TY - JOUR JO - Annals of Agricultural and Environmental Medicine J2 - Ann Agric Environ Med. SN - 1232-1966 VL - 16 IS - 1 PY - 2009 ID - Czarnobilska2009 TI - Contact allergy to nickel: patch test score correlates with IL-5, but not with IFN-gamma nickel-specific secretion by peripheral blood lymphocytes. AB - Traditionally, allergic contact dermatitis (ACD) has been associated with the activity of Th1 lymphocytes that secrete interferon gamma. Recent evidence indicates that other cells, e.g. interleukin 5 (IL-5)-secreting Th2 or Tc2 cells may be among the key effectors of ACD. The aim of the present study was to assess the influence of nickel-specific IFN-gamma secretion (marker of Th1 and Tc1 activity) and IL-5 secretion (Th2 and Tc2) on the clinical outcome (patch test score) in nickel-allergic patients. 40 women with suspicion of ACD were involved, aged from 14-54 (median 31.5) years. They were patch tested with NiSO 4 . Peripheral blood mononuclear cells (PBMC) from the patients were cultured and analysed for IFN-gamma and IL-5 secretion in response to NiSO 4 . A series of statistical models (classical logit or cloglog link function) were used. We demonstrate that nickel-specific IL-5 secretion by PBMC is correlated with the intensity of patch test reaction (p=0.05), with no significant effect of IFN-gamma. An increase in the nickel-specific IL-5 secretion from PBMC by 10 pg/ml is associated with a 10-20% increase (depending on statistical model) in the odds ratio of the patient to have a higher patch test score. These findings support the assumption that cells secreting IL-5 (e.g. Th2, Tc2) play a more important role in the pathogenesis of ACD than previously thought. AU - Czarnobilska, Ewa AU - Jenner, Bartosz AU - Kaszuba-Zwoinska, Jolanta AU - Kapusta, Maria AU - Obtułowicz, Krystyna AU - Thor, Piotr AU - Śpiewak, Radosław SP - 37 EP - 41 DA - 2009 UR - https://www.aaem.pl/Contact-allergy-to-nickel-patch-test-score-correlates-with-IL-5-but-not-with-IFN-gamma-nickel-specific-secretion-by-peripheral-blood-lymphocytes-,71589,0,2.html ER -