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Ann Agric Environ Med 2004, 11, 351-354

DETECTING DNAS OF ANAPLASMA PHAGOCYTOPHILUM AND BABESIA
IN THE BLOOD OF PATIENTS SUSPECTED OF LYME DISEASE

Teresa Hermanowska-Szpakowicz1, Bogumila Skotarczak2, Maciej Kondrusik1,
Anna Rymaszewska2, Marek Sawczuk2, Agnieszka Maciejewska2,
Malgorzata Adamska2, Slawomir Pancewicz1, Joanna Zajkowska1

1Department of Infectious Diseases and Neuroinfections, Medical University of Bialystok, Poland
2Division of Genetics of Szczecin University, Szczecin, Poland


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Hermanowska-Szpakowicz T, Skotarczak B, Kondrusik M, Rymaszewska A, Sawczuk M, Maciejewska A, Adamska M, Pancewicz S, Zajkowska J: Detecting DNAs of Anaplasma phagocytophilum and Babesia in the blood of patients suspected of Lyme disease. Ann Agric Environ Med 2004, 11, 351-354.

Abstract: Co-occurrence of granulocytic anaplasmosis, borreliosis and babesiosis in humans is a result of common vectors for the respective pathogens of these diseases, most commonly ticks from the genus Ixodes. Studies on ticks in Europe and also in Poland have shown that several pathogens may co-occur in individuals of I. ricnus. A total of 96 hospitalised patients infected or suspected of being infected with borreliosis were screened for A. phagocytophilum and Babesia sp. DNA. Positive results of PCRs for A. phagocytophilum DNA were obtained for 10 patients, 8 of whom were diagnosed with borreliosis earlier, and 4 of whom were diagnosed with tick-borne encephalitis (on the basis of serological studies of serum and cerebrospinal fluid). None of the 10 patients had clinical or biochemical markers of anaplasmosis, corroborating the existence of asymptomatic anaplasmosis or self-limiting course. in Europe. Similarly, Babesia DNA was not found in the blood of any of the patients. The results of the studies show that in diagnosing tick-borne diseases, clinical examinations should consider infection by two or even three tick-borne pathogens.

Address for correspondence: B. Skotarczak, Division of Genetics of Szczecin University, Szczecin, Piastow 40B.
E-mail: Bogumila_Skotarczak@sus.univ.szczecin.pl

Key words: Anaplasma phagocytophilum, Babesia, PCR, patients suspected of Lyme disease.


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