TY - JOUR JO - Annals of Agricultural and Environmental Medicine J2 - Ann Agric Environ Med. SN - 1232-1966 VL - 23 IS - 1 PY - 2016 ID - Bártová2016 TI - Neospora caninum and Toxoplasma gondii antibodies in red foxes (Vulpes vulpes) in the Czech Republic AB - Introduction Neospora caninum and Toxoplasma gondii are worldwide spread parasites, causing serious illnesses in sensitive animals; toxoplasmosis is also important zoonosis. Although neosporosis is not considered as a zoonosis, it leads to aborted births in cattle, as well as paresis and paralysis in dogs. Objective The aim of this study was to discover the prevalence of N. caninum and T. gondii antibodies in red foxes ( Vulpes vulpes ) in the Czech Republic. Material and Methods Sera of 80 foxes from 8 regions of the Czech Republic were tested for antibodies to N. caninum and T. gondii by competitive enzyme linked immunosorbent assay (cELISA) and indirect ELISA. All samples were simultaneously tested by indirect fluorescent antibody test (IFAT) to detect both N. caninum and T. gondii antibodies. Results Antibodies to N. caninum were found by IFAT in 3 (3.8%) red foxes with titre 50 and in 2 (2.5%) red foxes with inhibition 42.7% and 30.2 %. Antibodies to T. gondii were found in all tested animals in both IFAT (titres 50 – 6400) and in ELISA (S/P ranging from 34% – 133%). Conclusions This is the first prevalence study of N. caninum and T. gondii antibodies in red foxes in the Czech Republic. The results obtained show that red foxes are exposed at different levels to both protozoan infections, and thus could play an important role in the transmission cycle of N. caninum and T. gondii in sylvatic cycle. AU - Bártová, Eva AU - Slezáková, Radka AU - Nágl, Ivan AU - Sedlák, Kamil SP - 84 EP - 86 DA - 2016 DO - 10.5604/12321966.1196858 UR - https://doi.org/10.5604/12321966.1196858 ER -