RESEARCH PAPER
Assessment of the risk for human health of Enterovirus and Hepatitis A virus in clinical and water sources from three metropolitan cities of Pakistan
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1
National university of Sciences and Tehnology, Islamabad, Pakistan
 
2
University of Hail, Saudi Arabia
 
3
Department of Zoology, University of the Punjab, Lahore
 
 
Corresponding author
Tahir Ahmad   

National university of Sciences and Tehnology, H-12, 44000 Islamabad, Pakistan
 
 
Ann Agric Environ Med. 2018;25(4):708-713
 
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Molecular studies have confirmed the silent circulation of enterovirus (EntV) and hepatitis A virus in the environment, even in the absence of clinical manifestation. Viral pathogens are among the major causes of disease outbreaks, particularly in the bigger cities and both in the developed and underdeveloped nations.

Material and methods:
Between June 2016 – June 2017, 97 samples of drinking water, river water polluted with sewage and blood were selected and obtained from high risk communities in Pakistan. Negatively charged membrane filters were used to concentrate the virus, followed by the use of specific PCR primers set for quick identification of the waterborne viruses.

Results:
Enteroviruses were recovered from 40%, 28.57% and 33.33% of river water polluted with sewage samples in Lahore, Islamabad and Rawalpindi, respectively, while the presence of 13.13% and 11.76% of viral load was also confirmed in the drinking water of Lahore and Rawalpindi, respectively. A high prevalence of HAV (12.5% and 21.05%) was also verified in the clinical samples. Phylogenetic analysis indicated close resemblance of HAV isolates with the Indian strains. This study is the first ever comparative analysis of the EntV and HAV isolated from environmental samples and clinical specimen on a molecular level.

Conclusions:
The parallel surveillance of EntV and HAV in the river water polluted with sewage, and clinical samples is quite helpful for controlling and reducing the disease burden of the waterborne illnesses.

 
REFERENCES (32)
1.
Nishizawa T, Okamoto H, Konishi K, Yoshizawa H, Miyakawa Y, Mayumi M. A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochem Biophys Res Co. 1997; 241(1): 92–7.
 
2.
Okamoto H, Nishizawa T, Kato N, Ukita M, Ikeda H, Iizuka H, et al. Molecular cloning and characterization of a novel DNA virus (TTV) associated with posttransfusion hepatitis of unknown etiology1. Hepatol Res. 1998; 10(1): 1–16.
 
3.
Miyata H, Tsunoda H, Kazi A, Yamada A, Khan MA, Murakami J, et al. Identification of a novel GC-rich 113-nucleotide region to complete the circular, single-stranded DNA genome of TT virus, the first human circovirus. J Virol. 1999; 73(5): 3582–6.
 
4.
Biagini P, Gallian P, Attoui H, Cantaloube J-F, de Micco P, de Lamballerie X. Determination and phylogenetic analysis of partial sequences from TT virus isolates. J Gen Virol. 1999; 80(2): 419–24.
 
5.
Kowada K, Takeuchi K, Hirano E, Toho M, Sada K. Development of a multiplex real-time PCR assay for detection of human enteric viruses other than norovirus using samples collected from gastroenteritis patients in Fukui Prefecture, Japan. J Med Virol. 2018; 90(1): 67–75.
 
6.
Luo K, He H, Liu Z, Liu D, Xiao H, Jiang X, et al. Novel variants related to TT virus distributed widely in China. J Med Virol. 2002; 67(1): 118–26.
 
7.
Nishizawa T, Okamoto H, Tsuda F, Aikawa T, Sugai Y, Konishi K, et al. Quasispecies of TT virus (TTV) with sequence divergence in hypervariable regions of the capsid protein in chronic TTV infection. J Virol. 1999; 73(11): 9604–8.
 
8.
Leary TP, Erker JC, Chalmers ML, Desai SM, Mushahwar IK. Improved detection systems for TT virus reveal high prevalence in humans, non-human primates and farm animals. J Gen Virol. 1999; 80(8): 2115–20.
 
9.
Vaidya SR, Chitambar SD, Arankalle VA. Polymerase chain reaction-based prevalence of hepatitis A, hepatitis E and TT viruses in sewage from an endemic area. J Hepatol. 2002; 37(1): 131–6.
 
10.
Haramoto E, Katayama H, Oguma K, Yamashita H, Nakajima E, Ohgaki S. One-year monthly monitoring of Torque teno virus (TTV) in wastewater treatment plants in Japan. Water Res. 2005; 39(10): 2008–13.
 
11.
Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees.Mol Biol Evol. 1993; 10(3): 512–26.
 
12.
Cohen J. Statistical power analysis for the behavioral sciences. 2nd. Hillsdale, NJ: erlbaum; 1988.
 
13.
Yu M, Ao Y, Li L, Duan J. Idendification of a novel cosavirus species in the faeces of children and its relationship with acute gastroenteritis in China. Clin Microbiol Infect. 2017; 23: 550–554.
 
14.
Arraj A, Bohatier J, Aumeran C, Bailly J-L, Laveran H, Traoré O. An epidemiological study of enteric viruses in sewage with molecular characterization by RT-PCR and sequence analysis. J Water Health. 2008; 6(3): 351–8.
 
15.
Ahmad T, Anjum S, Afzal MS, Raza H, Zaidi N, Arshad N. Molecular confirmation of enterovirus from sewage and drinking water samples from three cities, Pakistan: a potential risk factor for public health. The Southeast Asian J Trop Med Public Health. 2015; 46(4): 640–9.
 
16.
Espínola EE, Parra GI, Russomando G, Arbiza J. Genetic diversity of the VP4 and VP7 genes affects the genotyping of rotaviruses: analysis of Paraguayan strains. Infect Genet Evol. 2008; 8(1): 94–9.
 
17.
Tan EL, Yong LLG, Quak SH, Yeo WCA, Chow VTK, Poh. Rapid detection of enterovirus 71 by real-time TaqMan RT-PCR. J Clin Virol. 2008; 42(2): 203–6.
 
18.
Tan EL, Chow VTK, Quak SH, Yeo WCA, Poh CL. Development of multiplex real-time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16. Diagn Microbiol Infect Dis. 2008; 61(3): 294–301.
 
19.
Foo DGW, Alonso S, Phoon MC, Ramachandran N, Chow VTK, Poh CL. Identification of neutralizing linear epitopes from the VP1 capsid protein of Enterovirus 71 using synthetic peptides. Virus Res. 2007; 125(1): 61–8.
 
20.
Tufenkeji H. Hepatitis A shifting epidemiology in the Middle East and Africa. Vaccine. 2000;18:S65-S7.
 
21.
Schmidt WP. The elusive effect of water and sanitation on the global burden of disease. Trop Med Int Health. 2014; 19(5): 522–7.
 
22.
Villar LM, Costa MdCEd, Paula VSd, Gaspar AMC. Hepatitis A outbreak in a public school in Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz. 2002; 97(3): 301–5.
 
23.
Khuroo MS, Khuroo MS, Khuroo NS. Transmission of hepatitis E virus in developing countries. Viruses. 2016; 8(9): 253.
 
24.
Zhou F, Kong R, McPhie K, Ratnamohan M, Donovan L, Zeng F, Gilbert GL, Dwyer DE. Identification of 20 common human enterovirus serotypes by the use of a reverse transcription-PCR-based reverse line blot hybridization assay. J Clin Microbiol. 2009; 47(9): 2737–2743.
 
25.
Paula VSd, Baptista ML, Elisabeth L, Christian N, Gaspar AMC. Characterization of Hepatitis A virus isolates from subgenotypes IA and IB in Rio de Janerio, Brazil. J Med Virol. 2002; 66(1): 22–27.
 
26.
Ahmad T, Anjum S, Sadaf Zaidi N, Ali A, Waqas M, Afzal MS. Frequency of hepatitis E and Hepatitis A virus in water sample collected from Faisalabad, Pakistan. Ann Agric Environ Med. 2015; 22(4): 661–4.
 
27.
Monteiro L, Bonnemaison D, Vekris A, Petry KG, Bonnet J, Vidal R, et al. Complex polysaccharides as PCR inhibitors in faeces: Helicobacter pylori model. J Clin Microbiol. 1997; 35(4): 995–8.
 
28.
Baelde H, Cleton-Jansen A, Van Beerendonk H, Namba M, Bovée J, Hogendoorn P. High quality RNA isolation from tumours with low cellularity and high extracellular matrix component for cDNA microarrays: application to chondrosarcoma. J Clin Pathol. 2001; 54(10): 778–82.
 
29.
de Roda Husman AM, Bartram J. Global supply of virus-safe drinking water. Persp in Med Virol. 2007; 17: 127–62.
 
30.
Roberts L. In Pakistan, surveillance for polio reveals a paradox. Science; 2018.
 
31.
Chapron CD, Ballester NA, Fontaine JH, Frades CN, Margolin AB. Detection of astroviruses, enteroviruses, and adenovirus types 40 and 41 in surface waters collected and evaluated by the information collection rule and an integrated cell culture-nested PCR procedure. Appl Environ Microbiol. 2000; 66(6): 2520–5.
 
32.
García-Aljaro C, Martín-Díaz J, Viñas-Balada E, Calero-Cáceres W, Lucena F, Blanch AR. Mobilisation of microbial indicators, microbial source tracking markers and pathogens after rainfall events. Water Res. 2017; 112: 248–53.
 
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