RESEARCH PAPER
Characterization of the heat-released substance from rat ileal muscle.
 
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Department of Pharmacology, Faculty of Medicine and Allied Sciences, and Heat Stroke Research Unit, King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Saudi Arabia
CORRESPONDING AUTHOR
Osama Tayeb
Department of Pharmacology, Faculty of Medicine & Allied Sciences, King Abdulaziz University, P.O. Box 9029, Jeddah 21413, Saudi Arabia
 
Ann Agric Environ Med. 1999;6(2):99–103
 
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ABSTRACT
Previous studies have proposed that increasing the temperature around the rat isolated ileal muscles induces a heat-released substance (HRS) which causes a decrease in the contractile response. Therefore, we have studied various characteristics of this proposed HRS in animals exposed to heat-stress conditions. In these animals, the contraction of muscles in response to carbachol was decreased by increasing the temperature from 37 degrees C to 40 degrees C. Further, bathing the ileum in a conditioned medium prepared by incubation of the ileal muscle at 40 degrees C has shown less contraction at 40 degrees C than in presence of normal Krebs medium. In addition, conditioned mediums, prepared at 40 degrees C were run on SDS-polyacrylamide gel electrophoresis and have shown the presence of a distinct protein band at a m.w. region of 55 kDa. These results confirm our previous studies that increasing the temperature around the muscles induces the HRS that causes the decrease in the contractile response.
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ISSN:1232-1966