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First report of Enterocytozoon bieneusi and Encephalitozoon intestinalis infection of wild mice in Slovakia
 
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1
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
2
Department of Zoology, Slovak Academy of Science, Košice, Slovak Republic
 
Ann Agric Environ Med. 2015;22(2):251–252
KEYWORDS
ABSTRACT
Increased risk of zoonotic transmission of the potential human pathogenic species Enterocytozoon bieneusi, Encephalitozoon intestinalis and Encephalitozoon cuniculi was detected in wild immunocompetent mice (Mus musculus musculus; n=280). Analysis was conducted with the use of PMP1/PMP2 primers and SYBR Green RT-PCR. Using Real Time PCR and comparing the sequences with sequences in the GenBank, E. bieneusi was detected in 3 samples (1.07 %), E. cuniculi in 1 sample (0.35 %) and E. intestinalis in 1 sample (0.35 %). The results of this report document the low host specificity of detected microsporidia species, and imply the importance of synanthropic rodents as a potential source of human microsporidial infection.
ACKNOWLEDGEMENTS
The study has supported by the Grant VEGA, No. 1/0390/12, 1/0063/13.
 
REFERENCES (12)
1.
Izquierdo F, Castro Hermida JA, Fenoy S, Mezo M, Gonzalez-Warleta M. Detection of microsporidia in drinking water, wastewater and recreational rivers. Water Res. 2011; 45: 4837–4843.
 
2.
Didier ES, Weiss LM. Microsporidiosis: current status. Curr Opin Infect Dis. 2006; 19: 485–492.
 
3.
Halánová M, Letková V, Macák V, Štefkovič M, Halán M. The first finding of antibodies to Encephalitozoon cuniculi in cows in Slovakia. Vet Parasitol. 1999; 82(2): 167–171.
 
4.
Malčeková B, Halánová M, Sulínová Z, Molnár L, Ravaszová P, Adam J, Halán M, Valocký I, Baranovič M. Seroprevalence of antibodies to Encephalitozoon cuniculi and Encephalitozoon intestinalis in humans and animals. Res Vet Sci. 2010; 89(3): 358–361.
 
5.
Sak B, Kváč M, Květoňová D, Albrecht T, Piálek J. The first report on natural Enterocytozoon bieneusi and Encephalitozoon spp. infections in wild East-European House Mice (Mus musculus musculus) and West-European House Mice (M. m. domesticus) in a hybrid zone across the Czech Republic-Germany border. Vet Parasitol. 2011; 178(3–4): 246–250.
 
6.
Malčeková B, Valenčáková A, Molnár L, Kočišová A. First detection and genotyping of huma – associated microsporidia in wild waterfowl of Slovakia. Acta Parasitol. 2013; 58(1): 13–17.
 
7.
Visvesvara GS, Moura H, Leitch GJ, Schwartz DA. Culture and propagation of microsporidia. In: Wittner M (ed.). Microsporidia and microsporidiosis. Washington, D.C, ASM Press, 1999.p.363–392.
 
8.
Canning EU, Lom J. The Microsporidia of Vertebrates . London and New York, Academic Press, Inc.1986.
 
9.
Wasson K, Peper RL. Mammalian microsporidiosis. Vet Pathol. 2000; 37: 113–128.
 
10.
Valenčáková A, Bálent P, Ravaszová P, Horák A, Oborník M, Halánová M, Malčeková B, Novotný F, Goldová M. Molecular identification and genotyping of Microsporidia in selected hosts. Parasitol Res. 2012; 110: 689–693.
 
11.
Müller-Doblies UU, Herzog K, Tanner I, Mathis A, Deplazes P. First isolation and characterisation of Encephalitozoon cuniculi from a free-ranging rat (Rattus norvegicus). Vet Parasitol. 2002; 107(4): 279–285.
 
12.
Cama VA, Pearson J, Cabrera L, Pacheco L, Gilman R, Meyer S, Ortega Y, Xiao L. Transmission of Enterocytozoon bieneusi between a child and guinea pigs. J Clin Microbiol. 2007; 45(8): 2708–2710.
 
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