BRIEF COMMUNICATION
Cryptosporidium spp. in dogs and cats in Poland
1
Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Division of Parasitology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Poland
Ann Agric Environ Med. 2021;28(2):345-347
KEYWORDS
TOPICS
Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases)Prevention of occupational diseases in agriculture, forestry, food industry and wood industry
ABSTRACT
Introduction:
Cryptosporidium spp. are common protozoan parasites of animals and humans. Due to their zoonotic potential it is important to know their species and prevalence in dogs and cats.
Objective:
The aim of the study was to determine the occurrence and molecular characteristics of Cryptosporidium spp. in dogs and cats in Poland
Material and Methods:
A total of 365 faecal samples (264 dogs and 101 cats) collected from animals living in Poland were analyzed using the Ziehl-Neelsen staining method and genus-specific PCR assay to amplify the Cryptosporidium 18S rRNA gene.
Results:
Cryptosporidium were found in 11 out of the 365 examined stool samples (3%). PCR analysis identified Cryptosporidium in 9 out of 264 canine stool samples (3.4%) and 2 out of 101 feline specimens (2%). DNA sequencing confirmed the presence of C. canis and C. parvum in dogs and C. felis in cats.
Conclusions:
This is the first molecular characterization of Cryptosporidium spp. infection in dogs and cats in Poland.
Cryptosporidium spp. are common protozoan parasites of animals and humans. Due to their zoonotic potential it is important to know their species and prevalence in dogs and cats.
Objective:
The aim of the study was to determine the occurrence and molecular characteristics of Cryptosporidium spp. in dogs and cats in Poland
Material and Methods:
A total of 365 faecal samples (264 dogs and 101 cats) collected from animals living in Poland were analyzed using the Ziehl-Neelsen staining method and genus-specific PCR assay to amplify the Cryptosporidium 18S rRNA gene.
Results:
Cryptosporidium were found in 11 out of the 365 examined stool samples (3%). PCR analysis identified Cryptosporidium in 9 out of 264 canine stool samples (3.4%) and 2 out of 101 feline specimens (2%). DNA sequencing confirmed the presence of C. canis and C. parvum in dogs and C. felis in cats.
Conclusions:
This is the first molecular characterization of Cryptosporidium spp. infection in dogs and cats in Poland.
ACKNOWLEDGEMENTS
This research was supported by statutory research and development activity founds by Minister of Science and Higher Education assigned to Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences,
Poland.
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
REFERENCES (26)
1.
Ryan U. Cryptosporidium in birds, fish and amphibians. Exp Parasitol. 2010; 124, 113–120.
2.
Uehlinger, FD, Greenwood SJ, McClurea JT. Zoonotic potential of Giardia duodenalis and Cryptosporidium spp. and prevalence of intestinal parasites in young dogs from different populations on Prince Edward Island, Canada. Vet Parasitol. 2013; 196: 509–514.
3.
Alves MEM, Martins FD, Bräunig P, Pivoto FL, Sangioni LA, Vogel, FSF. Molecular Detection of Cryptosporidium spp. and the occurrence of intestinal parasites in fecal samples of Naturally infected dogs and cats. Parasitol Res. 2018; 117: 3033–3038.
4.
Yang R, Jie Ying J L, Monis P, Ryan U. Molecular characterisation of Cryptosporidium and Giardia in cats (Felis catus) in Western Australia. Exp Parasitol. 2015; 155: 13–18.
5.
Li W, Li Y, Song M, et al. Prevalence and genetic characteristics of Cryptosporidium, Enterocytozoon bieneusi and Giardia duodenalis in cats and dogs in Heilongjiang province, China. Vet Parasitol. 2015; 208: 125–134.
6.
Rosanowski SM, Banica M, Ellis E. The molecular characterization of Cryptosporidium species in relinquished dogs in Great Britain: a novel zoonotic risk? Parasitol Res. 2018; 117: 1663–1667.
7.
Xu H, Jin Y, Wu W, et al. Genotypes of Cryptosporidium spp., Enterocytozoon bieneusi and Giardia duodenalis in dogs and cats in Shanghai, China. Parasit Vectors. 2016; 9: 121.
8.
Paoletti B, Traversa D, Iorio R. Zoonotic parasites in feces and fur of stray and private dogs from Italy. Parasitol Res 2015; 114: 2135–2141.
9.
Kostopoulou D, Claerebout E, Arvanitis D, Ligda P, Voutzourakis N, Casaert S, Sotiraki S. Abundance, zoonotic potential and risk factors of intestinal parasitism amongst dog and cat populations: The scenario of Crete, Greece. Parasit Vectors. 2017; 10: 43.
10.
Hinney B, Ederer C, Stengl C, Wilding K, Štrkolcová G, Harl J, et al. Enteric protozoa of cats and their zoonotic potential—a field study from Austria. Parasitol Res. 2015; 114: 2003–2006.
11.
de Lucio A, Bailo B, Aguilera M, Cardona GA, Fernández-Crespo JC, Carmena D. No molecular epidemiological evidence supporting household transmission of zoonotic Giardia duodenalis and Cryptosporidium spp. from pet dogs and cats in the province of Álava, Northern Spain. Acta Trop. 2017; 170: 48–56.
12.
Gundłach JL, Sadzikowski AB, Studzińska MB, Tomczuk K. Invasion of Giardia spp. and Cryptosporidium spp. in dogs and cats. Med Weter. 2004; 60 (11): 1202–1203.
13.
Bajer A, Bednarska M. Cryptosporidium spp. and Giardia spp. infections in sled dogs. Med Weter. 2007; 63: 681–7.
14.
Ryan U, Xiao L, Read C, Zhou L, Lal AA, Pavlasek I. Identification of Novel Cryptosporidium Genotypes from the Czech Republic. Appl Environ Microbiol. 2003; 69:7 4302–4307.
15.
Cirak VY, Bauer C. Comparison of conventional coproscopical methods and commercial coproantigen ELISA kits for the detection of Giardia and Cryptosporidium infections in dogs and cats. Berl Munch Tierarztl Wochenschr. 2004; 117(9–10): 410–413.
16.
Osman M, Bories J, El Safadi D, Poirel MT, Gantois N, Benamrouz-Vanneste, et al. Prevalence and genetic diversity of the intestinal parasites Blastocystis sp. and Cryptosporidium spp. in household dogs in France and evaluation of zoonotic transmission risk. Vet Parasitol. 2015; 214: 167–170.
17.
Gil H, Cano L, de Lucio A, Bailo B, de Mingo MH, Cardona GA. Detection and molecular diversity of Giardia duodenalis and Cryptosporidium spp. in sheltered dogs and cats in Northern Spain. Infect Genet Evol. 2017; 50: 62–69.
18.
Batchelor DJ, Tzannes S, Graham PA, Wastling J, Pinchbeck GL, German AJ. Detection of Endoparasites with Zoonotic Potential in Dogs with Gastrointestinal Disease in the UK. Transbound Emerg Dis. 2008; 55: 99–104.
19.
Khan A, Shaik J, Grigg ME. Genomics and molecular epidemiology of Cryptosporidium species. Acta Trop. 2018; 184: 1–14.
20.
Sannella AR, Suputtamongkol Y, Wongsawat E, Cacció SM. A retrospective molecular study of Cryptosporidium species and genotypes in HIV-infected patients from Thailand. Parasit Vectors. 2019; 12(1): 91.
21.
Sotiriadou I, Pantchev N, Gassmann D, Karanis P. Molecular identification of Giardia and Cryptosporidium from dogs and cats. Parasite. 2013; 20: 8.
22.
Ayinmode AB, Obebe OO, Falohun OO. Molecular detection of Cryptosporidium species in street sampled dog faeces in Ibadan, Nigeria. Vet Parasitol Reg Stud Reports. 2018; 14: 54–58.
23.
Kváč M, Hofmannová L, Ortega Y, Holubová N, Horčičková M, Kicia M. Stray cats are more frequently infected with zoonotic protists than pet cats. Folia Parasitol. 2017; 64: 034.
24.
Šlapeta J. Cryptosporidiosis and Cryptosporidium species in animals and humans: a thirty colour rainbow? Int J Parasitol. 2013; 43: 957–970.
25.
Aghamolaie S, Rostami A, Fallahi SH, Tahvildar Biderouni F, Haghighi A, Salehi N. Evaluation of modified Ziehl–Neelsen, direct fluorescentantibody and PCR assay for detection of Cryptosporidium spp. in children faecal specimens. J Parasit Dis. 2016; 40(3): 958–963.
26.
Brar APS, Sood NK, Kaur P, Singla LD, Sandhu BS, Gupta K. Periurban outbreaks of bovine calf scours in Northern India caused by Cryptosporidium in association with other enteropathogens. Epidemiol Infect. 2017; 145: 2717–2726.
Share
RELATED ARTICLE
| eISSN: | 1898-2263 |
| ISSN: | 1232-1966 |
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
© 2006-2024 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
