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RESEARCH PAPER
 
CC BY-NC-ND 3.0
 
 

Chemometric evaluation of concentrations of trace elements in intervertebral disc tissue in patient with degenerative disc disease

Łukasz Kubaszewski 1, 2  ,  
Zuzanna Gasik 4,  
 
1
Department of Orthopaedic and Traumatology, W. Dega University Hospital, University of Medical Sciences, Poznań, Poland
2
Clinic; Polyclinic of Neuroorthopedic and Neurology, Institute of Rheumatology, Warsaw, Poland
3
Faculty of Chemistry, Adam Mickiewicz University in Poznań, Poland
4
Clinic and Polyclinic of Neuro-orthopedic and Neurology, Institute of Rheumatology, Warsaw, Poland
5
Department of Spondyloorthopaedics and Biomechanics of the Spine, Poznań University of Medical Sciences, Poland
KEYWORDS:
ABSTRACT:
Background:
The work is designed to uncover the pattern of mutual relation among trace elements and epidemiological data in the degenerated intervertebral disk tissue in humans. Hitherto the reason of the degenerative process is not fully understood. Trace elements are the basic components of the biological compound related both its metabolism as well as environmental exposure. The relation pattern among elements occurs gives new perspective in solving the cause of the disease.

Material and Methods:
We have analysed trace elements content in the 30 intervertebral disc from 22 patients with degenerative disc disease. The concentrations of Al, Cu, Cd, Mo, Ni and Pb were determined with Atomic Absorption Spectrometry. To analyse the multidimentional relation between trace element concentration and epidemiological data the chemometric analysis was applied.

Results:
The similarity have been shown in occurrence of following pairs: Cd-Mo as well as Mg-Zn. The second pair was correlated with Pb concentration. Pb levels are observed to be competitive to Cu concentration. Cd concentration was related to Zn and Mg deficiency. No single but rather cluster of epidemiological data show observable influence on the TE tissue variance. Zn and Cu was related to the male sex. Operation with orthopedic implants were related to combined Al, Mo and Zn concentration.

Conclusions:
This is the first chemometric analysis of trace elements in disk tissue. It shows multidimentional relations that are missed by the classical statistic. The analysis shows significant relation. The nature of the relations is the basis for further metabolic and environmental research

CORRESPONDING AUTHOR:
Łukasz Kubaszewski   
Department of Orthopaedic and Traumatology, W. Dega University Hospital, University of Medical Sciences, Poznań, Poland
 
REFERENCES (26):
1. Holmberg S, Thelin A, Stiernström EL, Svärdsudd K. Low back pain comorbidity among male farmers and rural referents: a population-based study. Ann Agric Environ Med. 2005; 12(2): 261–268.
2. Taher F, Essiq D, Lebl DR, Hughes AP, Sama AA, Cammisa FP, Girardi FP. Lumbar degenerative disc disease: current and future concepts of diagnosis and management. Adv Orthop. 2012; doi: 10.1155/2012/970752.
3. Kabata-Pendias A, Mukherjee AB. Trace Elements from Soil to Human. Springer-Verlag Berlin Heidelberg, 2007.
4. Longo UG, Maffulli N, Denaro V, Petrillo S, Franceschetti E. Growth Factors and Anticatabolic Substances for Prevention and Management of Intervertebral Disc Degeneration. Stem Cells Int. 2012; doi:10.1155/2012/897183.
5. Birkedal-Hansen H, Moore WG, Bodden MK, Windsor LJ, Birkedal-Hansen B, DeCarlo A, Engler JA. Matrix metalloproteinases: a critival review. Oral Biol Med. 1993; 4(2):197–250.
6. Cai L, Li XK, Song Y, Cherian MG. Essentiality, toxicology and chelation therapy of zinc and copper. Curr Med Chem 2005; 12: 2753–2763.
7. Gemperline P, editor. Practical Guide To Chemometrics. 2nd ed. CRC Press(FL), 2006.
8. Brodziak B, Kwapuliński J, Bogunia M, Kropczyńska A, Orczyk H, Paukszto A, Nogaj E. The Occurrence of Co, Ag, Al, Sr in Femur Head. Polish J Environ Stud. 2006; 15: 20–22.
9. Tohno S, Tohno Y, Minami T, Ichii M, Okazaki Y, Utsumi M, Nishiwaki F, Yamada M. Difference of mineral contents in human intervertebral disks and its age-related change. Biol Trace Elem Res. 1996; 52(2):117–124.
10. Minami T, Hashii K, Tateyama I, Kadota E, Tohno Y, Tohno S, Utsumi M, Yamado MO, Ichii M, Namikawa K, et al. Accumulation of platinum in the intervertebral discs and vertebrae of ovarian tumor-bearing patients treated with cisplatin. Biol Trace Elem Res. 1994; 42(3): 253–257.
11. Pfirrmann CW, Metzdorf A, Zanetti M, Hodler J, Boos N. Magnetic resonance classification of lumbar intervertebral disc degeneration. Spine. 2001; 26(17): 1873–1878.
12. Cohen AR, Trotzky MS, Pincus D. Reassessment of the Microcytic Anemia of Lead Poisoning. Pediatrics. 1981; 67(6): 904–906.
13. Rude RK. Magnesium. In: Ross AC, Caballero B, Cousins RJ, Tucker KL, Ziegler TR, (eds.) Modern Nutrition in Health and Disease, 11th ed. Lippincott Williams & Wilkins; Baltimore 2012. p.159–175.
14. Witkowski M, Hubert J, Mazur A. Methods of assessment of magnesium status in humans: a systematic review. Magnesium Res. 2011; 24: 163–180.
15. Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc. Washington (D.C.) National Academy Press, 2002.
16. Prasad AS. Zinc: an overview. Nutrition. 1995; 11: 93–99.
17. Milbury, Paul E, Richer, Alice C. Understanding the Antioxidant Controversy. Wesport (CT) Greenwood Publishing Group. 2008.
18. Keen CL, Gershwin ME. Zinc deficiency and immune function. Annu Rev Nutr. 1990; 10: 415–431. doi:10.1146/annurev.nu.10.070190.002215.
19. Gross J, Lapiere CM. Collagenolytic activity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci. USA. 1962; 48: 1014–1022.
20. Van Lint P, Libert C. Chemokine and cytokine processing by matrix metalloproteinases and its effect on leukocyte migration and inflammation. J Leukoc Biol. 2007; 82(6): 1375–1381. doi:10.1189/jlb.0607338.
21. Swandulla D, Armstrong CM. Calcium channel block by cadmium in chicken sensory neurons. Proc Natl Acad Sci. 1989. 86 (5): 1736–1740. doi:10.1073/pnas.86.5.1736.
22. Scott C, Lyons TW, Bekker A, Shen Y, Poulton SW, Chu X, Anbar AD. Tracing the stepwise oxygenation of the Proterozoic ocean. Nature. 2008; 452(7186): 456–459. doi: 10.1038/nature06811.
23. Emsley J. Nature‘s Building Blocks. Oxford: Oxford (UK) Oxford University Press, 2011.
24. Lippard SJ, Berg JM. Principles of bioinorganic chemistry. Mill Valley (CA) University Science Books, 1994.
25. Li Y-H. A compendium of geochemistry: from solar nebula to the human brain. Princeton (NJ) Princeton Univ Press, 2000.
26. Kretzer JP, Reinders J, Sonntag R, Hagmann S, Streit M, Jeager S, Moradi B. Wear in total knee arthroplasty-just a question of polyethylene?: Metal ion release in total knee arthroplasty. Int Orthop. 2013; 12 [Epub ahead of print].
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