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The influence of protein deficient diet, vitamin B2 supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats.

Aneta Lewicka 1,  
Anna Klos 1,  
Bogdan Debski 3,  
Tomasz Kurył 3,  
Department of Hygiene and Physiology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland
Department of Regenerative Medicine, Military Institute of Hygiene and Epidemiology, Warsaw, Poland
Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Poland
Abstract. Introduction and objective: Prolonged shortages of protein in the diet significantly alter composition and content of polyunsaturated fatty acids (PUFA) in tissues and body fluids. One of nutritional factors which may reduce negative effects of protein malnutrition might be vitamin B2 due to influence on lipids metabolism. The aim of the study was to investigate the influence of protein-deficient diet enriched with vitamin B2 on content and composition of PUFA in the blood serum of rats treated with dosed physical exercise. Materials and methods: The experiment carried out for 3 months and was performed on 72 growing male Wistar rats divided into 5 groups. Animals were fed ad libitum with diet of energy value of 350 kcal/100 g in which 4.5% of energy was provided by protein. In control diet 20% of energy was provided by protein. Two groups were fed the diet enriched with vitamin B2. Two groups of tested animals were trained for 5 days a week. Results: Protein-deficient diet caused decrease of α-linolenic acid (ALA) after 30 days and decrease of docosahexaenoic acid (DHA) after 60 days of experiment compared to rats fed control diet. After 60 and 90 days of experiment significant decrease of arachidonic acid (AA) in serum of trained rats fed a protein diet compared sedentary rats fed the same diet. Physical exercise of animals fed 90 days low protein (LP) diet caused decrease of AA despite of vitamin B2 enrichment. Conclusions: Results of this study suggest that all investigated factors (protein deficiency, physical exercise and supplementation of vitamin B2) have significant impact on PUFA composition of serum in rats.