RESEARCH PAPER
The viability and intestinal epithelial cell adhesion of probiotic strain combination - in vitro study
 
More details
Hide details
1
Department of Allergology and Environmental Hazards, Institute of Rural Health, Lublin, Poland
 
2
Department of Physiology, University of Medical Sciences, Poznan, Poland
 
3
Department of Biotechnology and Microbiology of Food, University of Life Sciences, Poznan, Poland
 
4
Department of Pediatric Orthopaedics, University of Medical Sciences, Poznan, Poland
 
 
Corresponding author
Jacek Piątek   

Department of Physiology, University of Medical Sciences, Poznan, Poland
 
 
Ann Agric Environ Med. 2012;19(1):99-102
 
KEYWORDS
ABSTRACT
To be effective, probiotic bacteria must exhibit a number of functional characteristics, including the resistance to gastric acidity and the ability to adhere to the intestinal epithelium. In this study, we examined in vitro the viability of lactic acid bacteria (LAB) combination after exposure to low pH, and the adhesion of LAB to Caco-2 cells during coincubation of 9 bacterial strains. To test bacterial viability, 6 commercially available products were incubated in 0.1 N HCl at pH 1.2 for 60 min. The greatest growth inhibition was noted for the non-capsulated product containing the Lactobacillus rhamnosus strain (log reduction of CFU = 6.4), and the best survival observed for the product containing 9 bacterial strains, equipped with a modern capsule made according to the Multi-Resistant Encapsulation technology (log reduction of CFU = 0.1). In the adhesion experiment, the combination of 9 bacterial strains was added to 17-day-old Caco-2 cell culture for 90 min. The greatest efficiency of adhesion was observed for the inoculum containing 5.5x108CFU/mL/9.6 cm2 of Caco-2 and the dose of probiotic bacteria of 190 cells per one Caco-2 cell. As a result, approximately 157 bacterial cells adhered to one Caco-2 cell. The results indicate that the combination of 9 bacterial strains in the examined product is characterized as highly adhesive.
 
REFERENCES (27)
1.
Fuller R. Probiotics in man and animals: J Appl Bacteriol. 1989; 66: 365-378.
 
2.
Nighswogner BD, Brashears MM, Gilliand SE. Viability of Lactobacillus acidophillus and lactobacillus casei in fermented milk products during refrigerated storage. J Dairy Sci. 1996; 79: 212-219.
 
3.
Corneliussen L, Skov R, Espersen F. Effect of low pH on survival of lactic producing bacteria in oral formulation. In: Clin Microbiol Inf Pharmacokinetics/Pharmacodynamics 2008; P 785. The 9 th European Congress of Clinical Microbiology and infectious Diseases, Berlin, Germany 1999.
 
4.
Kim KI, Baek YJ, Yoon YH. Effects of rehydration media and immobilisation in calcium-alginate on the survival of Lactobacillus casei and Bifidobacterium bifidum. Korean J Dairy Sci. 1996; 18: 193-198.
 
5.
Salminen S, Isolauri E, Salminen E. Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges. Antonie Van Leeuwenhoek 1996; 70: 251-62.
 
6.
Guarner F, Schaafsma GJ. Probiotics. Int J Food Microbiol. 1998; 39: 237-8.
 
7.
Candela M, Seibold G, Vitali B, Lachenmaier S, Eikmanns BJ, Brigidi P. Real-time PCR quantification of bacterial adhesion to Caco-2 cells: competition between bifidobacteria and enteropathogens. Res Microbiol. 2005; 156: 887-895.
 
8.
Isolauri E, Joensuu J, Suomalainem H. Improved immunogenicity of oral Dx RRV reassortant rotavirus vaccine by Lactobacillus casei GG. Vaccine. 1995; 13: 310-312.
 
9.
Guarino A, Canani RB, Spagnuolo MI, Albano F, di Benedetto L. Oral bacterial therapy reduces the duration of symptoms and viral excretion in children with mild diarrhoea. J Pediatr Gastroenterol Nutr. 1998; 25: 516-519.
 
10.
Marteau PR, de Vrese M, Cellier CJ, Schrezenmeir J. Protection from gastrointestinal diseases with the use of probiotics. Am J Clin Nutr. 2001; 73 (2 Suppl): 430S-436S.
 
11.
Sarem-Damerdjii LO, Sarem F, Marchal L. In vitro colonization ability of human colon mucosa by exogenous Lactobacllus strains. FESM Microbiol Lett. 1995; 131: 133-137.
 
12.
Huis In’t Veld JHJ, Shortt C. Selection criteria for probiotic microorganisms. London: The Royal Society of Medicine Press Ltd, International Congress and Symposium Series, 1996, 219, 27-36.
 
13.
Bertazzoni Minelli E, Benini A, Marzotto M, Sbarbati A, Ruzzenente O, Ferrario R, Hendrinks H, Dellaglio F. Assessment of novel probiotic Lactobacillus casei strains for the production of functional dairy foods. Int Dairy J. 2004; 14: 723-736.
 
14.
Winding, A., S.J. Binnerup and J. Sorensen. 1994. Viability of indigenous soil bacteria assayed by respiratory activity and growth. Appl Environ Microbiol. 60: 2869-2875.
 
15.
Dunne C, O’Mahony L, Murphy L, Thornton G, Morrissey D, O’Halloran S, Feeney M, Flynn S, Fitzgerald G, Daly C, Kiely B, O’Sullivan G.C, Shanahan F, Collins JK. In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am J Clin Nutri. 2001; 73(2): 386-392.
 
16.
Ding WK, Shah NP. Acid, Bile, and Heat Tolerance of Free and Microencapsulated Probiotic Bacteria. J Food Sci. 2007; 72(9): M446-450.
 
17.
Sheu TY, Marshall RT, Heymann H. Improving survival of culture bacteria in frozen desserts by microentrapment. J Dairy Sci. 1993; 76: 1902-1907.
 
18.
Rao AV, Shiwnarain N, Maharaj I. Survival of microencapsulated Bifidobacterium pseudolongum in simulated gastric and intestinal juices. Can Inst Food Sci Technol J. 1989; 22: 345-349.
 
19.
Khater KAA, Ali MA, Ahmed EAM. Effect of Encapsulation on some Probiotic Criteria. J Am Sci. 2010; 6(10): 836-845.
 
20.
Tuomola EM, Salminen SJ. Adhesion of some probiotic and dairy Lactobacillus strains to Caco-2 cell cultures. Int J Food Microbiol. 1998; 41: 45-51.
 
21.
Moussavi M, Adams MC. An In Vitro Study on Bacterial Growth Interactions and Intestinal Epithelial Cell Adhesion Characteristics of Probiotic Combinations. Curr Microbiol. 2010; 60: 327-335.
 
22.
Gopal PK, Prasad J, Smart J, Gill HS. In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli. Int J Food Microbiol. 2001; 67: 207-216.
 
23.
Candela M, Perna F, Carnevali P, Vitali B, Ciati R, Gionchetti P, Rizzello F, Campieri M, Brigidi P. Interaction of probiotic Lactobacillus and Bifidobacterium strains with human intestinal epithelial cells: Adhesion properties, competition against enteropathogens and modulation of IL-8 production. Int J Food Microbiol. 2008; 125: 286-292.
 
24.
Gagnon M, Kheadr EE, Blay G, Fliss I. In vitro inhibition of Escherichia coli O157:H7 by bifidobacterial strains of human origin. Int J Food Microbiol. 2003; 92: 69-78.
 
25.
Ouwehand AC, Kirjavainen PV, GroKnlund MM, Isolauri E, Salminen SJ. Adhesion of probiotic micro-organisms to intestinal mucus. Int Dairy J. 1999; 623-630.
 
26.
Collado MC, Jalonen L, Meriluoto J, Salminen S. Protection mechanism of probiotic combination against human pathogens: in vitro adhesion to human intestinal mucus. Asia Pac J Clin Nutr. 2006; 15(4): 570-5.
 
27.
Collado MC, Meriluoto J, Salminen S. In vitro analysis of probiotic strain combinations to inhibit pathogen adhesion to human intestinal mucus. Food Res Int. 2007; 40: 629-636.
 
eISSN:1898-2263
ISSN:1232-1966
Journals System - logo
Scroll to top