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RESEARCH PAPER
 
 

Detection and identification of six Cryptospordium species in livestock in Slovakia by amplification of SSU and GP60 genes with the use of PCR analysis

 
1
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
Ann Agric Environ Med 2016;23(2):254–258
KEYWORDS:
ABSTRACT:
[b]Introduction.[/b] In this study we examined 200 faecal samples from pigs and calves with suspected cryptosporidiosis were examined by the PCR methods: nested PCR for amplification of SSU region; nested PCR for amplification of GP60 region; and with restriction analysis of DNA (PCR-RFLP). The sequencing identified the following species: [i]Cryptosporidium muris[/i] (2), [i]Cryptosporidium andersoni[/i] (1), [i]Cryptosporidium bovis[/i] (4), [i]Cryptosporidium suis[/i] (2), [i]Cryptosporidium scrofarum[/i] (10), mixed infection caused by [i]C. scrofarum[/i] and [i]C. muris[/i] (1),and [i]Cryptosporidium parvum[/i] (10) genotype A subtype IIaA17G2R1. [b]Results and conclusions.[/b] The findings suggest that livestock can be an important source of zoonotic species or genotypes of [i]Cryptosporidium[/i], which may adversely affect the public health of human populations. This is the first time in our country that the [i]Cryptosporidium[/i] species has been identified in livestock in Slovakia. The identification and genotyping of this pathogen in Slovakia, completes the epidemiological situation in Europe for [i]Cryptosporidum[/i] species.
eISSN:1898-2263
ISSN:1232-1966