%0 Journal Article %9 journal article %J Annals of Agricultural and Environmental Medicine %@ 1232-1966 %V 23 %N 1 %D 2016 %F Raszewski2016 %T Cytotoxicity induced by cypermethrin in Human Neuroblastoma Cell Line SH-SY5Y %X The purpose of this study was to evaluate the cytotoxic potential of Cypermethrin (CM) on cultured human Neuroblastoma SH-SY5Y cells. SH-SY5Y cells were treated with CM at 0–200µM for 24, 48, and 72 h, in vitro . It was found that CM induced the cell death of Neuroblastoma cells in a dose- and time-dependent manner, as shown by LDH assays. Next, some aspects of the process of cell death triggered by CM in the human SH-SY5Y cell line were investigated. It was revealed that the pan-caspase inhibitor Q-VD-OPh, sensitizes SH-SY5Y cells to necroptosis caused by CM. Furthermore, signal transduction inhibitors PD98059, SL-327, SB202190, SP600125 failed to attenuate the effect of the pesticide. Finally, it was shown that inhibition of TNF-a by Pomalidomide (PLD) caused statistically significant reduction in CM-induced cytotoxicity. Overall, the data obtained suggest that CM induces neurotoxicity in SH-SY5Y cells by necroptosis. %A Raszewski, Grzegorz %A Lemieszek, Marta Kinga %A Łukawski, Krzysztof %P 106-110 %R 10.5604/12321966.1196863 %U https://doi.org/10.5604/12321966.1196863