BRIEF COMMUNICATION
Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs
1
Department of Animal Pathology, Prophylaxis and Food Hygiene. Faculty of Veterinary Medicine, University of Pisa, Italy
Ann Agric Environ Med. 2013;20(2):289-292
KEYWORDS
Anaplasma phagocytophilumhunting dogsindirect immunofluorescence antibody testpolymerase chain reaction
ABSTRACT
The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, has been reported in Italy in humans and several animal species, including dogs, but data concerning its prevalence in the Italian canine population are lacking. The aim of this study was to determine the prevalence of A. phagocytophilum infection in hunting dogs which live in geographic areas of central Italy where the infection had been previously detected in wild animals. Sera from 215 hunting dogs were tested by indirect immunofluorescence antibody test (IFAT) to detect antibodies to A. phagocytophilum. Buffy coat samples from the same 215 animals were submitted to a polymerase chain reaction (PCR) assay specific for the A. phagocytophilum 16S rRNA gene. Thirty-two (14.8%) dogs proved to be seropositive with antibody titres from 1:40–1:2,560. The highest seroprevalence was observed in dogs 6–10-year-old. Two seropositive (0.9%) animals were also PCR positive. Sequencing of PCR products revealed gene sequences of A. phagocytophilum in both cases. These results confirm the presence of A. phagocytophilum in the Italian areas studied; thus, the zoonotic potential of this agent should be considered particularly for people, as hunters, at risk of exposure to tick bites.
REFERENCES (46)
1.
Dumler JS, Barbet AF, Bekker CP, Dasch GA, Palmer GH, Ray SC, et al. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ‘HE agent’ as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol. 2001; 51: 2145–2165.
2.
De La Fuente J, Naranjo V, Ruiz-Fons F, Vicente J, Estrada-Peña A, Almazán C, et al. Prevalence of tick-borne pathogens in ixodid ticks (Acari: Ixodidae) collected from European wild boars (Sus scrofa) and Iberian red deer (Cervus elaphus hispanicus) in central Spain. Eur J Wildl Res. 2004; 50: 187–196.
3.
Carrade DD, Foley JE, Borjesson DL, Sykes JE. Canine granulocytic anaplasmosis: a review. J Vet Intern Med. 2009; 23: 1129–1141.
4.
Stuen S, Engvall EO, Van de Poll I, Schouls LM. Granulocytic ehrlichiosis in a roe deer calf in Norway. J Wildl Dis. 2001; 37: 614–616.
5.
Liz JS, Sumner JW, Pfister K, Brossard M. PCR detection and serological evidence of granulocytic ehrlichial infection in roe deer (Capreolus capreolus) and chamois (Rupicapra rupicapra). J Clin Microbiol. 2002; 40: 892–897.
6.
Petrovec M., Bidovec A, Summer JW, Nicholson WL, Childs JE, AvsicZupanc T. Infection with Anaplasma phagocytophila in cervids from Slovenia: evidence of two genotypic lineages. Wien Klin Wochenschr. 2002; 114: 641–647.
7.
Portillo A, Pérez-Martínez L, Santibáñez S, Santibáñez P, Palomar AM, Oteo JA. Anaplasma spp. in wild mammals and Ixodes ricinus from the North of Spain. Vector Borne Zoonotic Dis. 2011; 11: 3–8.
8.
Ebani VV, Cerri D, Fratini F, Ampola M, Andreani E. Anaplasma phagocytophilum infection in a fallow deer (Dama dama) population in a preserve of central Italy. New Microbiol. 2007; 30: 161–165.
9.
Veronesi F, Galuppi R, Tampieri MP, Botoli C, Mammoli R, Piergili Fioretti D. Prevalence of Anaplasma phagocytophilum in fallow deer (Dama dama) and feeding ticks from an Italy preserve. Res Vet Sci. 2011; 90: 40–43.
10.
Ebani VV, Verin R, Fratini F, Poli A, Cerri D. Molecular survey of Anaplasma phagocytophilum and Ehrlichia canis in red foxes (Vulpes vulpes) from Central Italy. J Wildl Dis. 2011; 47: 699–703.
11.
Madewell B, Gribble D. Infection in two dogs with an agent resembling Ehrlichia equi. J Am Vet Med Assoc. 1982; 180: 512–514.
12.
Greig B, Asanovich KM, Armstrong PJ. Geografic, clinical, serological, and molecular evidence of granulocytic ehrlichiosis, a likely zoonotic disease, in Minnesota and Winsconsin dogs. J Clin Microbiol. 1996; 34: 44–48.
13.
Egenvall A, Hedhammar A, Bjoersdorff A. Clinical features and serology of 14 dogs affected by granulocytic ehrlichiosis in Sweden. Vet Rec. 1997; 140: 222–226.
14.
Cohn LA. Ehrlichiosis and related infections. Vet Clin North Am Small Anim Pract. 2003; 33: 863–884.
15.
Lester SJ, Breitschwerdt EB, Collis CD, Hegartry BC. Anaplasma phagocytophilum infection (granulocytic anaplasmosis) in a dog from Vancouver Island. Can Vet J. 2005; 46: 825–827.
16.
Poitout FM, Shinozaki JK, Stockwell PJ, Holland CT, Shukla SK. Genetic variants of Anaplasma phagocytophilum infecting dogs in western Washington State. J Clin Microbiol. 2005, 43: 796–801.
17.
Kohn B, Galke D, Beelitz P, Pfister K. Clinical features of canine granulocytic ehrlichiosis in 18 naturally infected dogs. J Vet Intern Med. 2008; 22: 1289–1295.
18.
Cockwill KR, Taylor SM, Snead ECR, Dickinson R, Cosford K, Malek S. et al. Granulocytic anaplasmosis in three dogs from Saskatoon, Saskatchewan. Can Vet J. 2009; 50: 835–840.
19.
Goldman EE, Breitschwerdt EB, Grindem CB, Hegarty BC, Walls JJ, Dumler JS. Granulocytic ehrlichiosis in dogs from North Carolina and Virginia. J Vet Intern Med. 1998; 12: 61–70.
20.
Shaw SE, Binns SH, Birtles RJ, Day MJ, Smithson R, Kenny MJ. Molecular evidence of tick-transmitted infections in dogs and cats in the United Kingdom. Vet Rec. 2005; 157: 645–648.
21.
De la Fuente J, Torina A, Naranjo V, Nicosia S, Alongi A, LaMantia F, Kocan KM. Molecular characterization of Anaplsma platys strains from dogs in Sicily, Italy. BMC Vet Res. 2006; 2: 24.
22.
Solano-Gallego L, Trotta M, Razia L, Furlanello T, Caldin M. Molecular survey of Ehrlichia canis and Anaplasma phagocytophilum from blood of dogs in Italy. Ann NY Acad Sci. 2006; 1078: 515–518.
23.
Jensen J, Simon D, Escobar HM, Soller JT, Bullerdiek J, Beelitz P. et al. Anaplasma phagocytophilum in dogs in Germany. Zoonoses Public Health. 2007; 54: 94–101.
24.
Santos AS, Alexandre N, Sousa R, Nuncio MS, Bacellar F, Dumler JS. Serological and molecular survey of Anaplasma species infection in dogs with suspected tick-borne disease in Portugal. Vet Rec. 2009; 164: 168–171.
25.
Zygner W, Gorski P, Wedrychowicz H. Detection of the DNA of Borrelia afzelii, Anaplasma phagocytophilum and Babesia canis in blood samples from dogs in Warsaw. Vet Rec. 2009; 164: 465–467.
26.
Ruscio M, Cinco M. Human granulocytic ehrlichiosis in Italy. Ann NY Acad Sci. 2003; 990: 350–352.
27.
Manna L, Alberti A, Pavone LM, Scibelli A, Staiano N, Gravino AE. First molecular characterization of a granulocytic ehrlichia strain isolated from a dog in south Italy. Vet J. 2004; 167: 224–227.
28.
Alberti A, Zobba R, Chessa B, Addis MF, Sparagano O, Pinna Parpaglia ML, et al. Equine and canine Anaplasma phagocytophilum strains isolated on the island of Sardinia (Italy) are phylogenetically related to pathogenic strains from the United States. Appl Environ Microbiol. 2005; 71: 6418–6422.
29.
Lillini E, Macrì G, Proietti G, Scarpulla M. New findings on anaplasmosis caused by infection with Anaplasma phagocytophilum. Ann NY Acad Sci. 2006; 1081: 360–370.
30.
Torina A, Caracappa S. Dog tick-borne diseases in Sicily, Italy. Parassitologia. 2006; 48: 145–147.
31.
Ebani VV, Cerri D, Fratini F, Ampola M, Andreani E. Seroprevalence of Anaplasma phagocytophilum in domestic and wild animals from central Italy. New Microbiol. 2008; 31: 371–375.
32.
Massung RF, Slater K, Owens JH, Nicholson WL, Mather TN, Solberg VB, Olson JG. Nested PCR assay for detection of granulocytic Ehrlichiae. J Clin Microbiol. 1998; 36: 1090–1095.
33.
Pusterla N, Pusterla JB, Deplazes P, Wolfensberger C, Müller W, Hörauf A, et al. Seroprevalence of Ehrlichia canis and canine granulocytic Ehrlichia infection in dogs in Switzerland. J Clin Microbiol. 1998; 36: 3460–3462.
34.
Egenvall A, Bonnett BN, Gunnarsson A, Hedhammar A, Shoukri M, Bornstein S, Artursson K. Seroprevalence of granulocytic Ehrlichia spp. and Borrelia burgdorferi sensu lato in Swedish dogs 1991–94. Scand J Infect Dis. 2000; 32: 19–25.
35.
Barutzki D, De Nicola A, Zeziola M, Reule M. Seroprevalence of Anaplasma phagocytophilum infection in dogs in Germany. Berl Munch Tierarztl Wochenschr. 2006; 119: 342–347.
36.
Solano-Gallego L, Llull J, Osso M, Hegarty B, Breitschwerdt E. A serological study of exposure to arthropod-borne pathogens in dogs from northeastern Spain. Vet Res. 2006; 37: 231–244.
37.
Amusategui I, Tesouro MA, Kakoma I, Sainz A. Serological reactivity to Ehrlichia canis, Anaplasma phagocytophilum, Neorickettsia risticii, Borrelia burgdorferi and Rickettsia conorii in dogs from northwestern Spain. Vector Borne Zoonotic Dis. 2008; 8: 797–803.
38.
Bertolotti L, Tomassone L, Tramuta C, Grego E, Amore G, Ambrogi C, Nebbia P, Mannelli A. Borrelia lusitaniae and Spotted Fever Group rickettsiae in Ixodes ricinus (Acari: Ixodidae) in Tuscany, central Italy. J Med Entomol. 2006; 43: 159–165.
39.
Cassini R, Zanutto S, Frangipane di Regalbono A, Gabrielli S, Calderini P, et al. Canine piroplasmosis in Italy: epidemiological aspects in vertebrate and invertebrate hosts. Vet Parasitol. 2009; 165: 30–35.
40.
Iori A, Gabrielli S, Calderini P, Moretti A, Pietrobelli M, Tampieri MP, et al. Tick reservoirs for piroplasms in central and northern Italy. Vet Parasitol. 2010; 170: 291–296.
41.
Ragagli C, Bertolotti L, Giacobini M, Mannelli A, Bisanzio D, Amore G, Tomassone L. Transmission dynamics of Borrelia lusitaniae and Borrelia afzelii among Ixodes ricinus, lizards, and mice in Tuscany, central Italy. Vector Borne Zoonotic Dis. 2011; 11: 21–28.
42.
Santino I, Del Piano M, Sessa R, Favia G, Iori A. Detection of four Borrelia burgdorferi genospecies and first report of human granulocytic ehrlichiosis agent in Ixodes ricinus ticks collected in central Italy. Epidemiol Infect. 2002; 129: 93–97.
43.
Cinco M, Padovan D, Murgia R, Heldtander M, Engvall EO. Detection of HE agent-like Ehrlichia in Ixodes ricinus ticks in northern Italy by PCR. Wien Klin Wochenschr. 1998; 110: 898–900.
44.
Mantelli B, Pecchioli E, Hauffe HC, Rosà R. Prevalence of Borrelia burgdorferi s.l. and Anaplasma phagocytophilum in the wood tick Ixodes ricinus in the Province of Trento, Italy. Eur J Clin Microbiol Infect Dis. 2006; 25: 737–739.
45.
Carpi G, Bertolotti L., Pecchioli E., Cagnacci F., Rizzoli A. Anaplasma phagocytophilum groEL gene heteroneity in Ixodes ricinus larvae feeding on roe deer in Northeastern Italy. Vector Borne Zoonotic Dis. 2009; 9: 179–184.
46.
Skotarezak B, Adamska M, Rymaszewska A, Supron M, Sawczuk M, Maciejewska A. Anaplasma phagocytophila and protozoans of Babesia genus in dogs from endemic areas of Lyme disease in north-western Poland. Wiad Parazytol. 2004; 50: 555–561.
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