TY - JOUR JO - Annals of Agricultural and Environmental Medicine J2 - Ann Agric Environ Med. SN - 1232-1966 VL - 22 IS - 2 PY - 2015 ID - Tanveer2015 TI - Quick survey for detection, identification and characterization of Acanthamoeba genotypes from some selected soil and water samples in Pakistan AB - Acanthamoeba is an opportunistic protozoan pathogen which is widely distributed in nature and plays a pivotal role in ecosystem. Acanthamoeba species may cause blinding keratitis and fatal granulomatous encephalitis involving central nervous system. In this study, we investigated the presence of Acanthamoeba in soil and water resources of Pakistan. Here, Acanthamoeba were recovered on non-nutrient agar plate lawn with E.coli and identified by morphological characteristics of the cyst. Furthermore PCR was performed with genus-specific primers followed by direct sequencing of the PCR product for molecular identification. Overall our PCR and sequencing results confirmed pathogenic genotypes including T4 and T15 from both soil and water samples. This is our first report of Acanthamoeba isolation from both soil and water resources of Pakistan which may serve as a potential treat to human health across the country. AU - Tanveer, Tania AU - Hameed, Abdul AU - Gul, Asma AU - Matin, Abdul SP - 227 EP - 230 DA - 2015 DO - 10.5604/12321966.1152070 UR - https://doi.org/10.5604/12321966.1152070 ER -