TY - JOUR JO - Annals of Agricultural and Environmental Medicine J2 - Ann Agric Environ Med. SN - 1232-1966 VL - 24 IS - 1 PY - 2017 ID - Sikora2017 TI - Assessment of antibiotic susceptibility of Legionella pneumophila isolated from water systems in Poland AB - Introduction and objective Several studies have reported therapy failures in patients with legionnaires’disease; however, antimicrobial resistance of clinical and environmental isolates of Legionella spp. has not yet been documented. Routine susceptibility testing of Legionella spp. is not recommended because of difficulties in determining standard minimal inhibitory concentration values. The purpose of this study was to analyze the antimicrobial susceptibility of Legionella pneumophila. strains isolated from a water supply system. Material and Methods Twenty-eight isolates of L. pneumophila (16 – L. pneumophila SG 1, 12 – L. pneumophila SG 2–14) obtained from water systems in public buildings in Poland were tested. Susceptibility testing was performed using the E-test method. The tested antibiotic were azithromycin, ciprofloxacin, and rifampicin. The medium used for the susceptibility testing was BCYE-, a special medium for Legionella cultivation. Results Among the tested strains, L. pneumophila was the only one resistant to azithromycin. It was a strain of L. pneumophila SG 2–14 isolated from the water system in a sanitorium. All isolates were found to be sensitive to ciprofloxacin and rifampicin. However, the azithromycin-resistant strain exhibited higher ciprofloxacin and rifampicin MIC (1.5 μg/ml, and 0.19 μg/ml, respectively). The MIC50 for azithromycin, ciprofloxacin, and rifampicin were 0,032, 0,125, and 0,003 μg/ml, respectively. The MIC90 for azithromycin, ciprofloxacin, and rifampicin were 0,032, 0,125, and 0,003 μg/ml, respectively. Conclusions Azithromycin resistance was found in one strain of L. pneumophila SG 2–14, but the resistance mechanism is unknown and needs further study. It is possible that therapeutic failures in Legionnaires’ disease may be associated with bacterial resistance which should be taken into account. The antibiotic sensitivity testing described in this study could be helpful in detecting the resistance of clinical L. pneumophila isolates. Ciprofloxacin and rifampicin have good in vitro activity against environmental L. pneumophila SG 1 and SG 2–14 in Poland. AU - Sikora, Agnieszka AU - Gładysz, Iwona AU - Kozioł-Montewka, Maria AU - Wójtowicz-Bobin, Małgorzata AU - Stańczak, Tomasz AU - Matuszewska, Renata AU - Krogulska, Bożena SP - 66 EP - 69 DA - 2017 DO - 10.5604/12321966.1234048 UR - https://doi.org/10.5604/12321966.1234048 ER -