Small rodents as reservoirs of Cryptosporidium spp. and Giardia spp. in south-western Poland
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Department of Parasitology, Institute of Genetics and Microbiology, Wroclaw University, Poland
Department of Invertebrate Systematics and Ecology, Institute of Biology, Wroclaw University of Environmental and Life Sciences, Poland
Ann Agric Environ Med. 2015;22(1):1–5
Cryptosporidium spp. and Giardia spp. have been detected in a range of host species, including rodents. The aim of this study was to determine the distribution of these pathogens and recognition of the reservoir role of rodents in the maintenance of these pathogens in south-western Poland. Additionally, preliminary molecular studies were conducted to elucidate the species and genotypes of Cryptosporidium and Giardia identified in this study. Stool samples (n=266) from A. agrarius, A. flavicollis and M. glareolus, were subjected for analyses. Values of prevalence were 61.7, 68.3 and 68.1%, respectively, for Cryptosporidium spp. and 41.7, 24.4 and 38.4%, respectively, for Giardia spp. There was a statistically significant correlation between host species and Giardia infection where A. agrarius was the species of the highest prevalence. Statistically significant differences were not found for comparisons made for study sites and occurrence of Giardia spp. and Cryptosporidium spp. Due to preliminary nested PCR results, specific amplifications of Cryptosporidium COWP and SSU rRNA genes were obtained for several isolates taken from rodent host species. One isolate recovered from A. agrarius (from a semi-aquatic, urban area) was identified as C. parvum and revealed 100% similarity with sequences obtained from humans. To the best of the knowledge of the authors, this is the first record of the C. parvum zoonotic species from the striped field mouse. Also recorded were the first findings of C. ubiquitum from three small rodent species.
Xiao L, Fayer R. Molecular characterization of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission. Int J Parasitol. 2008; 38: 1239–1255.
Pacha RE, Clark GW, Williams EA, Carter AM, Scheffelmaier JJ, Debusschere P. Small rodents and other mammals associated with mountain meadows as reservoirs of Giardia spp. and Campylobacter spp. Appl Environ Microbiol. 1987; 53: 1574–1579.
Chalmers RM, Sturdee AP, Bull SA, Miller A, Wright SE. The prevalence of Cryptosporidium parvum and C. muris in Mus domesticus, Apodemus sylvaticus and Clethrionomys glareolus in an agricultural system. Parasitol Res. 1997; 83: 478–482.
Torres J, Gracenea M, Gomez MS, Arriza-Balaga A, Gonzalez-Moreno A. The occurrence of Cryptosporidium parvum and C. muris in wild rodents and insectivores in Spain. Vet Parasitol. 2000; 92: 253–260.
Hajdusek O, Ditrich O, Slapeta J. Molecular identification of Cryptosporidium spp. in animal and human hosts from the Czech Republic. Vet Parasitol. 2004; 122: 183–192.
Feng Y, Alderisio K, Yang W, Blancero LA, KuhneWG, Nadareski CA, Reid M, Lihua Xiao L. Cryptosporidium genotypes in wildlife from a New York watershed. Appl Environ Microb. 2007; 6475–6483.
Ziegler PE, Wade SE, Schaaf SL, ChangYF, Mohammed HO. Cryptosporidium spp. from small mammals in the New York City watershed. J Wild Dis. 2007; 43: 586–596.
Lv C, Zhang L, Wang R, Jian F, Zhang S, Ning C, Wang H, Feng C, Wang X, Ren X, Qi M, Xiao L. Cryptosporidium spp. in wild, laboratory, and pet rodents in China: prevalence and molecular characterization. Appl Environ Microbiol. 2009; 75: 7692–7699.
Bajer A, Bednarska M, Pawełczyk A, Behnke JM, Gilbert FS, Siński E. Prevalence and abundance of Cryptosporidium parvum and Giardia spp. in wild rural rodents from Mazury Lake District region of Poland. Parasitology 2002; 125: 21–34.
Bajer A. Between-year variation and spatial dynamics of Crypto sporidium spp. and Giardia spp. infections in naturally infected rodent populations. Parasitology 2008; 135: 1629–1649.
Solarczyk P, Majewska AC. Molecular identification of Giardia spp. and Cryptosporidium spp. in humans and animals. Wiad Parazytol. 2007; 53: 110.
Bajer A, Caccio S, Bednarska M, Behnke JM, Pieniazek J, Sinski E. Preliminary molecular characterization of Cryptosporidium parvum isolates of wildlife rodents from Poland. J Parasitol. 2003; 89: 1053–1055.
Cacciò SM, Beck R, Almeida A, Bajer A, Pozio A. Identification of Giardia species and Giardia duodenalis assemblages by sequence analysis of the 5.8S rDNA gene and internal transcribed spacers. Parasitology 2010; 137: 919–925.
Fayer R, Santin M, Macarisin D. Cryptosporidium ubiquitum n. sp. in animals and humans. Vet Parasitol. 2010; 172: 23–32.
Henriksen SA, Pohlenz JFL. Staining of cryptosporidia by a modified Ziehl – Neelsen technique. Acta Vet Scand. 1981; 22: 594–596.
Xiao L, Escalante L, Yang C, Sulaiman I, Escalante AA, Montali RJ, Fayer R, Lal AA. Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl Environ Microb. 1999; 1578–1583.
Pedraza-Dıaz S, Amar C, Nichols GL, McLauchlin J. Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene. Emerg Infect Dis. 2001; 7: 49–56.
Hopkins RM, Meloni BP, Groth DM, Wetherall JD, Reynoldson JA, Thompson RC . Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality. J Parasitol. 1997; 83: 44–51.
Bush AO, Lafferty KD, Lotz JM, Shostak SW. Parasitology meets ecology on its own terms: Margolis etal. revisited. J Parasitol. 1997; 83: 575–583.
Siński E, Bednarska M, Bajer A. The role of wild rodents in ecology of cryptosporidiosis in Poland. Folia Parasit. 1998; 45: 173–174.
Bajer A, Bednarska M, Siński E. Środowiskowe uwarunkowania zarażeń Cryptosporidium parvum w populacjach drobnych gryzoni. Wiad Parazytol. 2001; 47: 747–753 (in Polish).
Majewska AC, Werner A, Słodkowicz A, Piłacińska B. Prevalence of intestinal protozoa parasites in wild rodents and insectivores captured in Wielkopolska region. Wiad Parazytol. 2001; 47 (supl.2): 29.
Murakoshi F, Fukuda Y, Matsubara R, Kato Y, Sato R, Sasaki T, Tada C, Nakai Y. Detection and genotyping of Cryptosporidium spp. in large Japanese field mice, Apodemus speciosus. (access: 2013.02.011).
Cieloszyk J, Goni P, García A, Remacha MA, Sánchez E, Clavel A. Two cases of zoonotic cryptosporidiosis in Spain by the unusual species Cryptosporidium ubiquitum and Cryptosporidium felis. (access: 2012.04.11).
Stanko M. Bionomics and ecology of Apodemus agrarius (Pall.) (Rodentia: Muridae) on Vychodoslovenska Nizina lowland. II. Population structure and density. Biologia, Bratislava 1994; 49: 797–805.