A case of bovine tuberculosis in pigs in Poland – a country free from the disease
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National Veterinary Research Institute, Pulawy, Poland
Bovine tuberculosis is a chronic contagious disease caused by Mycobacterium bovis or Mycobacterium caprae. Before widespread action conducted in Poland between 1959–1975 to combat bovine tuberculosis (BTB), about 40% of all tuberculosis cases in pigs was caused by the bovine bacillus. At the present time, correctly carried out, long-term control of cattle has resulted in cases of bovine tuberculosis in pigs and humans being extremely rare and sporadic. In pigs, tuberculosis is most often caught in a slaughterhouse during slaughter.

Material and methods:
Samples came from pigs kept on the farm. Traditional bacteriological methods on solid media (Stonebrink, LJ with pyruvate) supported by the semi-automatic, liquid indicative culture method (MGIT) and PCR test were applied in targeted studies. The GenoType Mycobacterium MTBC and CM tests (Hain Lifescience, Germany) were used to additionally confirm that isolated strains classification was used.

Strains of mycobacteria were isolated from all examined pigs. Mycobacterium bovis was determined by real time PCR and Hain Genotype methods.

In order to effectively fight against BTB, all animals on farms should be tested, regardless of species, while the milk of suspected cows should be utilized without being used for feed. It is important to adapt the current legal regulations to the current epidemiological situation.

Marek Lipiec   
National Veterinary Research Institute, Pulawy, Poland, 24-100 Puławy, Partyzantów 57, Puławy, Poland, 24-100 Puławy, Poland
1. Sobiech T. Gruźlica zwierząt. Państwowe Wydawnictwo Rolnicze i Leśne, Warszawa, 1975.
2. Rozporządzenie Ministra Rolnictwa i Rozwoju Wsi z dnia 23 listopada 2004 r. w sprawie zwalczania gruźlicy bydła (Dz. U. z dnia 6 grudnia 2004 r.).
3. Instrukcja Głównego Lekarza Weterynarii Nr GIWpr-022010–8/2016, z dnia 8 lutego 2016 r. w sprawie postępowania przy podejrzeniu, potwierdzeniu i zwalczaniu w stadzie bydła oraz przy prowadzeniu badań kontrolnych gruźlicy bydła.
4. LoBue PA, Enarson DA, Thoen CO. Tuberculosis in humans and animals: an overview. Int Tuberc Lung Dis. 2010; 14(9): 1075–1078.
5. Krajewska M, Lipiec M, Zabost A, Augustynowicz-Kopeć E, Szulowski K. Bovine tuberculosis in a wild boar (Sus scrofa) in Poland. J Wildl Dis. 2014; 50(4): 1001–1002. doi: 10.7589/2013-07-187.
6. Krajewska M, Zabost A, Welz M, Lipiec M, Orłowska B, Anusz K, Brewczyński P, Augustynowicz-Kopeć E, Szulowski K, Bielecki W, Weiner M. Transmission of Mycobacterium caprae in a herd of European bison in the Bieszczady Mountains, Southern Poland. Eur J Wildlife Res. 2015; 61(3): 429–433.
7. Javed MT, Aranaz A, de Juan L, Bezos J, Romero B, Alvarez J, Lozano C, Mateos A, Domínguez L. Improvement of spoligotyping with additional spacer sequences for characterization of Mycobacterium bovis and M. caprae isolates from Spain. Tuberculosis (Edinb). 2007; 87(5): 437–445.
8. Reddington K, O’Grady J, Dorai-Raj S, Niemann SA. Novel Multiplex Real-Time PCR for the identification of mycobacteria associated with zoonotic tuberculosis. PLoS ONE. 2011; 6(8): 1–8.
9. Harris KA, Brunton L, Brouwer A, Garcia MP, Gibbens JC, Smith NH, Upton PA. Bovine TB infection status in cattle in Great Britain in 2015. Vet Rec. 2017; 180(7): 170–175.
10. Broughan JM, Downs SH, Crawshaw TR, Upton PA, Brewer J, Clifton-Hadley RS. Mycobacterium bovis infections in domesticated non-bovine mammalian species. Part 1: Review of epidemiology and laboratory submissions in Great Britain 2004–2010. Vet J. 2013; 198(2): 339–345.
11. Bailey SS, Crawshaw TR, Smith N, Palgrave CJ. Mycobacterium bovis infection in domestic pigs in Great Britain. Vet J. 2013; 198(2): 346–351.
12. Di Marco V, Mazzone P, Capucchio MT, Boniotti MB, Aronica V, Russo M, Fiasconaro M, Cifani N, Corneli S, Biasibetti E, Biagetti M, Pacciarini ML, Cagiola M, Pasquali P, Marianellif C. Epidemiological significance of the domestic black pig (Sus scrofa) in maintenance of bovine tuberculosis in Sicily. J Clin Microbiol. 2012; 50(4): 1209–1218.
13. Cardoso-Toset F, Luque I, Carrasco L, Jurado-Martos F, Risalde MÁ, Venteo Á, Infantes-Lorenzo JA, Bezos J, Rueda P, Tapia I, Gortázar C, Domínguez L, Domínguez M, Gomez-Laguna J. Evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern Spain. Prev Vet Med. 2017; 1(137): 101–104.
14. Barandiaran S, Martınez Vivot M, Moras EV, Cataldi AA. Mycobacterium bovis in swine: spoligotyping of isolates from Argentina. Vety Med Int. 2011; 1–6, doi: 10.4061/2011/979647.
15. Barandiaran S, M. Martínez Vivot M, Pérez AM, Cataldi AA, Zumarraga MJ. Bovine tuberculosis in domestic pigs: Genotyping and distribution of isolates in Argentina. Res Vet Sci. 2015; 44–50, doi: 10.1016/j.rvsc.2015.09.013.
16. Muwonge A, Johansen TB, Vigdis E, Godfroid J, Olea-Popelka F, Biffa D, Skjerve E, Djønne B. Mycobacterium bovis infections in slaughter pigs in Mubende district, Uganda: a public health concern. BMC Vet Res. 2012; 8: 168, doi: 10.1186/1746-6148-8-168.
17. Arega SM, Conraths FJ, Gobena A. Prevalence of tuberculosis in pigs slaughtered at two abattoirs in Ethiopia and molecular characterization of Mycobacterium tuberculosis isolated from tuberculous-like lesions in pigs. BMC Vet Res. 2013; 9: 97, doi: 10.1186/1746-6148-9-97.