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Ann Agric Environ Med 2005, 12, 321-323 | |
APPLICATION OF SPECIFIC PRIMERS IN THE DIAGNOSIS OF
ENCEPHALITOZOON SPP.
Alexandra Valencakova1, Pavol Balent1, Frantisek Novotny2,
Lydia Cislakova3
1Department of Biology, University of Veterinary Medicine, Kosice, Slovak Republic
2Department of Gynecology, University of Veterinary Medicine, Kosice, Slovak Republic
3Department of Epidemiology, Faculty of Medicine, P. J. Safarik University, Kosice, Slovak Republic
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Valencakova A, Balent P, Novotny F, Cislakova L: Application of specific primers in the diagnosis of Encephalitozoon spp.. Ann Agric Environ Med 2005, 12, 321-323. |
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Abstract: In our experiment, 3 species-specific primer pairs cultivated in cell lines were used: Encephalitozoon cuniculi-specific primer pairs (ECUNF and ECUNR), Encephalitozoon hellem-specific primer pairs (EHELF and EHELR), and Encephalitozoon intestinalis-specific primer pairs (SINTF and SINTR). The PCR products were estimated to be 550 bp in E. cuniculi, 547 bp in E. hellem and 545 bp in E. intestinalis respectively, which can prove the precision and reliability of this method in the species identification of the genus Encephalitozoon. All 3 primer pairs were species-specific and none of them amplified gene sequences from other Encephalitozoon spp. |
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Address for correspondence: Dr. Alexandra Valencakova, PhD., Department of Biology, University of Veterinary Medicine, Komenskeho 73, 041 81, Kosice, Slovak Republic. E-mail: valencakova@uvm.sk |
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Key words: Encephalitozoon cuniculi, Encephalitozoon intestinalis, Encephalitozoon hellem, PCR, specific primers. |