TY - JOUR JO - Annals of Agricultural and Environmental Medicine J2 - Ann Agric Environ Med. SN - 1232-1966 VL - 22 IS - 3 PY - 2015 ID - Łuczaj2015 TI - Peroxidative metabolism of arachidonic acid in the course of Lyme arthritis AB - Objective The objective of the study was measurement of serum arachidonic acid level as well as the product of its peroxidation – 8-isoPGF 2α , and the activity of phospholipase A 2 and PAF-acetylhydrolase that participate in releasing 8-isoPGF 2α from glycerol skeleton, and the potential designation of their role in the pathomechanism of Lyme disease (LD). Material and Methods Changes in the phospholipid arachidonic acid level and the level of 8-isoPGF 2α were determined in the plasma and urine of patients with LA (n=57) and of healthy controls (n=41). The activity of phospholipase A 2 and PAF acetylhydrolase were assayed. All examined parameters were also measured in the plasma of some LA patients (n=13) after antibiotics treatment. Results An almost 3-fold higher level of the total plasma 8-isoPGF 2α was observed in LA patients compared to the controls, while in the urine it increased over 5-fold. The plasma PLA 2 activity was more than 3-fold higher in LA patients than in the healthy subjects, while PAF acetylhydrolase activity was observed to be modestly, but not significantly lower. The total 8-isoPGF 2α level in the plasma and urine of LA patients was significantly lower after antibiotics treatment. The plasma activity of PAF-AH in the LA patients was increased, while the cPLA 2 activity decreased after antibiotics treatment. Conclusions It may be suggested that in the course of LA, the level of binding 8-isoPGF 2α is significantly enhanced, and it may also be suggested that uncontrolled changes in the lipid status of some patients may make their Lyme arthritis unresponsive to antibiotics. AU - Łuczaj, Wojciech AU - Moniuszko, Anna AU - Rusak, Magdalena AU - Zajkowska, Joanna AU - Pancewicz, Sławomir AU - Skrzydlewska, Elżbieta SP - 433 EP - 437 DA - 2015 DO - 10.5604/12321966.1167708 UR - https://doi.org/10.5604/12321966.1167708 ER -