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First report of [i]Enterocytozoon[/i] bieneusi and [i]Encephalitozoon intestinalis[/i] infection of wild mice in Slovakia

Michal Stanko 2,  
 
1
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
2
Department of Zoology, Slovak Academy of Science, Košice, Slovak Republic
Ann Agric Environ Med 2015;22(2):251–252
KEYWORDS:
ABSTRACT:
Increased risk of zoonotic transmission of the potential human pathogenic species [i]Enterocytozoon bieneusi[/i], [i]Encephalitozoon intestinalis[/i] and [i]Encephalitozoon cuniculi [/i]was detected in wild immunocompetent mice (Mus musculus musculus; n=280). Analysis was conducted with the use of PMP1/PMP2 primers and SYBR Green RT-PCR. Using Real Time PCR and comparing the sequences with sequences in the GenBank, [i]E. bieneusi[/i] was detected in 3 samples (1.07 %), [i]E. cuniculi [/i]in 1 sample (0.35 %) and [i]E. intestinalis[/i] in 1 sample (0.35 %). The results of this report document the low host specificity of detected microsporidia species, and imply the importance of synanthropic rodents as a potential source of human microsporidial infection.
eISSN:1898-2263
ISSN:1232-1966