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RESEARCH PAPER
 
 

[i]Aeromonas hydrophila[/i] subsp. [i]dhakensis[/i] – a causative agent of gastroenteritis imported into the Czech Republic

Eva Krejčí 2,  
 
1
Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Brno, Czech Republic
2
Institute of Public Health, Centre for Clinical Laboratories, Ostrava, Czech Republic
Ann Agric Environ Med 2012;19(3):409–413
KEYWORDS:
ABSTRACT:
Out of the twenty-one [i]A. hydrophila[/i] complex isolates obtained during a routine examination of human diarrhoeal faeces, two [i]A. hydrophila[/i] subsp. [i]dhakensis [/i]isolates (P1097 = CCM 7329 and P1165) were successfully identified by ribotyping. The correct taxonomic position of the [i]A. hydrophila[/i] subsp. [i]dhakensis[/i] CCM 7329 was verified by [i]cpn60[/i] sequencing (GeneBank accession number HM536193). The remaining [i]A. hydrophila[/i] complex isolates were identified as [i]A. hydrophila[/i] subsp. [i]hydrophila[/i]. The ability of biochemical tests and fatty acid methyl ester analysis to reliably discern both [i]A. hydrophila[/i] subsp. [i]dhakensis[/i] and [i]A. hydrophila[/i] subsp. [i]hydrophila[/i] was limited. In contrast to the [i]A. hydrophila[/i] subsp.[i] hydrophila,[/i] the faecal isolates of [i]A. hydrophila[/i] subsp. [i]dhakensis[/i] did not produce acid from arbutin. When compared in a two-dimensional plot, the [i]A. hydrophila[/i] subsp. [i]dhakensis[/i] faecal isolates contained higher amounts of the two minor fatty acids C[sub]13:0[/sub] and C[sub]17:1[/sub] ω8c than the [i]A. hydrophila[/i] subsp. [i]hydrophila[/i] reference strain. This is the first detected occurrence of the less frequent [i]A. hydrophila[/i] subsp. [i]dhakensis [/i]in our region and ribotyping was proved as a suitable method for the identification of [i]A. hydrophila[/i] subsp. [i]dhakensis[/i].
eISSN:1898-2263
ISSN:1232-1966