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RESEARCH PAPER
 
 

Age influence on mice lung tissue response to [i]Aspergillus fumigatus[/i] chronic exposure

Marcin Golec 1,  
Marco Chilosi 2,  
Rolf Ziesche 5,  
 
1
Institute of Agricultural Medicine, Lublin, Poland
2
Department of Pathology and Diagnostic, Section of Pathological Anatomy, University of Verona, Verona, Italy
3
Louvain Centre for Toxicology and Applied Pharmacology (LTAP), Institute of Experimental and Clinical Research (IREC), Université Catholique de Louvain, Brussels, Belgium
4
Department of Pneumonology, Oncology and Allergology, Medical University, Lublin, Poland
5
Department of Internal Medicine II, Clinical Division of Pulmonary Medicine, Medical University of Vienna, Vienna, Austria
6
Institute of Agricultural Medicine, Lublin, Poland; Department of Pneumonology, Oncology and Allergology, Medical University, Lublin, Poland
Ann Agric Environ Med 2015;22(1):69–75
KEYWORDS:
ABSTRACT:
[b]Introduction and objective[/b]. Exposure to conidia of [i]Aspergillus fumigatus[/i] was described as a causative factor of a number of the respiratory system diseases, including asthma, chronic eosinophilic pneumonia, hypersensitivity pneumonitis and bronchopulmonary aspergillosis. The study investigates the effects of the repeated exposure to [i]A. fumigatus[/i] in mice pulmonary compartment. Our work tackles two, so far insufficiently addressed, important aspects of interaction between affected organism and[i] A. fumigatus[/i]: 1) recurrent character of exposure (characteristic for pathomechanism of the abovementioned disease states) and 2) impact of aging, potentially important for the differentiation response to an antigen. [b]Materials and methods[/b]. In order to dissect alterations of the immune system involved with both aging and chronic exposure to [i]A. fumigatus[/i], we used 3- and 18-month-old C57BL/6J mice exposed to repeated[i] A. fumigatus[/i] inhalations for 7 and 28 days. Changes in lung tissue were monitored by histological and biochemical evaluation. Concentration of pro- and anti-inflammatory cytokines in lung homogenates was assessed by ELISA tests. [b]Results and conclusions. [/b]Our study demonstrated that chronic inflammation in pulmonary compartment, characterized by the significant increase of proinflammatory cytokines (IL1, IL6, IL10) levels, was the dominant feature of mice response to repeated [i]A. fumigatus[/i] inhalations. The pattern of cytokines’ profile in the course of exposure was similar in both age groups, however in old mice the growth of the cytokines’ levels was more pronounced (especially in case of IL1).
eISSN:1898-2263
ISSN:1232-1966