, eastern Poland.


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